Phospho-p70 S6 Kinase beta (Ser423) Antibody - #AF3486

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产品: 磷酸化 p70 S6 Kinase beta (Ser423) 抗体
货号: AF3486
描述: Rabbit polyclonal antibody to Phospho-p70 S6 Kinase beta (Ser423)
应用: WB IF/ICC
反应: Human, Mouse
预测: Pig, Bovine, Horse, Sheep, Dog
分子量: 50~70kD; 53kD(Calculated).
蛋白号: Q9UBS0
RRID: AB_2834924

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(91%), Bovine(91%), Horse(82%), Sheep(91%), Dog(91%)
克隆:
Polyclonal
特异性:
Phospho-p70 S6 Kinase beta (Ser423) Antibody detects endogenous levels of p70 S6 Kinase beta only when phosphorylated at Serine 423.
RRID:
AB_2834924
引用格式: Affinity Biosciences Cat# AF3486, RRID:AB_2834924.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

70 kDa ribosomal protein S6 kinase 2; EC 2.7.11.1; KS6B2_HUMAN; p70 beta; p70 ribosomal S6 kinase beta; p70 S6 kinase beta; p70 S6K-beta; p70 S6KB; p70 S6Kbeta; p70(S6K) beta; p70-beta; p70-S6K 2; P70S6K2; p70S6Kb; Ribosomal protein S6 kinase 70kDa, polypeptide 2; Ribosomal protein S6 kinase beta 2; Ribosomal protein S6 kinase beta-2; Rps6kb2; S6 kinase related kinase; S6 kinase-related kinase; S6K beta 2; S6K beta; S6K-beta; S6K-beta-2; S6K2; Serine/threonine protein kinase 14 beta; Serine/threonine-protein kinase 14B; SRK; STK14B;

抗原和靶标

免疫原:
Uniprot:

Q9UBS0(KS6B2_HUMAN) >>Visit HPA database.

基因/基因ID:
RPS6KB2(6199)
描述:
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates the S6 ribosomal protein and eucaryotic translation initiation factor 4B (eIF4B).
序列:
MAAVFDLDLETEEGSEGEGEPELSPADACPLAELRAAGLEPVGHYEEVELTETSVNVGPERIGPHCFELLRVLGKGGYGKVFQVRKVQGTNLGKIYAMKVLRKAKIVRNAKDTAHTRAERNILESVKHPFIVELAYAFQTGGKLYLILECLSGGELFTHLEREGIFLEDTACFYLAEITLALGHLHSQGIIYRDLKPENIMLSSQGHIKLTDFGLCKESIHEGAVTHTFCGTIEYMAPEILVRSGHNRAVDWWSLGALMYDMLTGSPPFTAENRKKTMDKIIRGKLALPPYLTPDARDLVKKFLKRNPSQRIGGGPGDAADVQRHPFFRHMNWDDLLAWRVDPPFRPCLQSEEDVSQFDTRFTRQTPVDSPDDTALSESANQAFLGFTYVAPSVLDSIKEGFSFQPKLRSPRRLNSSPRAPVSPLKFSPFEGFRPSPSLPEPTELPLPPLLPPPPPSTTAPLPIRPPSGTKKSKRGRGRPGR

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
91
Bovine
91
Sheep
91
Dog
91
Horse
82
Chicken
73
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9UBS0 作为底物

Site PTM Type Enzyme
S15 Phosphorylation
S24 Phosphorylation
Y45 Phosphorylation P12931 (SRC)
K75 Ubiquitination
K80 Ubiquitination
K86 Ubiquitination
K94 Ubiquitination
Y96 Phosphorylation
K111 Ubiquitination
Y136 Phosphorylation
K196 Ubiquitination
S219 Phosphorylation
T226 Phosphorylation
T228 Phosphorylation O15530 (PDPK1)
S254 Phosphorylation
S266 Phosphorylation
T270 Phosphorylation
K276 Acetylation
K280 Ubiquitination
K285 Ubiquitination
S356 Phosphorylation
T388 Phosphorylation P42345 (MTOR)
K407 Ubiquitination
S410 Phosphorylation
S416 Phosphorylation
S417 Phosphorylation
S423 Phosphorylation
S473 Phosphorylation P05771-2 (PRKCB) , P05129 (PRKCG) , Q05513 (PRKCZ) , P41743 (PRKCI) , Q05655 (PRKCD) , Q02156 (PRKCE) , P17252 (PRKCA)

翻译修饰 - Q9UBS0 作为激酶

Substrate Site Source
O00418 (EEF2K) S366 Uniprot
P04792 (HSPB1) S78 Uniprot
P04792 (HSPB1) S82 Uniprot
P05783 (KRT18) S53 Uniprot
P09651 (HNRNPA1) S4 Uniprot
P09651 (HNRNPA1) S6 Uniprot
P49841 (GSK3B) S9 Uniprot
Q05195 (MXD1) S145 Uniprot
Q53EL6 (PDCD4) S67 Uniprot
Q6R327 (RICTOR) T1135 Uniprot

研究背景

功能:

Phosphorylates specifically ribosomal protein S6. Seems to act downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression in an alternative pathway regulated by MEAK7.

翻译修饰:

Phosphorylated and activated by MTOR. Phosphorylation by PKC within the NLS in response to mitogenic stimuli causes cytoplasmic retention.

细胞定位:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
蛋白家族:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.

研究领域

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Choline metabolism in cancer.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway - multiple species.   (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

文献引用

1). Notch1/Hes1‑PTEN/AKT/IL‑17A feedback loop regulates Th17 cell differentiation in mouse psoriasis‑like skin inflammation. Molecular Medicine Reports (PubMed: 35582997) [IF=3.4]

Application: WB    Species: mouse    Sample: skin

Figure 6. - Protein levels of (A) Notch1, (B) NICD1, (C) hairy and enhancer of split 1, (D) PTEN, (E-1) p-AKT (Thr308), (E-2) AKT, (E-3) p-AKT (Ser473), (F-1) p-mTOR (Ser2448), (F-2) mTORC1, (G) S6K1 (p70S6Kα), (H) S6K2 (p70S6Kβ) and (I) IL-17A in skin samples of experimental mice. *P

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