RDH10 Antibody - #DF12105

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产品: RDH10 抗体
货号: DF12105
描述: Rabbit polyclonal antibody to RDH10
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 39 kDa; 38kD(Calculated).
蛋白号: Q8IZV5
RRID: AB_2844910

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
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MS Report
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实验方案
WB Handbook
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%), Xenopus(100%)
克隆:
Polyclonal
特异性:
RDH10 Antibody detects endogenous levels of total RDH10.
RRID:
AB_2844910
引用格式: Affinity Biosciences Cat# DF12105, RRID:AB_2844910.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

RDH 10; RDH10; RDH10_HUMAN; Retinol dehydrogenase 10 (all trans); Retinol dehydrogenase 10; SDR16C4; Short chain dehydrogenase/reductase family 16C member 4;

抗原和靶标

免疫原:
Uniprot:

Q8IZV5(RDH10_HUMAN) >>Visit HPA database.

基因/基因ID:
RDH10(157506)
表达:
Q8IZV5 RDH10_HUMAN:

Detected in retina, kidney, liver, small intestine, placenta, lung, heart and skeletal muscle.

序列:
MNIVVEFFVVTFKVLWAFVLAAARWLVRPKEKSVAGQVCLITGAGSGLGRLFALEFARRRALLVLWDINTQSNEETAGMVRHIYRDLEAADAAALQAGNGEEEILPHCNLQVFTYTCDVGKRENVYLTAERVRKEVGEVSVLVNNAGVVSGHHLLECPDELIERTMMVNCHAHFWTTKAFLPTMLEINHGHIVTVASSLGLFSTAGVEDYCASKFGVVGFHESLSHELKAAEKDGIKTTLVCPYLVDTGMFRGCRIRKEIEPFLPPLKPDYCVKQAMKAILTDQPMICTPRLMYIVTFMKSILPFEAVVCMYRFLGADKCMYPFIAQRKQATNNNEAKNGI

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Chicken
88
Pig
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q8IZV5 作为底物

Site PTM Type Enzyme
K121 Ubiquitination
K134 Ubiquitination
K229 Ubiquitination
T248 Phosphorylation
K268 Ubiquitination
K274 Ubiquitination
Y294 Phosphorylation
T297 Phosphorylation
K319 Ubiquitination
Y322 Phosphorylation
K338 Ubiquitination

研究背景

功能:

Retinol dehydrogenase with a clear preference for NADP. Converts all-trans-retinol to all-trans-retinal. Has no detectable activity towards 11-cis-retinol, 9-cis-retinol and 13-cis-retinol.

细胞定位:

Microsome membrane>Single-pass membrane protein. Endoplasmic reticulum membrane>Single-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Detected in retina, kidney, liver, small intestine, placenta, lung, heart and skeletal muscle.

蛋白家族:

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

研究领域

· Metabolism > Metabolism of cofactors and vitamins > Retinol metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

文献引用

1). Novel Potential Markers of Myofibroblast Differentiation Revealed by Single-Cell RNA Sequencing Analysis of Mesenchymal Stromal Cells in Profibrotic and Adipogenic Conditions. Biomedicines (PubMed: 36979822) [IF=4.7]

Application: IF/ICC    Species: Human    Sample: adipose tissue

Figure 4. Immunocytochemical evaluation of CHD3 (green), RDH10 (green), NTM (green), and αSMA (red) in the control (Control) MSCs and after incubation in profibrotic (F) or adipogenic conditions (Ad). Fluorescent microscopy, blue staining—nuclei stained with DAPI, scale bar is 50 µm (A). The percentage of CHD3-positive cells, mean ± SE, p value is indicated (B). The percentage of cells with neurotrimin clusters, mean ± SE, p value is indicated (C).

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