产品: MOB1A 抗体
货号: AF7914
描述: Rabbit polyclonal antibody to MOB1A
应用: WB
反应: Human, Mouse
预测: Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 25kDa; 25kD(Calculated).
蛋白号: Q7L9L4
RRID: AB_2844277

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(92%)
克隆:
Polyclonal
特异性:
MOB1A Antibody detects endogenous levels of total MOB1A.
RRID:
AB_2844277
引用格式: Affinity Biosciences Cat# AF7914, RRID:AB_2844277.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

MATS 2; MATS2; MGC33910; Mob 1A; Mob 1B; MOB 4A; MOB kinase activator 1B; Mob1 homolog 1A; MOB1 Mps One Binder homolog B; MOB1 Mps one binder kinase activator like 1A; Mob1A; Mob1B; MOBKL 1A; MOBKL1A; MOL1A_HUMAN; Mps one binder kinase activator like 1A; Mps one binder kinase activator-like 1A; Protein Mob4A;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q7L9L4 MOB1B_HUMAN:

Adrenal gland, bone marrow, brain, lung, placenta, prostate, salivary gland, skeletal muscle, testis, thymus, thyroid gland, uterus, colon with mucosa, fetal brain and fetal liver.

序列:
MSFLFGSRSSKTFKPKKNIPEGSHQYELLKHAEATLGSGNLRMAVMLPEGEDLNEWVAVNTVDFFNQINMLYGTITDFCTEESCPVMSAGPKYEYHWADGTNIKKPIKCSAPKYIDYLMTWVQDQLDDETLFPSKIGVPFPKNFMSVAKTILKRLFRVYAHIYHQHFDPVIQLQEEAHLNTSFKHFIFFVQEFNLIDRRELAPLQELIEKLTSKDR

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
92
Pig
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q7L9L4 作为底物

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
S7 Phosphorylation
S9 Phosphorylation
S10 Phosphorylation
K11 Acetylation
T12 Phosphorylation Q13043 (STK4) , Q13188 (STK3)
K16 Acetylation
K17 Acetylation
K17 Ubiquitination
S23 Phosphorylation
Y26 Phosphorylation
K30 Ubiquitination
T35 Phosphorylation Q13188 (STK3) , Q13043 (STK4)
S38 Phosphorylation
T74 Phosphorylation Q13188 (STK3)
Y95 Phosphorylation
K142 Ubiquitination
K149 Acetylation

研究背景

功能:

Activator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Stimulates the kinase activity of STK38L.

翻译修饰:

Phosphorylated by STK3/MST2 and STK4/MST1 and this phosphorylation enhances its binding to LATS1.

细胞定位:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Adrenal gland, bone marrow, brain, lung, placenta, prostate, salivary gland, skeletal muscle, testis, thymus, thyroid gland, uterus, colon with mucosa, fetal brain and fetal liver.

亚基结构:

Binds STK38L. Interacts with LATS1 and LATS2.

蛋白家族:

Belongs to the MOB1/phocein family.

研究领域

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway - multiple species.   (View pathway)

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