ATP6V1E1 Antibody - #DF12565

(2)  
产品: ATP6V1E1 抗体
货号: DF12565
描述: Rabbit polyclonal antibody to ATP6V1E1
应用: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 31 kDa; 26kD(Calculated).
蛋白号: P36543
RRID: AB_2845527

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 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(100%), Chicken(91%), Xenopus(82%)
克隆:
Polyclonal
特异性:
ATP6V1E1 Antibody detects endogenous levels of total ATP6V1E1.
RRID:
AB_2845527
引用格式: Affinity Biosciences Cat# DF12565, RRID:AB_2845527.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

抗原和靶标

免疫原:
Uniprot:

P36543(VATE1_HUMAN) >>Visit HPA database.

基因/基因ID:
ATP6V1E1(529)
表达:
P36543 VATE1_HUMAN:

Ubiquitous (PubMed:12036578). High expression in the skin (PubMed:28065471).

序列:
MALSDADVQKQIKHMMAFIEQEANEKAEEIDAKAEEEFNIEKGRLVQTQRLKIMEYYEKKEKQIEQQKKIQMSNLMNQARLKVLRARDDLITDLLNEAKQRLSKVVKDTTRYQVLLDGLVLQGLYQLLEPRMIVRCRKQDFPLVKAAVQKAIPMYKIATKNDVDVQIDQESYLPEDIAGGVEIYNGDRKIKVSNTLESRLDLIAQQMMPEVRGALFGANANRKFLD

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
100
Sheep
92
Chicken
91
Xenopus
82
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P36543 作为底物

Site PTM Type Enzyme
A2 Acetylation
S4 Phosphorylation
K10 Ubiquitination
K42 Ubiquitination
K52 Acetylation
Y56 Phosphorylation
Y57 Phosphorylation
K59 Ubiquitination
K69 Ubiquitination
K99 Ubiquitination
S103 Phosphorylation
K138 Ubiquitination
K145 Ubiquitination
K156 Ubiquitination
K191 Acetylation
K191 Ubiquitination

研究背景

功能:

Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.

组织特异性:

Ubiquitous. High expression in the skin.

亚基结构:

V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'' and d). Interacts with RABL2/RABL2A; binds preferentially to GTP-bound RABL2 (By similarity). Interacts with ALDOC. Interacts with RAB11B.

蛋白家族:

Belongs to the V-ATPase E subunit family.

研究领域

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Vibrio cholerae infection.

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Metabolism > Energy metabolism > Oxidative phosphorylation.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Nervous system > Synaptic vesicle cycle.

· Organismal Systems > Excretory system > Collecting duct acid secretion.

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