(2)
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| 产品: | LATS2 抗体 |
| 货号: | AF7940 |
| 描述: | Rabbit polyclonal antibody to LATS2 |
| 应用: | WB |
| 反应: | Human, Mouse |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| 分子量: | 150kDa; 120kD(Calculated). |
| 蛋白号: | Q9NRM7 |
| RRID: | AB_2844303 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7940, RRID:AB_2844303.
展开/折叠
FLJ13161; Kinase phosphorylated during mitosis protein; KPM; Large tumor suppressor homolog 2; Large tumor suppressor homolog 2 Drosophila; Large tumor suppressor kinase 2; LATS large tumor suppressor Drosophila homolog 2; LATS large tumor suppressor homolog 2; Lats2; LATS2_HUMAN; Serine/threonine kinase kpm; Serine/threonine protein kinase kpm; Serine/threonine-protein kinase kpm; Serine/threonine-protein kinase LATS2; Warts like kinase; Warts-like kinase;
抗原和靶标
Expressed at high levels in heart and skeletal muscle and at lower levels in all other tissues examined.
- Q9NRM7 LATS2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRPKTFPATTYSGNSRQRLQEIREGLKQPSKSSVQGLPAGPNSDTSLDAKVLGSKDATRQQQQMRATPKFGPYQKALREIRYSLLPFANESGTSAAAEVNRQMLQELVNAGCDQEMAGRALKQTGSRSIEAALEYISKMGYLDPRNEQIVRVIKQTSPGKGLMPTPVTRRPSFEGTGDSFASYHQLSGTPYEGPSFGADGPTALEEMPRPYVDYLFPGVGPHGPGHQHQHPPKGYGASVEAAGAHFPLQGAHYGRPHLLVPGEPLGYGVQRSPSFQSKTPPETGGYASLPTKGQGGPPGAGLAFPPPAAGLYVPHPHHKQAGPAAHQLHVLGSRSQVFASDSPPQSLLTPSRNSLNVDLYELGSTSVQQWPAATLARRDSLQKPGLEAPPRAHVAFRPDCPVPSRTNSFNSHQPRPGPPGKAEPSLPAPNTVTAVTAAHILHPVKSVRVLRPEPQTAVGPSHPAWVPAPAPAPAPAPAPAAEGLDAKEEHALALGGAGAFPLDVEYGGPDRRCPPPPYPKHLLLRSKSEQYDLDSLCAGMEQSLRAGPNEPEGGDKSRKSAKGDKGGKDKKQIQTSPVPVRKNSRDEEKRESRIKSYSPYAFKFFMEQHVENVIKTYQQKVNRRLQLEQEMAKAGLCEAEQEQMRKILYQKESNYNRLKRAKMDKSMFVKIKTLGIGAFGEVCLACKVDTHALYAMKTLRKKDVLNRNQVAHVKAERDILAEADNEWVVKLYYSFQDKDSLYFVMDYIPGGDMMSLLIRMEVFPEHLARFYIAELTLAIESVHKMGFIHRDIKPDNILIDLDGHIKLTDFGLCTGFRWTHNSKYYQKGSHVRQDSMEPSDLWDDVSNCRCGDRLKTLEQRARKQHQRCLAHSLVGTPNYIAPEVLLRKGYTQLCDWWSVGVILFEMLVGQPPFLAPTPTETQLKVINWENTLHIPAQVKLSPEARDLITKLCCSADHRLGRNGADDLKAHPFFSAIDFSSDIRKQPAPYVPTISHPMDTSNFDPVDEESPWNDASEGSTKAWDTLTSPNNKHPEHAFYEFTFRRFFDDNGYPFRCPKPSGAEASQAESSDLESSDLVDQTEGCQPVYV
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9NRM7 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T5 | Phosphorylation | Uniprot | |
| T9 | Phosphorylation | Uniprot | |
| T10 | Phosphorylation | Uniprot | |
| Y11 | Phosphorylation | Uniprot | |
| S12 | Phosphorylation | Uniprot | |
| S43 | Phosphorylation | Uniprot | |
| K50 | Ubiquitination | Uniprot | |
| K55 | Acetylation | Uniprot | |
| K55 | Ubiquitination | Uniprot | |
| Y82 | Phosphorylation | Uniprot | |
| S83 | Phosphorylation | O14965 (AURKA) | Uniprot |
| Y135 | Phosphorylation | Uniprot | |
| K154 | Ubiquitination | Uniprot | |
| S172 | Phosphorylation | O14757 (CHEK1) , O96017 (CHEK2) | Uniprot |
| S274 | Phosphorylation | Uniprot | |
| K278 | Ubiquitination | Uniprot | |
| T279 | Phosphorylation | Uniprot | |
| Y286 | Phosphorylation | Uniprot | |
| S288 | Phosphorylation | Uniprot | |
| S342 | Phosphorylation | Uniprot | |
| S380 | Phosphorylation | O14965 (AURKA) , O14757 (CHEK1) , O96017 (CHEK2) | Uniprot |
| S408 | Phosphorylation | O96017 (CHEK2) , O14757 (CHEK1) | Uniprot |
| S446 | Phosphorylation | O96017 (CHEK2) , O14757 (CHEK1) | Uniprot |
| S528 | Phosphorylation | Uniprot | |
| Y531 | Phosphorylation | Uniprot | |
| S535 | Phosphorylation | Uniprot | |
| S543 | Phosphorylation | Uniprot | |
| K565 | Acetylation | Uniprot | |
| K570 | Acetylation | Uniprot | |
| K571 | Acetylation | Uniprot | |
| T575 | Phosphorylation | Uniprot | |
| S576 | Phosphorylation | Uniprot | |
| S598 | Phosphorylation | Uniprot | |
| K620 | Ubiquitination | Uniprot | |
| K651 | Ubiquitination | Uniprot | |
| T690 | Phosphorylation | Uniprot | |
| K697 | Ubiquitination | Uniprot | |
| K793 | Ubiquitination | Uniprot | |
| K827 | Ubiquitination | Uniprot | |
| S829 | Phosphorylation | Uniprot | |
| S835 | Phosphorylation | Q9NRM7 (LATS2) , O14757 (CHEK1) | Uniprot |
| S872 | Phosphorylation | Q13043 (STK4) , Q13188 (STK3) | Uniprot |
| K939 | Ubiquitination | Uniprot | |
| K950 | Ubiquitination | Uniprot | |
| K968 | Ubiquitination | Uniprot | |
| K984 | Ubiquitination | Uniprot | |
| T1024 | Phosphorylation | Uniprot | |
| T1026 | Phosphorylation | Uniprot | |
| S1027 | Phosphorylation | Uniprot | |
| Y1038 | Phosphorylation | Uniprot | |
| T1041 | Phosphorylation | Q13188 (STK3) , Q13043 (STK4) | Uniprot |
| Y1051 | Phosphorylation | Uniprot |
翻译修饰 - Q9NRM7 作为激酶
| Substrate | Site | Source |
|---|---|---|
| O95863 (SNAI1) | T203 | Uniprot |
| P00519-2 (ABL1) | T197 | Uniprot |
| P38936 (CDKN1A) | S146 | Uniprot |
| P46937 (YAP1) | S127 | Uniprot |
| P46937 (YAP1) | S397 | Uniprot |
| P61981 (YWHAG) | S59 | Uniprot |
| Q02241 (KIF23) | S716 | Uniprot |
| Q02241 (KIF23) | S814 | Uniprot |
| Q4VCS5 (AMOT) | S175 | Uniprot |
| Q8NHZ8 (CDC26) | T7 | Uniprot |
| Q9GZV5 (WWTR1) | S89 | Uniprot |
| Q9NQS7 (INCENP) | S894 | Uniprot |
| Q9NRM7 (LATS2) | S835 | Uniprot |
| Q9Y2J4 (AMOTL2) | S159 | Uniprot |
研究背景
Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Negative regulator of the androgen receptor. Phosphorylates SNAI1 in the nucleus leading to its nuclear retention and stabilization, which enhances its epithelial-mesenchymal transition and tumor cell invasion/migration activities. This tumor-promoting activity is independent of its effects upon YAP1 or WWTR1/TAZ.
Autophosphorylated and phosphorylated during M-phase and the G1/S-phase of the cell cycle. Phosphorylated and activated by STK3/MST2.
Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm. Cytoplasm>Cytoskeleton>Spindle pole. Nucleus.
Note: Colocalizes with AURKA at the centrosomes during interphase, early prophase and cytokinesis. Migrates to the spindle poles during mitosis, and to the midbody during cytokinesis. Translocates to the nucleus upon mitotic stress by nocodazole treatment.
Expressed at high levels in heart and skeletal muscle and at lower levels in all other tissues examined.
Interacts with and is phosphorylated by AURKA. Binds to AR. Interacts with AJUBA during mitosis and this complex regulates organization of the spindle apparatus through recruitment of gamma-tubulin to the centrosome. Interacts (via PPxY motif) with YAP1 (via WW domains). Interacts with MOB1A and MOB1B. Interacts with LIMD1, WTIP and AJUBA. Interacts with SNAI1.
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
研究领域
· Environmental Information Processing > Signal transduction > Hippo signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Hippo signaling pathway - multiple species. (View pathway)
文献引用
Application: WB Species: human Sample: NCI-H460、A549 cells and p53-deficient NCI-H1299 cells
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