DDX6 Antibody - #DF12937

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产品: DDX6 抗体
货号: DF12937
描述: Rabbit polyclonal antibody to DDX6
应用: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 54 kDa; 54kD(Calculated).
蛋白号: P26196
RRID: AB_2845898

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(92%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(92%)
克隆:
Polyclonal
特异性:
DDX6 Antibody detects endogenous levels of total DDX6.
RRID:
AB_2845898
引用格式: Affinity Biosciences Cat# DF12937, RRID:AB_2845898.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ATP dependent RNA helicase DDX6; ATP dependent RNA helicase p54; DDX6; DEAD (Asp Glu Ala Asp) box polypeptide 6; DEAD box 6; DEAD box protein 6; DEAD/H (Asp Glu Ala Asp/His) box polypeptide 6 (RNA helicase 54kD); FLJ36338; HLR2; Oncogene RCK; P54; Probable ATP-dependent RNA helicase DDX6; RCK;

抗原和靶标

免疫原:
Uniprot:

P26196(DDX6_HUMAN) >>Visit HPA database.

基因/基因ID:
DDX6(1656)
表达:
P26196 DDX6_HUMAN:

Abundantly expressed in most tissues.

序列:
MSTARTENPVIMGLSSQNGQLRGPVKPTGGPGGGGTQTQQQMNQLKNTNTINNGTQQQAQSMTTTIKPGDDWKKTLKLPPKDLRIKTSDVTSTKGNEFEDYCLKRELLMGIFEMGWEKPSPIQEESIPIALSGRDILARAKNGTGKSGAYLIPLLERLDLKKDNIQAMVIVPTRELALQVSQICIQVSKHMGGAKVMATTGGTNLRDDIMRLDDTVHVVIATPGRILDLIKKGVAKVDHVQMIVLDEADKLLSQDFVQIMEDIILTLPKNRQILLYSATFPLSVQKFMNSHLQKPYEINLMEELTLKGVTQYYAYVTERQKVHCLNTLFSRLQINQSIIFCNSSQRVELLAKKISQLGYSCFYIHAKMRQEHRNRVFHDFRNGLCRNLVCTDLFTRGIDIQAVNVVINFDFPKLAETYLHRIGRSGRFGHLGLAINLITYDDRFNLKSIEEQLGTEIKPIPSNIDKSLYVAEYHSEPVEDEKP

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
92
Zebrafish
92
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P26196 作为底物

Site PTM Type Enzyme
S15 Phosphorylation
S16 Phosphorylation
K26 Ubiquitination
T36 Phosphorylation
T38 Phosphorylation
T64 Phosphorylation
K67 Acetylation
K67 Ubiquitination
K73 Acetylation
K73 Ubiquitination
K74 Acetylation
T75 Phosphorylation
K81 Ubiquitination
K86 Ubiquitination
T87 Phosphorylation
K94 Ubiquitination
K104 Acetylation
K104 Ubiquitination
K146 Ubiquitination
S147 Phosphorylation
K162 Ubiquitination
K195 Ubiquitination
T199 Phosphorylation
K231 Ubiquitination
Y312 Phosphorylation
Y313 Phosphorylation
C324 S-Nitrosylation
T327 Phosphorylation
S330 Phosphorylation
R375 Methylation
T439 Phosphorylation
Y440 Phosphorylation
K447 Ubiquitination
K458 Ubiquitination
S462 Phosphorylation
K466 Ubiquitination
Y473 Phosphorylation
S475 Phosphorylation
K482 Ubiquitination

研究背景

功能:

In the process of mRNA degradation, plays a role in mRNA decapping. Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts.

翻译修饰:

Sumoylated.

细胞定位:

Cytoplasm>P-body. Cytoplasm. Nucleus.
Note: Upon cellular stress, relocalizes to stress granules (PubMed:26184334).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Abundantly expressed in most tissues.

亚基结构:

Forms a complex with DCP1A, DCP2, EDC3 and EDC4/HEDLS. Interacts with LIMD1, WTIP and AJUBA. Interacts with APOBEC3G in an RNA-dependent manner. Interacts with RC3H1 (By similarity). Interacts with ATXN2L. Interacts with MCRIP1. Interacts with MCRIP2. Interacts with NUFIP2. Interacts with TRIM71 (via NHL repeats) in an RNA-dependent manner.

蛋白家族:

Belongs to the DEAD box helicase family. DDX6/DHH1 subfamily.

研究领域

· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.

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