Phospho-MLC2 (Ser19) Antibody - #AF3829
| 产品: | 磷酸化 MLC2 (Ser19) 抗体 |
| 货号: | AF3829 |
| 描述: | Rabbit polyclonal antibody to Phospho-MLC2 (Ser19) |
| 应用: | IHC IF/ICC |
| 反应: | Human, Mouse, Rat |
| 分子量: | 19kD(Calculated). |
| 蛋白号: | P10916 |
| RRID: | AB_2847143 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3829, RRID:AB_2847143.
展开/折叠
Cardiac myosin light chain-2; Cardiac ventricular myosin light chain 2; CMH10; MLC 2v; MLC-2; MLC-2v; MLC2; MLRV_HUMAN; MYL 2; MYL2; Myosin light chain 2 regulatory cardiac slow; Myosin light polypeptide 2 regulatory cardiac slow; Myosin regulatory light chain 2; Myosin regulatory light chain 2 ventricular/cardiac muscle isoform; Regulatory light chain of myosin; RLC of myosin; Slow cardiac myosin regulatory light chain 2; ventricular/cardiac muscle isoform;
抗原和靶标
A synthesized peptide derived from human MLC2 around the phosphorylation site of Ser19.
- P10916 MLRV_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAPKKAKKRAGGANSNVFSMFEQTQIQEFKEAFTIMDQNRDGFIDKNDLRDTFAALGRVNVKNEEIDEMIKEAPGPINFTVFLTMFGEKLKGADPEETILNAFKVFDPEGKGVLKADYVREMLTTQAERFSKEEVDQMFAAFPPDVTGNLDYKNLVHIITHGEEKD
翻译修饰 - P10916 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S15 | Phosphorylation | Q15746 (MYLK) | Uniprot |
| S19 | Phosphorylation | Q5VT25 (CDC42BPA) | Uniprot |
| T80 | Phosphorylation | Uniprot | |
| T98 | Phosphorylation | Uniprot | |
| K104 | Acetylation | Uniprot | |
| K111 | Acetylation | Uniprot | |
| Y118 | Phosphorylation | Uniprot | |
| T124 | Phosphorylation | Uniprot | |
| Y152 | Phosphorylation | Uniprot | |
| K153 | Acetylation | Uniprot | |
| K165 | Acetylation | Uniprot |
研究背景
Contractile protein that plays a role in heart development and function (By similarity). Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force (By similarity). During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly (By similarity).
N-terminus is methylated by METTL11A/NTM1.
Phosphorylated by MYLK3 and MYLK2; promotes cardiac muscle contraction and function (By similarity). Dephosphorylated by PPP1CB complexed to PPP1R12B (By similarity). The phosphorylated form in adult is expressed as gradients across the heart from endocardium (low phosphorylation) to epicardium (high phosphorylation); regulates cardiac torsion and workload distribution (By similarity).
Cytoplasm>Myofibril>Sarcomere>A band.
Myosin is a hexamer of 2 heavy chains and 4 light chains. Interacts with MYOC.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Environmental Information Processing > Signal transduction > Apelin signaling pathway. (View pathway)
· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).
· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).
· Organismal Systems > Circulatory system > Cardiac muscle contraction. (View pathway)
· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes. (View pathway)
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
限制条款
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