产品: 磷酸化 IL-10R alpha (Tyr496) 抗体
货号: AF3435
描述: Rabbit polyclonal antibody to Phospho-IL-10R alpha (Tyr496)
应用: WB IF/ICC
反应: Human
预测: Pig, Horse, Rabbit, Dog, Chicken
分子量: 63kDa; 63kD(Calculated).
蛋白号: Q13651
RRID: AB_2834877

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
预测:
Pig(100%), Horse(89%), Rabbit(89%), Dog(100%), Chicken(88%)
克隆:
Polyclonal
特异性:
Phospho-IL-10R alpha (Tyr496) Antibody detects endogenous levels of IL-10R alpha only when phosphorylated at Tyrosine 496.
RRID:
AB_2834877
引用格式: Affinity Biosciences Cat# AF3435, RRID:AB_2834877.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CD210; CDw210a; CDw210a antigen; HIL 10R; HIL10R; I10R1_HUMAN; IL 10R 1; IL 10R A; IL 10R; IL 10R1; IL-10 receptor subunit alpha; IL-10R subunit 1; IL-10R subunit alpha; IL-10R1; IL-10RA; IL10R; IL10R1; IL10RA; Interleukin 10 receptor alpha; Interleukin 10 receptor alpha chain; Interleukin-10 receptor subunit 1; Interleukin-10 receptor subunit alpha;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q13651 I10R1_HUMAN:

Primarily expressed in hematopoetic cells including B-cells, T-cells, NK cells, monocytes and macrophages. Not expressed in non-hematopoetic cells such as fibroblasts or endothelial cells.

描述:
The protein encoded by this gene is a receptor for interleukin 10. This protein is structurally related to interferon receptors. It has been shown to mediate the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines.
序列:
MLPCLVVLLAALLSLRLGSDAHGTELPSPPSVWFEAEFFHHILHWTPIPNQSESTCYEVALLRYGIESWNSISNCSQTLSYDLTAVTLDLYHSNGYRARVRAVDGSRHSNWTVTNTRFSVDEVTLTVGSVNLEIHNGFILGKIQLPRPKMAPANDTYESIFSHFREYEIAIRKVPGNFTFTHKKVKHENFSLLTSGEVGEFCVQVKPSVASRSNKGMWSKEECISLTRQYFTVTNVIIFFAFVLLLSGALAYCLALQLYVRRRKKLPSVLLFKKPSPFIFISQRPSPETQDTIHPLDEEAFLKVSPELKNLDLHGSTDSGFGSTKPSLQTEEPQFLLPDPHPQADRTLGNREPPVLGDSCSSGSSNSTDSGICLQEPSLSPSTGPTWEQQVGSNSRGQDDSGIDLVQNSEGRAGDTQGGSALGHHSPPEPEVPGEEDPAAVAFQGYLRQTRCAEEKATKTGCLEEESPLTDGLGPKFGRCLVDEAGLHPPALAKGYLKQDPLEMTLASSGAPTGQWNQPTEEWSLLALSSCSDLGISDWSFAHDLAPLGCVAAPGGLLGSFNSDLVTLPLISSLQSSE

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Dog
100
Horse
89
Rabbit
89
Chicken
88
Bovine
78
Sheep
78
Xenopus
71
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q13651 作为底物

Site PTM Type Enzyme
K183 Acetylation
S268 Phosphorylation
S319 Phosphorylation
S323 Phosphorylation
S370 Phosphorylation
Y446 Phosphorylation P23458 (JAK1) , P29597 (TYK2)
Y496 Phosphorylation P29597 (TYK2) , P23458 (JAK1)

研究背景

功能:

Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Upon binding to IL10, induces a conformational change in IL10RB, allowing IL10RB to bind IL10 as well. In turn, the heterotetrameric assembly complex, composed of two subunits of IL10RA and IL10RB, activates the kinases JAK1 and TYK2 that are constitutively associated with IL10RA and IL10RB respectively. These kinases then phosphorylate specific tyrosine residues in the intracellular domain in IL10RA leading to the recruitment and subsequent phosphorylation of STAT3. Once phosphorylated, STAT3 homodimerizes, translocates to the nucleus and activates the expression of anti-inflammatory genes. In addition, IL10RA-mediated activation of STAT3 inhibits starvation-induced autophagy.

翻译修饰:

Phosphorylated. Phosphorylation of the cytoplasmic tail induced STAT3 activation.

Ubiquitinated by BTRC; ubiquitination leads to endocytosis and subsequent degradation of IL10RA.

细胞定位:

Cell membrane>Single-pass type I membrane protein. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Primarily expressed in hematopoetic cells including B-cells, T-cells, NK cells, monocytes and macrophages. Not expressed in non-hematopoetic cells such as fibroblasts or endothelial cells.

亚基结构:

Interacts with IL10. Interacts with IL10RB. Interacts (via its cytoplasmic domain) with JAK1 (via N-terminus). Interacts with BTRC; this interaction leads to IL10RA ubiquitination and subsequent degradation. Interacts with STAT3 (By similarity).

(Microbial infection) Interacts with human cytomegalovirus protein IL10.

(Microbial infection) Interacts with Epstein-Barr virus protein IL10.

蛋白家族:

Belongs to the type II cytokine receptor family.

研究领域

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

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