产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12100, RRID:AB_2844905.
展开/折叠
3 hydroxy 3 methylglutaryl Coenzyme A synthase 1 (soluble); 3-hydroxy-3-methylglutaryl coenzyme A synthase; cytoplasmic; EC 2.3.3.10; HMCS1_HUMAN; HMG CoA synthase; HMG-CoA synthase; HMGCS; HMGCS1; Hydroxymethylglutaryl CoA synthase, cytoplasmic; Hydroxymethylglutaryl-CoA synthase; MGC90332;
抗原和靶标
- Q01581 HMCS1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPGSLPLNAEACWPKDVGIVALEIYFPSQYVDQAELEKYDGVDAGKYTIGLGQAKMGFCTDREDINSLCMTVVQNLMERNNLSYDCIGRLEVGTETIIDKSKSVKTNLMQLFEESGNTDIEGIDTTNACYGGTAAVFNAVNWIESSSWDGRYALVVAGDIAVYATGNARPTGGVGAVALLIGPNAPLIFERGLRGTHMQHAYDFYKPDMLSEYPIVDGKLSIQCYLSALDRCYSVYCKKIHAQWQKEGNDKDFTLNDFGFMIFHSPYCKLVQKSLARMLLNDFLNDQNRDKNSIYSGLEAFGDVKLEDTYFDRDVEKAFMKASSELFSQKTKASLLVSNQNGNMYTSSVYGSLASVLAQYSPQQLAGKRIGVFSYGSGLAATLYSLKVTQDATPGSALDKITASLCDLKSRLDSRTGVAPDVFAENMKLREDTHHLVNYIPQGSIDSLFEGTWYLVRVDEKHRRTYARRPTPNDDTLDEGVGLVHSNIATEHIPSPAKKVPRLPATAAEPEAAVISNGEH
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q01581 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S4 | Phosphorylation | Uniprot | |
| K46 | Acetylation | Uniprot | |
| K46 | Ubiquitination | Uniprot | |
| S67 | Phosphorylation | Uniprot | |
| C86 | S-Nitrosylation | Uniprot | |
| T96 | Phosphorylation | Uniprot | |
| K100 | Ubiquitination | Uniprot | |
| S101 | Phosphorylation | Uniprot | |
| K206 | Acetylation | Uniprot | |
| K206 | Ubiquitination | Uniprot | |
| Y213 | Phosphorylation | Uniprot | |
| S221 | Phosphorylation | Uniprot | |
| S227 | Phosphorylation | Uniprot | |
| K239 | Ubiquitination | Uniprot | |
| K246 | Acetylation | Uniprot | |
| K246 | Ubiquitination | Uniprot | |
| K273 | Acetylation | Uniprot | |
| K273 | Ubiquitination | Uniprot | |
| K291 | Ubiquitination | Uniprot | |
| S293 | Phosphorylation | Uniprot | |
| S296 | Phosphorylation | Uniprot | |
| K305 | Sumoylation | Uniprot | |
| K305 | Ubiquitination | Uniprot | |
| Y310 | Phosphorylation | Uniprot | |
| R313 | Methylation | Uniprot | |
| K317 | Acetylation | Uniprot | |
| K317 | Ubiquitination | Uniprot | |
| K321 | Acetylation | Uniprot | |
| K321 | Ubiquitination | Uniprot | |
| K330 | Ubiquitination | Uniprot | |
| S361 | Phosphorylation | Uniprot | |
| K368 | Ubiquitination | Uniprot | |
| K400 | Ubiquitination | Uniprot | |
| K409 | Acetylation | Uniprot | |
| K409 | Ubiquitination | Uniprot | |
| S414 | Phosphorylation | Uniprot | |
| K428 | Ubiquitination | Uniprot | |
| T471 | Phosphorylation | Uniprot | |
| T476 | Phosphorylation | Uniprot | |
| S486 | Phosphorylation | Uniprot | |
| T490 | Phosphorylation | Uniprot | |
| S495 | Phosphorylation | P06493 (CDK1) | Uniprot |
| K498 | Ubiquitination | Uniprot | |
| K499 | Ubiquitination | Uniprot | |
| S516 | Phosphorylation | Uniprot |
研究背景
This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.
Cytoplasm.
Homodimer.
Belongs to the thiolase-like superfamily. HMG-CoA synthase family.
研究领域
· Metabolism > Lipid metabolism > Synthesis and degradation of ketone bodies.
· Metabolism > Amino acid metabolism > Valine, leucine and isoleucine degradation.
· Metabolism > Carbohydrate metabolism > Butanoate metabolism.
· Metabolism > Metabolism of terpenoids and polyketides > Terpenoid backbone biosynthesis.
· Metabolism > Global and overview maps > Metabolic pathways.
限制条款
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