PAFAH2 Antibody - #DF12442

(3)  
产品: PAFAH2 抗体
货号: DF12442
描述: Rabbit polyclonal antibody to PAFAH2
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Horse, Dog
分子量: 44-46 kDa; 44kD(Calculated).
蛋白号: Q99487
RRID: AB_2845247

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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实验方案
WB Handbook
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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(89%), Horse(100%), Dog(89%)
克隆:
Polyclonal
特异性:
PAFAH2 Antibody detects endogenous levels of total PAFAH2.
RRID:
AB_2845247
引用格式: Affinity Biosciences Cat# DF12442, RRID:AB_2845247.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

EC 3.1.1.47; HSD PLA2; HSDPLA2; Platelet activating factor acetylhydrolase 2; Platelet activating factor acetylhydrolase 2 cytoplasmic; platelet-activating factor acetylhydrolase 2, 40kDa; SD PLA2; Serine dependent phospholipase A2;

抗原和靶标

免疫原:
Uniprot:

Q99487(PAFA2_HUMAN) >>Visit HPA database.

基因/基因ID:
PAFAH2(5051)
表达:
Q99487 PAFA2_HUMAN:

Broadly expressed in different tissues, but high in B- and T-lymphocytes. In brain, expression is restricted to amygdala and frontal cortex.

序列:
MGVNQSVGFPPVTGPHLVGCGDVMEGQNLQGSFFRLFYPCQKAEETMEQPLWIPRYEYCTGLAEYLQFNKRCGGLLFNLAVGSCRLPVSWNGPFKTKDSGYPLIIFSHGLGAFRTLYSAFCMELASRGFVVAVPEHRDRSAATTYFCKQAPEENQPTNESLQEEWIPFRRVEEGEKEFHVRNPQVHQRVSECLRVLKILQEVTAGQTVFNILPGGLDLMTLKGNIDMSRVAVMGHSFGGATAILALAKETQFRCAVALDAWMFPLERDFYPKARGPVFFINTEKFQTMESVNLMKKICAQHEQSRIITVLGSVHRSQTDFAFVTGNLIGKFFSTETRGSLDPYEGQEVMVRAMLAFLQKHLDLKEDYNQWNNLIEGIGPSLTPGAPHHLSSL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Pig
89
Dog
89
Bovine
78
Sheep
78
Rabbit
78
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q99487 作为底物

Site PTM Type Enzyme
Y101 Phosphorylation
K148 Ubiquitination
K176 Acetylation
K176 Ubiquitination
K330 Ubiquitination
K364 Ubiquitination

研究背景

功能:

Has a marked selectivity for phospholipids with short acyl chains at the sn-2 position. May share a common physiologic function with the plasma-type enzyme.

细胞定位:

Cytoplasm. Membrane>Lipid-anchor. Endoplasmic reticulum membrane>Lipid-anchor.
Note: In resting cells, localizes to intracellular membranes and cytoplasm. Translocates from the cytoplasm to intracellular membranes upon oxidative stress.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Broadly expressed in different tissues, but high in B- and T-lymphocytes. In brain, expression is restricted to amygdala and frontal cortex.

亚基结构:

Monomer.

蛋白家族:

Belongs to the serine esterase family.

研究领域

· Metabolism > Lipid metabolism > Ether lipid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

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