Cathepsin L Antibody - #DF12880

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产品: Cathepsin L 抗体
货号: DF12880
描述: Rabbit polyclonal antibody to Cathepsin L
应用: WB IF/ICC
反应: Human, Mouse
分子量: 30 kDa; 38kD(Calculated).
蛋白号: P07711
RRID: AB_2845841

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
克隆:
Polyclonal
特异性:
Cathepsin L Antibody detects endogenous levels of total Cathepsin L.
RRID:
AB_2845841
引用格式: Affinity Biosciences Cat# DF12880, RRID:AB_2845841.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Cathepsin L; cathepsin L, 1 b; Cathepsin L1; Cathepsin L1 light chain; CathepsinL; CATL; CATL1_HUMAN; cb15; CTSL; CTSL1; ctsl1b; FLJ31037; hgg1; Major excreted protein; MEP; MGC123162; wu:fb30g09;

抗原和靶标

免疫原:
Uniprot:

P07711(CATL1_HUMAN) >>Visit HPA database.

基因/基因ID:
CTSL(1514)
序列:
MNPTLILAAFCLGIASATLTFDHSLEAQWTKWKAMHNRLYGMNEEGWRRAVWEKNMKMIELHNQEYREGKHSFTMAMNAFGDMTSEEFRQVMNGFQNRKPRKGKVFQEPLFYEAPRSVDWREKGYVTPVKNQGQCGSCWAFSATGALEGQMFRKTGRLISLSEQNLVDCSGPQGNEGCNGGLMDYAFQYVQDNGGLDSEESYPYEATEESCKYNPKYSVANDTGFVDIPKQEKALMKAVATVGPISVAIDAGHESFLFYKEGIYFEPDCSSEDMDHGVLVVGYGFESTESDNNKYWLVKNSWGEEWGMGGYVKMAKDRRNHCGIASAASYPTV

翻译修饰 - P07711 作为底物

Site PTM Type Enzyme
K104 Sumoylation
K104 Ubiquitination
K123 Ubiquitination
K216 Ubiquitination
N221 N-Glycosylation
T223 Phosphorylation
K230 Ubiquitination
Y295 Phosphorylation
Y311 Phosphorylation
C322 S-Nitrosylation

研究背景

功能:

Thiol protease important for the overall degradation of proteins in lysosomes (Probable). Involved in the solubilization of cross-linked TG/thyroglobulin and in the subsequent release of thyroid hormone thyroxine (T4) by limited proteolysis of TG/thyroglobulin in the thyroid follicle lumen (By similarity).

细胞定位:

Lysosome. Apical cell membrane>Peripheral membrane protein>Extracellular side.
Note: Localizes to the apical membrane of thyroid epithelial cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Dimer of a heavy and a light chain linked by disulfide bonds.

蛋白家族:

Belongs to the peptidase C1 family.

研究领域

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Cellular Processes > Transport and catabolism > Lysosome.   (View pathway)

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Organismal Systems > Immune system > Antigen processing and presentation.   (View pathway)

文献引用

1). TFE3 regulates the function of the autophagy-lysosome pathway to drive the invasion and metastasis of papillary thyroid carcinoma. Analytical Cellular Pathology (PubMed: 34660181) [IF=3.2]

Application: WB    Species: Human    Sample: PTC cells

Figure 5 TFE3 induces autophagy-lysosome in PTC cells. (a, b) After transfection with pcDNA3.1-TFE3 or si-TFE3, BCPAP and KTC-1 cells showed significant changes in TFE3 protein levels. Overexpression of TFE3 increased the expression of P62/SQSTM1, and the opposite results were observed in BCPAP and KTC-1 cells transfected with si-TFE3. The ratio of LC3II/LC3Iin BCPAP and KTC-1 cells increased significantly after pcDNA3.1-TFE3 transfection, and the opposite results appeared after si-TFE3 transfection. (c) After knocking down TFE3, the expression of LC3 and P62/SQSTM1 in PTC cells was decreased, and overexpression of TFE3 increased the expression of LC3 and P62/SQSTM1 in PTC cells (∗P < 0.05). (d) After knocking down TFE3, the expression of CTSB and CTSL in PTC cells was decreased, while TFE3 is overexpression, the expression of CTSB and CTSL in PTC cells increased (∗P < 0.05).

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