(2)
(30)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3232, RRID:AB_2834658.
展开/折叠
18 kDa phosphoprotein; CFL 1; CFL; CFL1; COF1_HUMAN; Cofilin 1; Cofilin 1 non muscle; Cofilin; Cofilin non muscle isoform; Cofilin-1; epididymis secretory protein Li 15; HEL-S-15; non-muscle isoform; p18;
抗原和靶标
- P23528 COF1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASGVAVSDGVIKVFNDMKVRKSSTPEEVKKRKKAVLFCLSEDKKNIILEEGKEILVGDVGQTVDDPYATFVKMLPDKDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIYASSKDAIKKKLTGIKHELQANCYEEVKDRCTLAEKLGGSAVISLEGKPL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P23528 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S3 | Phosphorylation | P53671 (LIMK2) , P53667 (LIMK1) , Q13418 (ILK) , Q15139 (PRKD1) , Q96S53 (TESK2) , Q15569 (TESK1) , Q7Z2Y5 (NRK) | Uniprot |
| S8 | Phosphorylation | Uniprot | |
| K13 | Acetylation | Uniprot | |
| K13 | Sumoylation | Uniprot | |
| K13 | Ubiquitination | Uniprot | |
| K19 | Acetylation | Uniprot | |
| K19 | Methylation | Uniprot | |
| K19 | Sumoylation | Uniprot | |
| K19 | Ubiquitination | Uniprot | |
| K22 | Ubiquitination | Uniprot | |
| S23 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| S24 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| T25 | Phosphorylation | Uniprot | |
| K30 | Acetylation | Uniprot | |
| K30 | Sumoylation | Uniprot | |
| K30 | Ubiquitination | Uniprot | |
| K31 | Acetylation | Uniprot | |
| K33 | Acetylation | Uniprot | |
| K34 | Sumoylation | Uniprot | |
| K34 | Ubiquitination | Uniprot | |
| C39 | S-Nitrosylation | Uniprot | |
| S41 | Phosphorylation | Uniprot | |
| K44 | Acetylation | Uniprot | |
| K44 | Sumoylation | Uniprot | |
| K44 | Ubiquitination | Uniprot | |
| K45 | Acetylation | Uniprot | |
| K45 | Sumoylation | Uniprot | |
| K45 | Ubiquitination | Uniprot | |
| K53 | Sumoylation | Uniprot | |
| K53 | Ubiquitination | Uniprot | |
| T63 | Phosphorylation | Uniprot | |
| Y68 | Phosphorylation | P12931 (SRC) | Uniprot |
| T70 | Phosphorylation | Uniprot | |
| K73 | Acetylation | Uniprot | |
| K73 | Ubiquitination | Uniprot | |
| K78 | Acetylation | Uniprot | |
| K78 | Ubiquitination | Uniprot | |
| C80 | S-Nitrosylation | Uniprot | |
| Y82 | Phosphorylation | Uniprot | |
| Y85 | Phosphorylation | Uniprot | |
| T88 | Phosphorylation | Uniprot | |
| Y89 | Phosphorylation | Uniprot | |
| T91 | Phosphorylation | Uniprot | |
| K92 | Acetylation | Uniprot | |
| K92 | Ubiquitination | Uniprot | |
| S94 | Phosphorylation | Uniprot | |
| K95 | Ubiquitination | Uniprot | |
| K96 | Acetylation | Uniprot | |
| K96 | Methylation | Uniprot | |
| K96 | Ubiquitination | Uniprot | |
| S108 | Phosphorylation | Uniprot | |
| K112 | Sumoylation | Uniprot | |
| K112 | Ubiquitination | Uniprot | |
| K114 | Acetylation | Uniprot | |
| K114 | Sumoylation | Uniprot | |
| K114 | Ubiquitination | Uniprot | |
| Y117 | Phosphorylation | Uniprot | |
| S119 | Phosphorylation | Uniprot | |
| S120 | Phosphorylation | Uniprot | |
| K121 | Acetylation | Uniprot | |
| K121 | Sumoylation | Uniprot | |
| K121 | Ubiquitination | Uniprot | |
| K125 | Acetylation | Uniprot | |
| K126 | Acetylation | Uniprot | |
| K127 | Acetylation | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| T129 | Phosphorylation | Uniprot | |
| K132 | Acetylation | Uniprot | |
| K132 | Sumoylation | Uniprot | |
| K132 | Ubiquitination | Uniprot | |
| C139 | S-Nitrosylation | Uniprot | |
| Y140 | Phosphorylation | Uniprot | |
| K144 | Acetylation | Uniprot | |
| K144 | Ubiquitination | Uniprot | |
| C147 | S-Nitrosylation | Uniprot | |
| K152 | Ubiquitination | Uniprot | |
| S156 | Phosphorylation | Uniprot | |
| S160 | Phosphorylation | Uniprot | |
| K164 | Sumoylation | Uniprot | |
| K164 | Ubiquitination | Uniprot |
研究背景
Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. Required for neural tube morphogenesis and neural crest cell migration (By similarity).
Inactivated by phosphorylation on Ser-3. Phosphorylated on Ser-3 in resting cells (By similarity). Dephosphorylated by PDXP/chronophin; this restores its activity in promoting actin filament depolymerization. The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal (By similarity). Phosphorylated via a ARRB1-RAC1-LIMK1-PAK1 cascade upon active ligand stimulation of atypical chemokine receptor ACKR2.
Nucleus matrix. Cytoplasm>Cytoskeleton. Cell projection>Ruffle membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection>Lamellipodium membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection>Lamellipodium.
Note: Colocalizes with the actin cytoskeleton in membrane ruffles and lamellipodia. Detected at the cleavage furrow and contractile ring during cytokinesis. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.
Widely distributed in various tissues.
Can bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is a major component of intranuclear and cytoplasmic actin rods.
Belongs to the actin-binding proteins ADF family.
研究领域
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Pertussis.
· Organismal Systems > Development > Axon guidance. (View pathway)
· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis. (View pathway)
文献引用
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