Clathrin heavy chain Antibody - #DF13223

(6)  
产品: Clathrin heavy chain 抗体
货号: DF13223
描述: Rabbit polyclonal antibody to Clathrin heavy chain
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Zebrafish, Rabbit, Dog, Chicken
分子量: 187kDa; 187kD(Calculated).
蛋白号: P53675
RRID: AB_2846242

浏览相似产品>>

   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Zebrafish(88%), Rabbit(88%), Dog(100%), Chicken(88%)
克隆:
Polyclonal
特异性:
Clathrin heavy chain Antibody detects endogenous levels of total Clathrin heavy chain.
RRID:
AB_2846242
引用格式: Affinity Biosciences Cat# DF13223, RRID:AB_2846242.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

抗原和靶标

免疫原:
Uniprot:

P53675(CLH2_HUMAN) >>Visit HPA database.

基因/基因ID:
CLTCL1(8218)
表达:
P53675 CLH2_HUMAN:

Maximal levels in skeletal muscle. High levels in heart and testis. Low expression detected in all other tissues.

序列:
MAQILPVRFQEHFQLQNLGINPANIGFSTLTMESDKFICIREKVGEQAQVTIIDMSDPMAPIRRPISAESAIMNPASKVIALKAGKTLQIFNIEMKSKMKAHTMAEEVIFWKWVSVNTVALVTETAVYHWSMEGDSQPMKMFDRHTSLVGCQVIHYRTDEYQKWLLLVGISAQQNRVVGAMQLYSVDRKVSQPIEGHAAAFAEFKMEGNAKPATLFCFAVRNPTGGKLHIIEVGQPAAGNQPFVKKAVDVFFPPEAQNDFPVAMQIGAKHGVIYLITKYGYLHLYDLESGVCICMNRISADTIFVTAPHKPTSGIIGVNKKGQVLSVCVEEDNIVNYATNVLQNPDLGLRLAVRSNLAGAEKLFVRKFNTLFAQGSYAEAAKVAASAPKGILRTRETVQKFQSIPAQSGQASPLLQYFGILLDQGQLNKLESLELCHLVLQQGRKQLLEKWLKEDKLECSEELGDLVKTTDPMLALSVYLRANVPSKVIQCFAETGQFQKIVLYAKKVGYTPDWIFLLRGVMKISPEQGLQFSRMLVQDEEPLANISQIVDIFMENSLIQQCTSFLLDALKNNRPAEGLLQTWLLEMNLVHAPQVADAILGNKMFTHYDRAHIAQLCEKAGLLQQALEHYTDLYDIKRAVVHTHLLNPEWLVNFFGSLSVEDSVECLHAMLSANIRQNLQLCVQVASKYHEQLGTQALVELFESFKSYKGLFYFLGSIVNFSQDPDVHLKYIQAACKTGQIKEVERICRESSCYNPERVKNFLKEAKLTDQLPLIIVCDRFGFVHDLVLYLYRNNLQRYIEIYVQKVNPSRTPAVIGGLLDVDCSEEVIKHLIMAVRGQFSTDELVAEVEKRNRLKLLLPWLESQIQEGCEEPATHNALAKIYIDSNNSPECFLRENAYYDSSVVGRYCEKRDPHLACVAYERGQCDLELIKVCNENSLFKSEARYLVCRKDPELWAHVLEETNPSRRQLIDQVVQTALSETRDPEEISVTVKAFMTADLPNELIELLEKIVLDNSVFSEHRNLQNLLILTAIKADRTRVMEYISRLDNYDALDIASIAVSSALYEEAFTVFHKFDMNASAIQVLIEHIGNLDRAYEFAERCNEPAVWSQLAQAQLQKDLVKEAINSYIRGDDPSSYLEVVQSASRSNNWEDLVKFLQMARKKGRESYIETELIFALAKTSRVSELEDFINGPNNAHIQQVGDRCYEEGMYEAAKLLYSNVSNFARLASTLVHLGEYQAAVDNSRKASSTRTWKEVCFACMDGQEFRFAQLCGLHIVIHADELEELMCYYQDRGYFEELILLLEAALGLERAHMGMFTELAILYSKFKPQKMLEHLELFWSRVNIPKVLRAAEQAHLWAELVFLYDKYEEYDNAVLTMMSHPTEAWKEGQFKDIITKVANVELCYRALQFYLDYKPLLINDLLLVLSPRLDHTWTVSFFSKAGQLPLVKPYLRSVQSHNNKSVNEALNHLLTEEEDYQGLRASIDAYDNFDNISLAQQLEKHQLMEFRCIAAYLYKGNNWWAQSVELCKKDHLYKDAMQHAAESRDAELAQKLLQWFLEEGKRECFAACLFTCYDLLRPDMVLELAWRHNLVDLAMPYFIQVMREYLSKVDKLDALESLRKQEEHVTEPAPLVFDFDGHE

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Dog
100
Zebrafish
88
Chicken
88
Rabbit
88
Pig
0
Horse
0
Bovine
0
Sheep
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P53675 作为底物

Site PTM Type Enzyme
K78 Ubiquitination
K83 Ubiquitination
K86 Ubiquitination
K96 Ubiquitination
T103 Phosphorylation
S171 Phosphorylation
T214 Phosphorylation
T224 Phosphorylation
K227 Ubiquitination
K245 Ubiquitination
K246 Ubiquitination
K362 Ubiquitination
K367 Ubiquitination
S386 Phosphorylation
K389 Ubiquitination
K450 Acetylation
K450 Ubiquitination
K453 Ubiquitination
K456 Acetylation
K456 Ubiquitination
S477 Phosphorylation
K506 Acetylation
K507 Ubiquitination
Y510 Phosphorylation
K619 Ubiquitination
S672 Phosphorylation
K737 Ubiquitination
K742 Acetylation
K742 Ubiquitination
Y754 Phosphorylation
K764 Acetylation
K764 Ubiquitination
Y799 Phosphorylation
Y803 Phosphorylation
K806 Ubiquitination
S841 Phosphorylation
T842 Phosphorylation
K851 Ubiquitination
K856 Ubiquitination
S889 Phosphorylation
K911 Ubiquitination
K932 Ubiquitination
K941 Ubiquitination
K951 Ubiquitination
T963 Phosphorylation
S966 Phosphorylation
S989 Phosphorylation
S1016 Phosphorylation
S1019 Phosphorylation
K1034 Ubiquitination
T1038 Phosphorylation
Y1043 Phosphorylation
S1045 Phosphorylation
Y1096 Phosphorylation
K1122 Ubiquitination
Y1168 Phosphorylation
T1171 Phosphorylation
S1181 Phosphorylation
T1318 Phosphorylation
S1341 Phosphorylation
K1347 Ubiquitination
K1387 Ubiquitination
K1392 Ubiquitination
T1396 Phosphorylation
K1449 Acetylation
K1449 Ubiquitination
K1461 Ubiquitination
S1462 Phosphorylation
K1529 Ubiquitination
K1535 Ubiquitination
K1612 Ubiquitination
K1621 Ubiquitination

研究背景

功能:

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network (By similarity).

细胞定位:

Cytoplasmic vesicle membrane>Peripheral membrane protein>Cytoplasmic side. Membrane>Coated pit>Peripheral membrane protein>Cytoplasmic side.
Note: Cytoplasmic face of coated pits and vesicles.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Maximal levels in skeletal muscle. High levels in heart and testis. Low expression detected in all other tissues.

亚基结构:

Clathrin triskelions, composed of 3 heavy chains and 3 light chains, are the basic subunits of the clathrin coat. In the presence of light chains, hub assembly is influenced by both the pH and the concentration of calcium (By similarity). May interact with OCRL (By similarity).

蛋白家族:

The C-terminal third of the heavy chains forms the hub of the triskelion. This region contains the trimerization domain and the light-chain binding domain involved in the assembly of the clathrin lattice.

The N-terminal seven-bladed beta-propeller is formed by WD40-like repeats, and projects inward from the polyhedral outer clathrin coat. It constitutes a major protein-protein interaction node (By similarity).

Belongs to the clathrin heavy chain family.

研究领域

· Cellular Processes > Transport and catabolism > Lysosome.   (View pathway)

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

· Organismal Systems > Nervous system > Synaptic vesicle cycle.

· Organismal Systems > Excretory system > Endocrine and other factor-regulated calcium reabsorption.

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.