CNOT3 Antibody - #DF12583

(3)  
产品: CNOT3 抗体
货号: DF12583
描述: Rabbit polyclonal antibody to CNOT3
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Rabbit, Dog, Xenopus
分子量: 120 kDa; 82kD(Calculated).
蛋白号: O75175
RRID: AB_2845545

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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实验方案
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
CNOT3 Antibody detects endogenous levels of total CNOT3.
RRID:
AB_2845545
引用格式: Affinity Biosciences Cat# DF12583, RRID:AB_2845545.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CCR4 associated factor 3; CCR4-associated factor 3; CCR4-NOT transcription complex subunit 3; CNOT3; CNOT3_HUMAN; KIAA0691; LENG2; Leukocyte receptor cluster member 2; negative regulator of transcription 3, S. cerevisiae, homolog of; NOT3 (negative regulator of transcription 3, yeast) homolog; NOT3; NOT3H;

抗原和靶标

免疫原:
Uniprot:

O75175(CNOT3_HUMAN) >>Visit HPA database.

基因/基因ID:
CNOT3(4849)
表达:
O75175 CNOT3_HUMAN:

Ubiquitous. Highly expressed in brain, heart, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes.

序列:
MADKRKLQGEIDRCLKKVSEGVEQFEDIWQKLHNAANANQKEKYEADLKKEIKKLQRLRDQIKTWVASNEIKDKRQLIDNRKLIETQMERFKVVERETKTKAYSKEGLGLAQKVDPAQKEKEEVGQWLTNTIDTLNMQVDQFESEVESLSVQTRKKKGDKDKQDRIEGLKRHIEKHRYHVRMLETILRMLDNDSILVDAIRKIKDDVEYYVDSSQDPDFEENEFLYDDLDLEDIPQALVATSPPSHSHMEDEIFNQSSSTPTSTTSSSPIPPSPANCTTENSEDDKKRGRSTDSEVSQSPAKNGSKPVHSNQHPQSPAVPPTYPSGPPPAASALSTTPGNNGVPAPAAPPSALGPKASPAPSHNSGTPAPYAQAVAPPAPSGPSTTQPRPPSVQPSGGGGGGSGGGGSSSSSNSSAGGGAGKQNGATSYSSVVADSPAEVALSSSGGNNASSQALGPPSGPHNPPPSTSKEPSAAAPTGAGGVAPGSGNNSGGPSLLVPLPVNPPSSPTPSFSDAKAAGALLNGPPQFSTAPEIKAPEPLSSLKSMAERAAISSGIEDPVPTLHLTERDIILSSTSAPPASAQPPLQLSEVNIPLSLGVCPLGPVPLTKEQLYQQAMEEAAWHHMPHPSDSERIRQYLPRNPCPTPPYHHQMPPPHSDTVEFYQRLSTETLFFIFYYLEGTKAQYLAAKALKKQSWRFHTKYMMWFQRHEEPKTITDEFEQGTYIYFDYEKWGQRKKEGFTFEYRYLEDRDLQ

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Xenopus
100
Rabbit
100
Sheep
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O75175 作为底物

Site PTM Type Enzyme
K41 Ubiquitination
K82 Ubiquitination
T100 Phosphorylation
S104 Phosphorylation
K105 Ubiquitination
S194 Phosphorylation
S245 Phosphorylation
S291 Phosphorylation
T292 Phosphorylation
S294 Phosphorylation
S297 Phosphorylation
S299 Phosphorylation
S467 Phosphorylation
T468 Phosphorylation
S469 Phosphorylation
S506 Phosphorylation
S507 Phosphorylation
S541 Phosphorylation
S542 Phosphorylation
R635 Methylation
T645 Phosphorylation
Y685 Phosphorylation
K689 Ubiquitination
K737 Ubiquitination

研究背景

功能:

Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. May be involved in metabolic regulation; may be involved in recruitment of the CCR4-NOT complex to deadenylation target mRNAs involved in energy metabolism. Involved in mitotic progression and regulation of the spindle assembly checkpoint by regulating the stability of MAD1L1 mRNA. Can repress transcription and may link the CCR4-NOT complex to transcriptional regulation; the repressive function may involve histone deacetylases. Involved in the maintenance of embryonic stem (ES) cell identity.

细胞定位:

Cytoplasm. Nucleus. Cytoplasm>P-body.
Note: NANOS2 promotes its localization to P-body.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitous. Highly expressed in brain, heart, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes.

亚基结构:

Component of the CCR4-NOT complex; distinct complexes seem to exist that differ in the participation of probably mutually exclusive catalytic subunits. In the complex interacts directly with CNOT2. Interacts with TIP120B and NANOS2.

蛋白家族:

Belongs to the CNOT2/3/5 family.

研究领域

· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.

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