PFKFB3 Antibody | Affinity Biosciences LTD|亲科生物官网

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Cites

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Western blot analysis of extracts from A549 cells, using PFKFB3-Specific antibody.

Western blot analysis of extracts from Hybridoma cells, using PFKFB3-Specific Antibody.

DF12016 at 1/100 staining Rat heart tissue by IHC-P.

Fig.

产品:	PFKFB3 抗体
货号:	DF12016
描述:	Rabbit polyclonal antibody to PFKFB3
应用:	WB IHC
反应:	Human, Mouse, Rat
分子量:	58 kDa; 60kD(Calculated).
蛋白号:	Q16875
RRID:	AB_2844821

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
50ul	RMB¥ 1250	现货
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat

克隆:

Polyclonal

特异性:

PFKFB3 Antibody detects endogenous levels of total PFKFB3.

RRID:

AB_2844821
引用格式: Affinity Biosciences Cat# DF12016, RRID:AB_2844821.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

6 phosphofructo 2 kinase/ fructose 2,6 bisphosphatase; 6 phosphofructo 2 kinase/fructose 2,6 biphosphatase 3; 6-bisphosphatase; 6-P2ase 3; 6-P2ASE brain/placenta-type isozyme; 6PF 2 K/Fru 2,6 P2ASE brain/placenta type isozyme; 6PF 2-K/Fru 2,6 P2ase 3; 6PF-2-K/Fru-2; F263_HUMAN; fructose 6 phosphate,2 kinase/fructose 2, 6 bisphosphatase; Fructose-2; Inducible 6 phosphofructo 2 kinase/fructose 2,6 bisphosphatase; iPFK 2; iPFK-2; IPFK2; PFK/FBPase 3; PFK2; PFKFB3; Renal carcinoma antigen NY REN 56; Renal carcinoma antigen NY-REN-56; uPFK 2;

抗原和靶标

免疫原:

Uniprot:

Q16875(F263_HUMAN) >>Visit HPA database.

基因/基因ID:

PFKFB3(5209)

表达:

Q16875 F263_HUMAN:

Ubiquitous.

序列:

Q16875 F263_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPLELTQSRVQKIWVPVDHRPSLPRSCGPKLTNSPTVIVMVGLPARGKTYISKKLTRYLNWIGVPTKVFNVGEYRREAVKQYSSYNFFRPDNEEAMKVRKQCALAALRDVKSYLAKEGGQIAVFDATNTTRERRHMILHFAKENDFKAFFIESVCDDPTVVASNIMEVKISSPDYKDCNSAEAMDDFMKRISCYEASYQPLDPDKCDRDLSLIKVIDVGRRFLVNRVQDHIQSRIVYYLMNIHVQPRTIYLCRHGENEHNLQGRIGGDSGLSSRGKKFASALSKFVEEQNLKDLRVWTSQLKSTIQTAEALRLPYEQWKALNEIDAGVCEELTYEEIRDTYPEEYALREQDKYYYRYPTGESYQDLVQRLEPVIMELERQENVLVICHQAVLRCLLAYFLDKSAEEMPYLKCPLHTVLKLTPVAYGCRVESIYLNVESVCTHRERSEDAKKGPNPLMRRNSVTPLASPEPTKKPRINSFEEHVASTSAALPSCLPPEVPTQLPGQNMKGSRSSADSSRKH

翻译修饰 - Q16875 作为底物

Q16875

Site	PTM Type	Enzyme	Source
K12	Ubiquitination		Uniprot
S22	Phosphorylation		Uniprot
K97	Ubiquitination		Uniprot
K100	Ubiquitination		Uniprot
K111	Ubiquitination		Uniprot
K116	Ubiquitination		Uniprot
S172	Phosphorylation		Uniprot
K176	Ubiquitination		Uniprot
K189	Ubiquitination		Uniprot
Y194	Phosphorylation		Uniprot
K205	Ubiquitination		Uniprot
K214	Ubiquitination		Uniprot
S269	Phosphorylation	O14920 (IKBKB)	Uniprot
S273	Phosphorylation		Uniprot
K277	Ubiquitination		Uniprot
S283	Phosphorylation		Uniprot
K284	Ubiquitination		Uniprot
K292	Ubiquitination		Uniprot
K302	Ubiquitination		Uniprot
K319	Ubiquitination		Uniprot
K352	Ubiquitination		Uniprot
K411	Ubiquitination		Uniprot
S461	Phosphorylation	Q15418 (RPS6KA1) , P17612 (PRKACA) , Q13131 (PRKAA1) , P17252 (PRKCA) , P49137 (MAPKAPK2) , P31749 (AKT1)	Uniprot
T463	Phosphorylation		Uniprot
S467	Phosphorylation		Uniprot
T471	Phosphorylation		Uniprot
S478	Phosphorylation		Uniprot

研究背景

Q16875_F263_HUMAN

功能:

Synthesis and degradation of fructose 2,6-bisphosphate.

翻译修饰:

Phosphorylation by AMPK stimulates activity.

组织特异性:

Ubiquitous.

蛋白家族:

In the C-terminal section; belongs to the phosphoglycerate mutase family.

研究领域

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)

· Metabolism > Carbohydrate metabolism > Fructose and mannose metabolism.

文献引用

1). Yes associated protein 1 promotes resistance to 5-fluorouracil in gastric cancer by regulating GLUT3-dependent glycometabolism reprogramming of tumor-associated macrophages. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS (PubMed: 33727040) [IF=3.9]

Application: WB Species: Human Sample: Gastric cancer (GC) cells

Fig. 3. IL13 secreted by YAP1-overexpressed GC cells stimulates resistance to 5-FU via inducing M2 subtype macrophage glycolysis reprogramming. A-C. RT-PCR was used to detect the mRNA expression of glycolysis enzymes, fatty acid and amino acid metabolism enzymes in THP1 after co-cultured with MKN-YAP1 or MKN45-Vetor. D. Protein level change of glycolysis enzymes in THP1 after co-cultured with MKN-YAP1, MKN45-Vetor, SGC7901-siYAP1 or SGC7901-NC. E. RT-PCR revealed the mRNA expression of glycolysis enzymes and M2 TAMs markers in THP1 after co-cultured with SGC7901-siYAP1 or SGC7901-NC. F-G. Relative lactate release from cells was determined by colorimetric analysis. Relative glucose uptake cells by Flow cytometry detection. All data presented are the mean ± SD (*p < 0.05, **p < 0.01) of triplicate determination from three independent experiments.

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PFKFB3 Antibody - #DF12016

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