CPT1A Antibody - #DF12004

Fig. 4 Dihydroartemisinin reduces c-Myc transcriptional activity, and its downstream targets involved in lipid metabolism. A c-Myc reporter activity in DLD-1 and HCT116 cells after dihydroartemisinin (DHA, 10 µM) treatment. The data are shown as means ± SEM, n = 3 independent experiments, *p 

Fig. 6. β-PAE promotes the expression of hepatic lipid oxidation-related proteins and genes in HFD-fed rats. (A–G) Western blot analysis on the expression of SIRT1, PGC-1α, PPARα, FGF21, CPT-1a and ACOX1; (H–K) The mRNA expression of SIRT1, PPARα, CPT-1a and ACOX1. Data are presented as the mean ± SD (n = 6~8). ##p < 0.01 vs. NC group; *p < 0.05, **p < 0.01 vs. Model group.

Fig. 5. Effects ofL. plantarum Q16 on key proteins involved in hepatic lipid metabolism in HFD-fed obese mice. Data are presented as mean ± SD (n = 6). Different lowercase alphabet letters were significantly different at the level of P < 0.05.

FIGURE 2 Roxadustat attenuates lipid accumulation by increasing PPARα protein expression in the liver and reducing FASN levels in HepG2 cells. (A–D) Liver samples were collected from mice in Figure 1 and used for the following experiments. Liver frozen sections were stained with Oil Red O staining (A); triglyceride content was determined using an assay kit (B); mRNA expression of DGAT1 and FASN was determined by qRT-PCR (C); protein expression of PPARα and CPT1A was determined by Western blot with quantitative analysis of band density (D); (E) HepG2 cells were treated with roxadustat at indicated concentrations for 24 h. Protein expression of FASN and HIF-1α was determined by Western blot with quantitative analysis of band density (right panels); (F,G) HepG2 cells were transfected with scrambled siRNA (si-NC) or HIF-1α siRNA (si-HIF-1α) for 24 h, and then treated with roxadustat for 24 h. Protein expression of FASN, PPARα, and HIF-1α was determined by Western blot with quantitative analysis of band density (F); lipid accumulation was determined by Oil Red O staining with quantitative analysis (G). *, p < 0.05; **, p < 0.01; ***, p < 0.001 vs ctrl; # p < 0.05, ###, p < 0.001 vs ALD group (n ≥ 5); Roxa: roxadustat.

Figure 5: |CADE regulates lipid synthesis, decomposition and transport through miR-378b in C57BL/6 mice. (a,b) Western blot analysis of the protein expressions of SREBP-1c, FASN, PPARα, CPT1, MTTP and protein ratio of p-foxO1/foxO1 when the mice were injected with AAV-miR-378b agomir and antagomir. All data are expressed as the means ± SD of at least three separate experiments.