MTHFD1 Antibody - #DF13159

(1)  
产品: MTHFD1 抗体
货号: DF13159
描述: Rabbit polyclonal antibody to MTHFD1
应用: WB
反应: Human, Mouse
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 101 kDa; 102kD(Calculated).
蛋白号: P11586
RRID: AB_2846119

浏览相似产品>>

   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(100%), Bovine(91%), Horse(82%), Sheep(91%), Rabbit(100%), Dog(100%), Chicken(82%), Xenopus(82%)
克隆:
Polyclonal
特异性:
MTHFD1 Antibody detects endogenous levels of total MTHFD1.
RRID:
AB_2846119
引用格式: Affinity Biosciences Cat# DF13159, RRID:AB_2846119.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

5,10 methylenetetrahydrofolate dehydrogenase 5,10 methylenetetrahydrofolate cyclohydrolase,10 formyltetrahydrofolate synthetase; C 1 tetrahydrofolate synthase cytoplasmic; C1 tetrahydrofolate synthase cytoplasmic; C1 THF synthase; C1-THF synthase; C1TC; C1TC_HUMAN; Cytoplasmic C 1 tetrahydrofolate synthase; Formyltetrahydrofolate synthetase; Methenyltetrahydrofolate cyclohydrolase; Methylenetetrahydrofolate dehydrogenase (NADP+ dependent) methenyltetrahydrofolate cyclohydrolase formyltetrahydrofolate synthetase; Methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 methenyltetrahydrofolate cyclohydrolase formyltetrahydrofolate synthetase; Methylenetetrahydrofolate dehydrogenase; MTHFC; MTHFD; MTHFD1; NADP dependent cyclohydrolase formyltetrahydrofolate synthetase; OTTHUMP00000180657;

抗原和靶标

免疫原:
Uniprot:

P11586(C1TC_HUMAN) >>Visit HPA database.

基因/基因ID:
MTHFD1(4522)
表达:
P11586 C1TC_HUMAN:

Ubiquitous.

序列:
MAPAEILNGKEISAQIRARLKNQVTQLKEQVPGFTPRLAILQVGNRDDSNLYINVKLKAAEEIGIKATHIKLPRTTTESEVMKYITSLNEDSTVHGFLVQLPLDSENSINTEEVINAIAPEKDVDGLTSINAGRLARGDLNDCFIPCTPKGCLELIKETGVPIAGRHAVVVGRSKIVGAPMHDLLLWNNATVTTCHSKTAHLDEEVNKGDILVVATGQPEMVKGEWIKPGAIVIDCGINYVPDDKKPNGRKVVGDVAYDEAKERASFITPVPGGVGPMTVAMLMQSTVESAKRFLEKFKPGKWMIQYNNLNLKTPVPSDIDISRSCKPKPIGKLAREIGLLSEEVELYGETKAKVLLSALERLKHRPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGAHLYQNVFACVRQPSQGPTFGIKGGAAGGGYSQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARIFHELTQTDKALFNRLVPSVNGVRRFSDIQIRRLKRLGIEKTDPTTLTDEEINRFARLDIDPETITWQRVLDTNDRFLRKITIGQAPTEKGHTRTAQFDISVASEIMAVLALTTSLEDMRERLGKMVVASSKKGEPVSAEDLGVSGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSIIADRIALKLVGPEGFVVTEAGFGADIGMEKFFNIKCRYSGLCPHVVVLVATVRALKMHGGGPTVTAGLPLPKAYIQENLELVEKGFSNLKKQIENARMFGIPVVVAVNAFKTDTESELDLISRLSREHGAFDAVKCTHWAEGGKGALALAQAVQRAAQAPSSFQLLYDLKLPVEDKIRIIAQKIYGADDIELLPEAQHKAEVYTKQGFGNLPICMAKTHLSLSHNPEQKGVPTGFILPIRDIRASVGAGFLYPLVGTMSTMPGLPTRPCFYDIDLDPETEQVNGLF

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Dog
100
Rabbit
100
Bovine
91
Sheep
91
Horse
82
Xenopus
82
Chicken
82
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P11586 作为底物

Site PTM Type Enzyme
M1 Acetylation
K10 Acetylation
K10 Ubiquitination
K21 Ubiquitination
T25 Phosphorylation
K28 Ubiquitination
T35 Phosphorylation
R37 Methylation
Y52 Phosphorylation
K56 Acetylation
K58 Ubiquitination
K66 Ubiquitination
K71 Ubiquitination
T148 Phosphorylation
K150 Ubiquitination
K157 Ubiquitination
C195 S-Nitrosylation
K198 Ubiquitination
K223 Sumoylation
K223 Ubiquitination
K228 Ubiquitination
K251 Ubiquitination
Y258 Phosphorylation
K262 Acetylation
K262 Ubiquitination
S266 Phosphorylation
T269 Phosphorylation
T279 Phosphorylation
K313 Ubiquitination
T314 Phosphorylation
S318 Phosphorylation
R324 Methylation
C326 S-Nitrosylation
K333 Ubiquitination
K352 Ubiquitination
K354 Ubiquitination
S358 Phosphorylation
K364 Ubiquitination
K370 Ubiquitination
Y402 Phosphorylation
S413 Phosphorylation
T471 Phosphorylation
K473 Acetylation
K473 Ubiquitination
R488 Methylation
S490 Phosphorylation
R495 Methylation
K504 Ubiquitination
T505 Phosphorylation
T511 Phosphorylation
R539 Methylation
K543 Ubiquitination
T545 Phosphorylation
K553 Acetylation
K553 Ubiquitination
K588 Ubiquitination
S601 Phosphorylation
S608 Phosphorylation
T612 Phosphorylation
K684 Ubiquitination
K721 Acetylation
K721 Ubiquitination
K733 Ubiquitination
K739 Acetylation
K739 Ubiquitination
T761 Phosphorylation
T763 Phosphorylation
S765 Phosphorylation
S771 Phosphorylation
K784 Ubiquitination
K793 Acetylation
K793 Ubiquitination
K819 Acetylation
K819 Ubiquitination
K825 Ubiquitination
K832 Ubiquitination
Y834 Phosphorylation
K848 Ubiquitination
K854 Ubiquitination
K866 Ubiquitination
S870 Phosphorylation
S872 Phosphorylation
T882 Phosphorylation

研究背景

细胞定位:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitous.

亚基结构:

Homodimer.

蛋白家族:

This trifunctional enzyme consists of two major domains: an N-terminal part containing the methylene-THF dehydrogenase and cyclohydrolase activities and a larger C-terminal part containing formyl-THF synthetase activity.

In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.

In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family.

研究领域

· Metabolism > Metabolism of cofactors and vitamins > One carbon pool by folate.

· Metabolism > Global and overview maps > Metabolic pathways.

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.