产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12188, RRID:AB_2844993.
展开/折叠
Adaptor protein complex AP-1 mu-1 subunit; Adaptor-related protein complex 1 mu-1 subunit; AP-1 complex subunit mu-1; AP-mu chain family member mu1A; Ap1m1; AP1M1_HUMAN; AP47; CLAPM2; Clathrin adaptor protein AP47; Clathrin assembly protein complex 1 medium chain 1; Clathrin assembly protein complex 1 medium chain; Clathrin assembly protein complex AP1 mu subunit; Clathrin coat assembly protein AP47; Clathrin coat-associated protein AP47; CLTNM; Golgi adaptor AP 1 47 kDa protein; Golgi adaptor HA1/AP1 adaptin mu-1 subunit; HA1 47 kDa subunit; MU 1A; Mu-adaptin 1; Mu1A-adaptin;
抗原和靶标
- Q9BXS5 AP1M1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSASAVYVLDLKGKVLICRNYRGDVDMSEVEHFMPILMEKEEEGMLSPILAHGGVRFMWIKHNNLYLVATSKKNACVSLVFSFLYKVVQVFSEYFKELEEESIRDNFVIIYELLDELMDFGYPQTTDSKILQEYITQEGHKLETGAPRPPATVTNAVSWRSEGIKYRKNEVFLDVIESVNLLVSANGNVLRSEIVGSIKMRVFLSGMPELRLGLNDKVLFDNTGRGKSKSVELEDVKFHQCVRLSRFENDRTISFIPPDGEFELMSYRLNTHVKPLIWIESVIEKHSHSRIEYMIKAKSQFKRRSTANNVEIHIPVPNDADSPKFKTTVGSVKWVPENSEIVWSIKSFPGGKEYLMRAHFGLPSVEAEDKEGKPPISVKFEIPYFTTSGIQVRYLKIIEKSGYQALPWVRYITQNGDYQLRTQ
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9BXS5 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| K14 | Ubiquitination | Uniprot | |
| S28 | Phosphorylation | Uniprot | |
| S47 | Phosphorylation | Uniprot | |
| K72 | Ubiquitination | Uniprot | |
| Y134 | Phosphorylation | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| T144 | Phosphorylation | Q2M2I8 (AAK1) | Uniprot |
| T152 | Phosphorylation | Uniprot | |
| T154 | Phosphorylation | Uniprot | |
| S158 | Phosphorylation | Uniprot | |
| K165 | Ubiquitination | Uniprot | |
| K199 | Ubiquitination | Uniprot | |
| K217 | Ubiquitination | Uniprot | |
| T223 | Phosphorylation | Uniprot | |
| R225 | Methylation | Uniprot | |
| K227 | Ubiquitination | Uniprot | |
| K229 | Ubiquitination | Uniprot | |
| K237 | Ubiquitination | Uniprot | |
| S299 | Phosphorylation | Uniprot | |
| S305 | Phosphorylation | Uniprot | |
| S322 | Phosphorylation | Uniprot | |
| K324 | Ubiquitination | Uniprot | |
| K326 | Methylation | Uniprot | |
| K326 | Ubiquitination | Uniprot | |
| S331 | Phosphorylation | Uniprot | |
| K333 | Ubiquitination | Uniprot | |
| K346 | Ubiquitination | Uniprot | |
| K370 | Ubiquitination | Uniprot | |
| K396 | Acetylation | Uniprot | |
| K396 | Ubiquitination | Uniprot | |
| K400 | Ubiquitination | Uniprot | |
| R421 | Methylation | Uniprot |
研究背景
Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
Phosphorylation of membrane-bound AP1M1/AP1M2 increases its affinity for sorting signals.
Golgi apparatus. Cytoplasmic vesicle>Clathrin-coated vesicle membrane>Peripheral membrane protein>Cytoplasmic side.
Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.
Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3). Interacts with MARCHF11 (By similarity). Associates with the AP1(MU)-Nef-MHC-I complex; this complex is required for MHC-I internalization.
(Microbial infection) Interacts with HIV-1 Nef.
Belongs to the adaptor complexes medium subunit family.
研究领域
· Cellular Processes > Transport and catabolism > Lysosome. (View pathway)
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