CLTA Antibody - #DF12148
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12148, RRID:AB_2844953.
展开/折叠
Clathrin light chain A; Clathrin light chain B; Clathrin light chain LCA; Clathrin light chain LCB; Clathrin light polypeptide A; Clathrin light polypeptide; Clathrin light polypeptide B; Clathrin light polypeptide LCA; Clathrin light polypeptide LCB; Clathrin, light chain (Lcb); Clathrin, light polypeptide (Lca); Clathrin, light polypeptide (Lcb); CLCA_HUMAN; CLTA; CLTB; Lca; LCB;
抗原和靶标
- P09496 CLCA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAELDPFGAPAGAPGGPALGNGVAGAGEEDPAAAFLAQQESEIAGIENDEAFAILDGGAPGPQPHGEPPGGPDAVDGVMNGEYYQESNGPTDSYAAISQVDRLQSEPESIRKWREEQMERLEALDANSRKQEAEWKEKAIKELEEWYARQDEQLQKTKANNRVADEAFYKQPFADVIGYVTNINHPCYSLEQAAEEAFVNDIDESSPGTEWERVARLCDFNPKSSKQAKDVSRMRSVLISLKQAPLVH
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P09496 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y83 | Phosphorylation | Uniprot | |
| Y84 | Phosphorylation | Uniprot | |
| S87 | Phosphorylation | Uniprot | |
| T91 | Phosphorylation | Uniprot | |
| S93 | Phosphorylation | Uniprot | |
| Y94 | Phosphorylation | Uniprot | |
| S105 | Phosphorylation | Uniprot | |
| S109 | Phosphorylation | Uniprot | |
| S128 | Phosphorylation | Uniprot | |
| K130 | Ubiquitination | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| Y147 | Phosphorylation | Uniprot | |
| K156 | Ubiquitination | Uniprot | |
| S206 | Phosphorylation | Uniprot | |
| K223 | Methylation | Uniprot | |
| K223 | Ubiquitination | Uniprot | |
| K226 | Methylation | Uniprot | |
| S236 | Phosphorylation | Uniprot | |
| K242 | Acetylation | Uniprot | |
| K242 | Ubiquitination | Uniprot |
研究背景
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge.
Cytoplasmic vesicle membrane>Peripheral membrane protein>Cytoplasmic side. Membrane>Coated pit>Peripheral membrane protein>Cytoplasmic side. Cytoplasm>Cytoskeleton>Spindle.
Note: Cytoplasmic face of coated pits and vesicles. In complex with TACC3 and CKAP5 (forming the TACC3/ch-TOG/clathrin complex) localized to inter-microtubule bridges in mitotic spindles.
Clathrin coats are formed from molecules containing 3 heavy chains and 3 light chains. Interacts with CALY; the interaction stimulates clathrin self-assembly and clathrin-mediated endocytosis. Interacts with CKAP5 and TACC3 forming the TACC3/ch-TOG/clathrin complex located at spindle inter-microtubules bridges; the complex implicates clathrin triskelions.
Belongs to the clathrin light chain family.
研究领域
· Cellular Processes > Transport and catabolism > Lysosome. (View pathway)
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Human Diseases > Neurodegenerative diseases > Huntington's disease.
· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.
· Organismal Systems > Nervous system > Synaptic vesicle cycle.
· Organismal Systems > Excretory system > Endocrine and other factor-regulated calcium reabsorption.
限制条款
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