产品: 磷酸化 LATS2 (Ser380) 抗体
货号: AF7439
描述: Rabbit polyclonal antibody to Phospho-LATS2 (Ser380)
应用: WB IF/ICC
反应: Human, Mouse
预测: Pig, Bovine, Horse, Rabbit, Dog
分子量: 150kDa; 120kD(Calculated).
蛋白号: Q9NRM7
RRID: AB_2843879

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   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(92%), Bovine(91%), Horse(92%), Rabbit(100%), Dog(92%)
克隆:
Polyclonal
特异性:
Phospho-LATS2 (Ser380) Antibody detects endogenous levels of LATS2 only when phosphorylated at Ser380.
RRID:
AB_2843879
引用格式: Affinity Biosciences Cat# AF7439, RRID:AB_2843879.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

FLJ13161; Kinase phosphorylated during mitosis protein; KPM; Large tumor suppressor homolog 2; Large tumor suppressor homolog 2 Drosophila; Large tumor suppressor kinase 2; LATS large tumor suppressor Drosophila homolog 2; LATS large tumor suppressor homolog 2; Lats2; LATS2_HUMAN; Serine/threonine kinase kpm; Serine/threonine protein kinase kpm; Serine/threonine-protein kinase kpm; Serine/threonine-protein kinase LATS2; Warts like kinase; Warts-like kinase;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q9NRM7 LATS2_HUMAN:

Expressed at high levels in heart and skeletal muscle and at lower levels in all other tissues examined.

序列:
MRPKTFPATTYSGNSRQRLQEIREGLKQPSKSSVQGLPAGPNSDTSLDAKVLGSKDATRQQQQMRATPKFGPYQKALREIRYSLLPFANESGTSAAAEVNRQMLQELVNAGCDQEMAGRALKQTGSRSIEAALEYISKMGYLDPRNEQIVRVIKQTSPGKGLMPTPVTRRPSFEGTGDSFASYHQLSGTPYEGPSFGADGPTALEEMPRPYVDYLFPGVGPHGPGHQHQHPPKGYGASVEAAGAHFPLQGAHYGRPHLLVPGEPLGYGVQRSPSFQSKTPPETGGYASLPTKGQGGPPGAGLAFPPPAAGLYVPHPHHKQAGPAAHQLHVLGSRSQVFASDSPPQSLLTPSRNSLNVDLYELGSTSVQQWPAATLARRDSLQKPGLEAPPRAHVAFRPDCPVPSRTNSFNSHQPRPGPPGKAEPSLPAPNTVTAVTAAHILHPVKSVRVLRPEPQTAVGPSHPAWVPAPAPAPAPAPAPAAEGLDAKEEHALALGGAGAFPLDVEYGGPDRRCPPPPYPKHLLLRSKSEQYDLDSLCAGMEQSLRAGPNEPEGGDKSRKSAKGDKGGKDKKQIQTSPVPVRKNSRDEEKRESRIKSYSPYAFKFFMEQHVENVIKTYQQKVNRRLQLEQEMAKAGLCEAEQEQMRKILYQKESNYNRLKRAKMDKSMFVKIKTLGIGAFGEVCLACKVDTHALYAMKTLRKKDVLNRNQVAHVKAERDILAEADNEWVVKLYYSFQDKDSLYFVMDYIPGGDMMSLLIRMEVFPEHLARFYIAELTLAIESVHKMGFIHRDIKPDNILIDLDGHIKLTDFGLCTGFRWTHNSKYYQKGSHVRQDSMEPSDLWDDVSNCRCGDRLKTLEQRARKQHQRCLAHSLVGTPNYIAPEVLLRKGYTQLCDWWSVGVILFEMLVGQPPFLAPTPTETQLKVINWENTLHIPAQVKLSPEARDLITKLCCSADHRLGRNGADDLKAHPFFSAIDFSSDIRKQPAPYVPTISHPMDTSNFDPVDEESPWNDASEGSTKAWDTLTSPNNKHPEHAFYEFTFRRFFDDNGYPFRCPKPSGAEASQAESSDLESSDLVDQTEGCQPVYV

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Rabbit
100
Pig
92
Horse
92
Dog
92
Bovine
91
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9NRM7 作为底物

Site PTM Type Enzyme
T5 Phosphorylation
T9 Phosphorylation
T10 Phosphorylation
Y11 Phosphorylation
S12 Phosphorylation
S43 Phosphorylation
K50 Ubiquitination
K55 Acetylation
K55 Ubiquitination
Y82 Phosphorylation
S83 Phosphorylation O14965 (AURKA)
Y135 Phosphorylation
K154 Ubiquitination
S172 Phosphorylation O14757 (CHEK1) , O96017 (CHEK2)
S274 Phosphorylation
K278 Ubiquitination
T279 Phosphorylation
Y286 Phosphorylation
S288 Phosphorylation
S342 Phosphorylation
S380 Phosphorylation O14965 (AURKA) , O14757 (CHEK1) , O96017 (CHEK2)
S408 Phosphorylation O96017 (CHEK2) , O14757 (CHEK1)
S446 Phosphorylation O96017 (CHEK2) , O14757 (CHEK1)
S528 Phosphorylation
Y531 Phosphorylation
S535 Phosphorylation
S543 Phosphorylation
K565 Acetylation
K570 Acetylation
K571 Acetylation
T575 Phosphorylation
S576 Phosphorylation
S598 Phosphorylation
K620 Ubiquitination
K651 Ubiquitination
T690 Phosphorylation
K697 Ubiquitination
K793 Ubiquitination
K827 Ubiquitination
S829 Phosphorylation
S835 Phosphorylation Q9NRM7 (LATS2) , O14757 (CHEK1)
S872 Phosphorylation Q13043 (STK4) , Q13188 (STK3)
K939 Ubiquitination
K950 Ubiquitination
K968 Ubiquitination
K984 Ubiquitination
T1024 Phosphorylation
T1026 Phosphorylation
S1027 Phosphorylation
Y1038 Phosphorylation
T1041 Phosphorylation Q13188 (STK3) , Q13043 (STK4)
Y1051 Phosphorylation

翻译修饰 - Q9NRM7 作为激酶

Substrate Site Source
O95863 (SNAI1) T203 Uniprot
P00519-2 (ABL1) T197 Uniprot
P38936 (CDKN1A) S146 Uniprot
P46937 (YAP1) S127 Uniprot
P46937 (YAP1) S397 Uniprot
P61981 (YWHAG) S59 Uniprot
Q02241 (KIF23) S716 Uniprot
Q02241 (KIF23) S814 Uniprot
Q4VCS5 (AMOT) S175 Uniprot
Q8NHZ8 (CDC26) T7 Uniprot
Q9GZV5 (WWTR1) S89 Uniprot
Q9NQS7 (INCENP) S894 Uniprot
Q9NRM7 (LATS2) S835 Uniprot
Q9Y2J4 (AMOTL2) S159 Uniprot

研究背景

功能:

Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Negative regulator of the androgen receptor. Phosphorylates SNAI1 in the nucleus leading to its nuclear retention and stabilization, which enhances its epithelial-mesenchymal transition and tumor cell invasion/migration activities. This tumor-promoting activity is independent of its effects upon YAP1 or WWTR1/TAZ.

翻译修饰:

Autophosphorylated and phosphorylated during M-phase and the G1/S-phase of the cell cycle. Phosphorylated and activated by STK3/MST2.

细胞定位:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm. Cytoplasm>Cytoskeleton>Spindle pole. Nucleus.
Note: Colocalizes with AURKA at the centrosomes during interphase, early prophase and cytokinesis. Migrates to the spindle poles during mitosis, and to the midbody during cytokinesis. Translocates to the nucleus upon mitotic stress by nocodazole treatment.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed at high levels in heart and skeletal muscle and at lower levels in all other tissues examined.

亚基结构:

Interacts with and is phosphorylated by AURKA. Binds to AR. Interacts with AJUBA during mitosis and this complex regulates organization of the spindle apparatus through recruitment of gamma-tubulin to the centrosome. Interacts (via PPxY motif) with YAP1 (via WW domains). Interacts with MOB1A and MOB1B. Interacts with LIMD1, WTIP and AJUBA. Interacts with SNAI1.

蛋白家族:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

研究领域

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway - multiple species.   (View pathway)

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