Phospho-PLD1 (Ser561) Antibody - #AF7371
| 产品: | 磷酸化 PLD1 (Ser561) 抗体 |
| 货号: | AF7371 |
| 描述: | Rabbit polyclonal antibody to Phospho-PLD1 (Ser561) |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| 分子量: | 120kDa; 124kD(Calculated). |
| 蛋白号: | Q13393 |
| RRID: | AB_2843811 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7371, RRID:AB_2843811.
展开/折叠
Choline phosphatase 1; hPLD1; Phosphatidylcholine-hydrolyzing phospholipase D1; Phospholipase D1; PLD 1; PLD1; PLD1_HUMAN;
抗原和靶标
Expressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine.
- Q13393 PLD1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSLKNEPRVNTSALQKIAADMSNIIENLDTRELHFEGEEVDYDVSPSDPKIQEVYIPFSAIYNTQGFKEPNIQTYLSGCPIKAQVLEVERFTSTTRVPSINLYTIELTHGEFKWQVKRKFKHFQEFHRELLKYKAFIRIPIPTRRHTFRRQNVREEPREMPSLPRSSENMIREEQFLGRRKQLEDYLTKILKMPMYRNYHATTEFLDISQLSFIHDLGPKGIEGMIMKRSGGHRIPGLNCCGQGRACYRWSKRWLIVKDSFLLYMKPDSGAIAFVLLVDKEFKIKVGKKETETKYGIRIDNLSRTLILKCNSYRHARWWGGAIEEFIQKHGTNFLKDHRFGSYAAIQENALAKWYVNAKGYFEDVANAMEEANEEIFITDWWLSPEIFLKRPVVEGNRWRLDCILKRKAQQGVRIFIMLYKEVELALGINSEYTKRTLMRLHPNIKVMRHPDHVSSTVYLWAHHEKLVIIDQSVAFVGGIDLAYGRWDDNEHRLTDVGSVKRVTSGPSLGSLPPAAMESMESLRLKDKNEPVQNLPIQKSIDDVDSKLKGIGKPRKFSKFSLYKQLHRHHLHDADSISSIDSTSSYFNHYRSHHNLIHGLKPHFKLFHPSSESEQGLTRPHADTGSIRSLQTGVGELHGETRFWHGKDYCNFVFKDWVQLDKPFADFIDRYSTPRMPWHDIASAVHGKAARDVARHFIQRWNFTKIMKSKYRSLSYPFLLPKSQTTAHELRYQVPGSVHANVQLLRSAADWSAGIKYHEESIHAAYVHVIENSRHYIYIENQFFISCADDKVVFNKIGDAIAQRILKAHRENQKYRVYVVIPLLPGFEGDISTGGGNALQAIMHFNYRTMCRGENSILGQLKAELGNQWINYISFCGLRTHAELEGNLVTELIYVHSKLLIADDNTVIIGSANINDRSMLGKRDSEMAVIVQDTETVPSVMDGKEYQAGRFARGLRLQCFRVVLGYLDDPSEDIQDPVSDKFFKEVWVSTAARNATIYDKVFRCLPNDEVHNLIQLRDFINKPVLAKEDPIRAEEELKKIRGFLVQFPFYFLSEESLLPSVGTKEAIVPMEVWT
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q13393 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| S12 | Phosphorylation | Uniprot | |
| K16 | Ubiquitination | Uniprot | |
| S22 | Phosphorylation | Uniprot | |
| Y42 | Phosphorylation | Uniprot | |
| S45 | Phosphorylation | Uniprot | |
| K50 | Ubiquitination | Uniprot | |
| K121 | Ubiquitination | Uniprot | |
| K132 | Methylation | Uniprot | |
| T147 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| S162 | Phosphorylation | Uniprot | |
| K189 | Ubiquitination | Uniprot | |
| K294 | Ubiquitination | Uniprot | |
| K406 | Methylation | Uniprot | |
| S431 | Phosphorylation | Uniprot | |
| T495 | Phosphorylation | Uniprot | |
| S499 | Phosphorylation | Uniprot | |
| K501 | Ubiquitination | Uniprot | |
| S505 | Phosphorylation | P54646 (PRKAA2) | Uniprot |
| S508 | Phosphorylation | Uniprot | |
| S519 | Phosphorylation | Uniprot | |
| S522 | Phosphorylation | Uniprot | |
| S540 | Phosphorylation | Uniprot | |
| K547 | Ubiquitination | Uniprot | |
| S558 | Phosphorylation | Uniprot | |
| K559 | Ubiquitination | Uniprot | |
| S561 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| Y586 | Phosphorylation | Uniprot | |
| Y590 | Phosphorylation | Uniprot | |
| S610 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S611 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S629 | Phosphorylation | Uniprot | |
| K662 | Ubiquitination | Uniprot | |
| K688 | Ubiquitination | Uniprot | |
| Y711 | Phosphorylation | Uniprot | |
| K796 | Ubiquitination | Uniprot | |
| K807 | Acetylation | Uniprot | |
| K1000 | Ubiquitination | Uniprot | |
| K1027 | Ubiquitination | Uniprot |
研究背景
Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic (By similarity).
Cytoplasm>Perinuclear region. Endoplasmic reticulum membrane>Lipid-anchor>Cytoplasmic side. Golgi apparatus membrane>Lipid-anchor>Cytoplasmic side. Late endosome membrane>Lipid-anchor>Cytoplasmic side.
Expressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine.
Interacts with PIP5K1B.
Belongs to the phospholipase D family.
研究领域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway. (View pathway)
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Pancreatic cancer. (View pathway)
· Human Diseases > Cancers: Overview > Choline metabolism in cancer. (View pathway)
· Metabolism > Lipid metabolism > Glycerophospholipid metabolism.
· Metabolism > Lipid metabolism > Ether lipid metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis. (View pathway)
· Organismal Systems > Nervous system > Glutamatergic synapse.
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