产品: PHGDH 抗体
货号: AF4618
描述: Rabbit polyclonal antibody to PHGDH
应用: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 57kDa; 57kD(Calculated).
蛋白号: O43175
RRID: AB_2844565

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(92%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
PHGDH Antibody detects endogenous levels of total PHGDH.
RRID:
AB_2844565
引用格式: Affinity Biosciences Cat# AF4618, RRID:AB_2844565.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

3 PGDH; 3-PGDH; 3-phosphoglycerate dehydrogenase; 3PGDH; D-3-phosphoglycerate dehydrogenase; EC 1.1.1.95; Epididymis secretory protein Li 113; HEL S 113; NLS; NLS1; PDG; PGAD; PGD; PGDH; PGDH3; Phgdh; PHGDHD; Phosphoglycerate dehydrogenase; SERA; SERA_HUMAN;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
序列:
MAFANLRKVLISDSLDPCCRKILQDGGLQVVEKQNLSKEELIAELQDCEGLIVRSATKVTADVINAAEKLQVVGRAGTGVDNVDLEAATRKGILVMNTPNGNSLSAAELTCGMIMCLARQIPQATASMKDGKWERKKFMGTELNGKTLGILGLGRIGREVATRMQSFGMKTIGYDPIISPEVSASFGVQQLPLEEIWPLCDFITVHTPLLPSTTGLLNDNTFAQCKKGVRVVNCARGGIVDEGALLRALQSGQCAGAALDVFTEEPPRDRALVDHENVISCPHLGASTKEAQSRCGEEIAVQFVDMVKGKSLTGVVNAQALTSAFSPHTKPWIGLAEALGTLMRAWAGSPKGTIQVITQGTSLKNAGNCLSPAVIVGLLKEASKQADVNLVNAKLLVKEAGLNVTTSHSPAAPGEQGFGECLLAVALAGAPYQAVGLVQGTTPVLQGLNGAVFRPEVPLRRDLPLLLFRTQTSDPAMLPTMIGLLAEAGVRLLSYQTSLVSDGETWHVMGISSLLPSLEAWKQHVTEAFQFHF

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Zebrafish
92
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O43175 作为底物

Site PTM Type Enzyme
R7 Methylation
K8 Ubiquitination
S14 Phosphorylation
K21 Sumoylation
K21 Ubiquitination
K33 Acetylation
K33 Ubiquitination
K38 Ubiquitination
S55 Phosphorylation Q05513 (PRKCZ)
T57 Phosphorylation Q05513 (PRKCZ)
K58 Ubiquitination
T60 Phosphorylation
K69 Ubiquitination
R75 Methylation
T78 Phosphorylation Q05513 (PRKCZ)
T89 Phosphorylation
S105 Phosphorylation
T125 Phosphorylation
S127 Phosphorylation
K129 Ubiquitination
K137 Ubiquitination
T141 Phosphorylation
K146 Ubiquitination
T147 Phosphorylation
R236 Methylation
R247 Methylation
C281 S-Nitrosylation
K289 Acetylation
K289 Ubiquitination
K308 Ubiquitination
S326 Phosphorylation
S349 Phosphorylation
K351 Ubiquitination
T353 Phosphorylation
T358 Phosphorylation
S362 Phosphorylation
K364 Ubiquitination
C369 S-Nitrosylation
S371 Phosphorylation
K380 Ubiquitination
K384 Ubiquitination
K394 Ubiquitination
K398 Ubiquitination
S473 Phosphorylation
T480 Phosphorylation
K522 Ubiquitination

研究背景

功能:

Catalyzes the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, the first step of the phosphorylated L-serine biosynthesis pathway. Also catalyzes the reversible oxidation of 2-hydroxyglutarate to 2-oxoglutarate and the reversible oxidation of (S)-malate to oxaloacetate.

亚基结构:

Homotetramer.

蛋白家族:

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

研究领域

· Metabolism > Amino acid metabolism > Glycine, serine and threonine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

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