MafA Antibody - #DF13429

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产品: MafA 抗体
货号: DF13429
描述: Rabbit polyclonal antibody to MafA
应用: WB
反应: Human, Mouse, Rat
预测: Bovine, Dog, Chicken
分子量: 36kDa; 37kD(Calculated).
蛋白号: Q8NHW3
RRID: AB_2846448

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Bovine(88%), Dog(88%), Chicken(100%)
克隆:
Polyclonal
特异性:
MafA Antibody detects endogenous levels of total MafA.
RRID:
AB_2846448
引用格式: Affinity Biosciences Cat# DF13429, RRID:AB_2846448.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

HMafA; Mafa; MAFA_HUMAN; Pancreatic beta cell specific transcriptional activator; Pancreatic beta-cell-specific transcriptional activator; RIPE3b1; Transcription factor MafA; Transcription factor RIPE3b1; v maf musculoaponeurotic fibrosarcoma oncogene homolog A (avian); v maf musculoaponeurotic fibrosarcoma oncogene homolog A; V-maf musculoaponeurotic fibrosarcoma oncogene homolog A;

抗原和靶标

免疫原:
Uniprot:

Q8NHW3(MAFA_HUMAN) >>Visit HPA database.

基因/基因ID:
MAFA(389692)
表达:
Q8NHW3 MAFA_HUMAN:

Expressed in the islets of Langerhans (at protein level).

序列:
MAAELAMGAELPSSPLAIEYVNDFDLMKFEVKKEPPEAERFCHRLPPGSLSSTPLSTPCSSVPSSPSFCAPSPGTGGGGGAGGGGGSSQAGGAPGPPSGGPGAVGGTSGKPALEDLYWMSGYQHHLNPEALNLTPEDAVEALIGSGHHGAHHGAHHPAAAAAYEAFRGPGFAGGGGADDMGAGHHHGAHHAAHHHHAAHHHHHHHHHHGGAGHGGGAGHHVRLEERFSDDQLVSMSVRELNRQLRGFSKEEVIRLKQKRRTLKNRGYAQSCRFKRVQQRHILESEKCQLQSQVEQLKLEVGRLAKERDLYKEKYEKLAGRGGPGSAGGAGFPREPSPPQAGPGGAKGTADFFL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Chicken
100
Bovine
88
Dog
88
Pig
0
Horse
0
Sheep
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q8NHW3 作为底物

Site PTM Type Enzyme
S14 Phosphorylation P28482 (MAPK1) , Q13233 (MAP3K1) , Q16539 (MAPK14)
K32 Sumoylation
S49 Phosphorylation P49840 (GSK3A) , P49841 (GSK3B)
T53 Phosphorylation P49841 (GSK3B) , P49840 (GSK3A)
T57 Phosphorylation P49840 (GSK3A) , Q16539 (MAPK14) , P49841 (GSK3B) , O15264 (MAPK13)
S61 Phosphorylation P49841 (GSK3B) , P49840 (GSK3A)
S65 Phosphorylation P28482 (MAPK1) , Q13233 (MAP3K1)
T134 Phosphorylation O15264 (MAPK13) , Q16539 (MAPK14)
Y314 Phosphorylation
S336 Phosphorylation O15264 (MAPK13)

研究背景

功能:

Transcription factor that activates insulin gene expression. Acts synergistically with NEUROD1/BETA2 and PDX1. Binds the insulin enhancer C1/RIPE3b element. Binds to consensus TRE-type MARE 5'-TGCTGACTCAGCA-3' DNA sequence.

翻译修饰:

Ubiquitinated, leading to its degradation by the proteasome.

Phosphorylated at tyrosines.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in the islets of Langerhans (at protein level).

亚基结构:

Forms homodimers or heterodimers. Monomers and dimers are able to bind DNA, but the off-rate is faster for monomers. Interacts with NEUROD1 and PDX1 (By similarity). May interact with MAFB, FOS, JUN and PCAF (By similarity).

蛋白家族:

Belongs to the bZIP family. Maf subfamily.

研究领域

· Human Diseases > Endocrine and metabolic diseases > Type II diabetes mellitus.

· Human Diseases > Endocrine and metabolic diseases > Maturity onset diabetes of the young.

文献引用

1). Inhibition of miR-146a-5p and miR-8114 in Insulin-Secreting Cells Contributes to the Protection of Melatonin against Stearic Acid-Induced Cellular Senescence by Targeting Mafa. Endocrinology and Metabolism (PubMed: 36475359) [IF=3.4]

Application: WB    Species: Mouse    Sample: β-TC6 cells

Fig. 6. V-maf musculoaponeurotic fibrosarcoma oncogene homolog A (Mafa) is a direct target of miR-146a-5p and miR-8114. (A) Effect of melatonin on Mafa protein levels in stearic acid (SA)-treated β-TC6 cells. (B, C) Predicted binding sites between miR-146a/miR-8114 and Mafa, respectively. (D, E) Changes in levels of Mafa mRNA and Mafa protein after transfection of anti-miRNA oligonucleotides (AMO)- 146a-5p in SA-treated β-TC6 cells, respectively. (F, G) Mafa mRNA and Mafa protein levels after inhibition of miR-8114 in the presence of SA, respectively. (H, I) Overexpression of miR-146a-5p decreased Mafa mRNA and Mafa protein levels in the absence of SA, respectively. (J, K) Transfection of miR-8114 mimic alone inhibited the expression of Mafa mRNA and Mafa protein, respectively. (L) Luciferase activity between Mafa and miR-146a-5p or miR-8114, respectively. Each experiment was repeated independently three (A-K) or four (L) times. UTR, untranslated region; WT, wild-type; MUT, mutant; NS, no significance. aP

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