Phospho-Claudin 7 (Tyr210) Antibody - #AF7453
| 产品: | 磷酸化 Claudin 7 (Tyr210) 抗体 |
| 货号: | AF7453 |
| 描述: | Rabbit polyclonal antibody to Phospho-Claudin 7 (Tyr210) |
| 应用: | WB IHC |
| 反应: | Human, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 22kDa; 22kD(Calculated). |
| 蛋白号: | O95471 |
| RRID: | AB_2843893 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7453, RRID:AB_2843893.
展开/折叠
CEPTR L2; CEPTRL 2; CEPTRL2; Claudin 1; Claudin 9; Claudin-7; Claudin1; Claudin7; Claudin9; CLD7_HUMAN; CLDN 7; CLDN-7; CLDN7; Clostridium perfringens enterotoxin receptor like 2; CPETR L2; CPETRL 2; CPETRL2; Hs.84359;
抗原和靶标
Expressed in kidney, lung and prostate. Isoform 1 seems to be predominant, except in some normal prostate samples, where isoform 2 is the major form. Down-regulated in breast cancers, including ductal carcinoma in situ (DCIS), lobular carcinoma in situ (LCIS) and invasive ductal carcinoma (IDC) (at protein level), as well as in several cancer cell lines. Loss of expression correlates with histological grade, occurring predominantly in high-grade lesions.
- O95471 CLD7_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MANSGLQLLGFSMALLGWVGLVACTAIPQWQMSSYAGDNIITAQAMYKGLWMDCVTQSTGMMSCKMYDSVLALSAALQATRALMVVSLVLGFLAMFVATMGMKCTRCGGDDKVKKARIAMGGGIIFIVAGLAALVACSWYGHQIVTDFYNPLIPTNIKYEFGPAIFIGWAGSALVILGGALLSCSCPGNESKAGYRVPRSYPKSNSSKEYV
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O95471 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y67 | Phosphorylation | Uniprot | |
| T99 | Phosphorylation | Uniprot | |
| Y195 | Phosphorylation | Uniprot | |
| S200 | Phosphorylation | Uniprot | |
| K203 | Ubiquitination | Uniprot | |
| S204 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| S206 | Phosphorylation | Q96J92 (WNK4) | Uniprot |
| S207 | Phosphorylation | Uniprot | |
| K208 | Ubiquitination | Uniprot | |
| Y210 | Phosphorylation | Uniprot |
研究背景
Plays a major role in tight junction-specific obliteration of the intercellular space.
Phosphorylated.
Cell membrane>Multi-pass membrane protein. Basolateral cell membrane. Cell junction>Tight junction.
Note: Co-localizes with EPCAM at the basolateral cell membrane and tight junction.
Expressed in kidney, lung and prostate. Isoform 1 seems to be predominant, except in some normal prostate samples, where isoform 2 is the major form. Down-regulated in breast cancers, including ductal carcinoma in situ (DCIS), lobular carcinoma in situ (LCIS) and invasive ductal carcinoma (IDC) (at protein level), as well as in several cancer cell lines. Loss of expression correlates with histological grade, occurring predominantly in high-grade lesions.
Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3 (By similarity). The phosphorylated form interacts with EPCAM. Does not interact with CD81.
Belongs to the claudin family.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs). (View pathway)
· Human Diseases > Infectious diseases: Viral > Hepatitis C.
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
限制条款
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