产品: P4HA2 抗体
货号: DF12440
描述: Rabbit polyclonal antibody to P4HA2
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Horse, Sheep, Rabbit, Dog
分子量: 61 kDa; 61kD(Calculated).
蛋白号: O15460
RRID: AB_2845245

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Horse(100%), Sheep(100%), Rabbit(83%), Dog(100%)
克隆:
Polyclonal
特异性:
P4HA2 Antibody detects endogenous levels of total P4HA2.
RRID:
AB_2845245
引用格式: Affinity Biosciences Cat# DF12440, RRID:AB_2845245.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

2-oxoglutarate-4-dioxygenase subunit alpha-2; 4 PH alpha 2; 4-PH alpha-2; C-P4Halpha(II); collagen prolyl 4-hydroxylase alpha(II); P4HA2; P4HA2_HUMAN; procollagen proline, 2 oxoglutarate 4 dioxygenase (proline 4 hydroxylase), alpha polypeptide II; procollagen proline, 2 oxoglutarate 4 dioxygenase alpha subunit, isoform 2; Procollagen-proline; procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2; Prolyl 4 hydroxylase alpha 2 subunit; Prolyl 4 hydroxylase subunit alpha 2 [Precursor]; Prolyl 4-hydroxylase subunit alpha-2; Prolyl 4-hydroxylase, alpha polypeptide II;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
序列:
MKLWVSALLMAWFGVLSCVQAEFFTSIGHMTDLIYAEKELVQSLKEYILVEEAKLSKIKSWANKMEALTSKSAADAEGYLAHPVNAYKLVKRLNTDWPALEDLVLQDSAAGFIANLSVQRQFFPTDEDEIGAAKALMRLQDTYRLDPGTISRGELPGTKYQAMLSVDDCFGMGRSAYNEGDYYHTVLWMEQVLKQLDAGEEATTTKSQVLDYLSYAVFQLGDLHRALELTRRLLSLDPSHERAGGNLRYFEQLLEEEREKTLTNQTEAELATPEGIYERPVDYLPERDVYESLCRGEGVKLTPRRQKRLFCRYHHGNRAPQLLIAPFKEEDEWDSPHIVRYYDVMSDEEIERIKEIAKPKLARATVRDPKTGVLTVASYRVSKSSWLEEDDDPVVARVNRRMQHITGLTVKTAELLQVANYGVGGQYEPHFDFSRNDERDTFKHLGTGNRVATFLNYMSDVEAGGATVFPDLGAAIWPKKGTAVFWYNLLRSGEGDYRTRHAACPVLVGCKWVSNKWFHERGQEFLRPCGSTEVD

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Rabbit
83
Chicken
75
Xenopus
70
Zebrafish
67
Bovine
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O15460 作为底物

Site PTM Type Enzyme
T31 Phosphorylation
Y35 Phosphorylation
S43 Phosphorylation
K45 Ubiquitination
K54 Ubiquitination
K59 Ubiquitination
K64 Ubiquitination
K71 Ubiquitination
Y79 Phosphorylation
K88 Ubiquitination
K134 Ubiquitination
K194 Ubiquitination
K206 Ubiquitination
K300 Ubiquitination
K328 Sumoylation
K328 Ubiquitination
S346 Phosphorylation
K383 Ubiquitination
Y421 Phosphorylation
T441 Phosphorylation
K443 Ubiquitination
T447 Phosphorylation
T482 Phosphorylation
Y487 Phosphorylation

研究背景

功能:

Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.

细胞定位:

Endoplasmic reticulum lumen.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Heterotetramer of two alpha-2 chains and two beta chains (the beta chain is the multi-functional PDI).

蛋白家族:

Belongs to the P4HA family.

研究领域

· Metabolism > Amino acid metabolism > Arginine and proline metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

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