产品: 磷酸化 IRF7 (Ser471/Ser472) 抗体
货号: AF3885
描述: Rabbit polyclonal antibody to Phospho-IRF7 (Ser471/Ser472)
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
分子量: 50~70kD; 54kD(Calculated).
蛋白号: Q92985
RRID: AB_2847199

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 50ul RMB¥ 1500 现货
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

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产品描述

来源:
Rabbit
应用:
IHC 1:50-1:200, IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
克隆:
Polyclonal
特异性:
Phospho-IRF7 (Ser471/Ser472) Antibody detects endogenous levels of IRF7 only when phosphorylated at Ser471/472.
RRID:
AB_2847199
引用格式: Affinity Biosciences Cat# AF3885, RRID:AB_2847199.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

IMD39; Interferon regulatory factor 7; Interferon regulatory factor 7H; IRF 7; IRF 7A; IRF 7H; IRF-7; IRF7; IRF7_HUMAN; IRF7A; IRF7B; IRF7C; IRF7H;

抗原和靶标

免疫原:

A synthesized peptide derived from human IRF7 around the phosphorylation site of Ser471/472.

Uniprot:
基因/基因ID:
表达:
Q92985 IRF7_HUMAN:

Expressed predominantly in spleen, thymus and peripheral blood leukocytes.

序列:
MALAPERAAPRVLFGEWLLGEISSGCYEGLQWLDEARTCFRVPWKHFARKDLSEADARIFKAWAVARGRWPPSSRGGGPPPEAETAERAGWKTNFRCALRSTRRFVMLRDNSGDPADPHKVYALSRELCWREGPGTDQTEAEAPAAVPPPQGGPPGPFLAHTHAGLQAPGPLPAPAGDKGDLLLQAVQQSCLADHLLTASWGADPVPTKAPGEGQEGLPLTGACAGGPGLPAGELYGWAVETTPSPGPQPAALTTGEAAAPESPHQAEPYLSPSPSACTAVQEPSPGALDVTIMYKGRTVLQKVVGHPSCTFLYGPPDPAVRATDPQQVAFPSPAELPDQKQLRYTEELLRHVAPGLHLELRGPQLWARRMGKCKVYWEVGGPPGSASPSTPACLLPRNCDTPIFDFRVFFQELVEFRARQRRGSPRYTIYLGFGQDLSAGRPKEKSLVLVKLEPWLCRVHLEGTQREGVSSLDSSSLSLCLSSANSLYDDIECFLMELEQPA

翻译修饰 - Q92985 作为底物

Site PTM Type Enzyme
Ubiquitination
K50 Ubiquitination
S53 Phosphorylation
K61 Ubiquitination
K92 Acetylation
K92 Ubiquitination
K120 Ubiquitination
K303 Ubiquitination
K341 Ubiquitination
K375 Ubiquitination
T391 Phosphorylation
K444 Ubiquitination
K446 Ubiquitination
K452 Ubiquitination
S471 Phosphorylation Q9UHD2 (TBK1) , Q14164 (IKBKE)
S472 Phosphorylation Q9UHD2 (TBK1) , Q14164 (IKBKE)
S477 Phosphorylation Q14164 (IKBKE) , Q9UHD2 (TBK1)
S479 Phosphorylation Q14164 (IKBKE) , Q9UHD2 (TBK1)
S483 Phosphorylation
S487 Phosphorylation

研究背景

功能:

Key transcriptional regulator of type I interferon (IFN)-dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction via both the virus-activated, MyD88-independent pathway and the TLR-activated, MyD88-dependent pathway. Induces transcription of ubiquitin hydrolase USP25 mRNA in response to lipopolysaccharide (LPS) or viral infection in a type I IFN-dependent manner (By similarity). Required during both the early and late phases of the IFN gene induction but is more critical for the late than for the early phase. Exists in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization where along with other coactivators it can activate transcription of the type I IFN and ISG genes. Can also play a role in regulating adaptive immune responses by inducing PSMB9/LMP2 expression, either directly or through induction of IRF1. Binds to the Q promoter (Qp) of EBV nuclear antigen 1 a (EBNA1) and may play a role in the regulation of EBV latency. Can activate distinct gene expression programs in macrophages and regulate the anti-tumor properties of primary macrophages.

翻译修饰:

Acetylation inhibits its DNA-binding ability and activity.

In response to a viral infection, phosphorylated on Ser-477 and Ser-479 by TBK1 and IKBKE1. Phosphorylation, and subsequent activation is inhibited by vaccinia virus protein E3. In TLR7- and TLR9-mediated signaling pathway, phosphorylated by IRAK1.

TRAF6-mediated ubiquitination is required for IRF7 activation.

Sumoylated by TRIM28, which inhibits its transactivation activity.

(Microbial infection) Cleaved and inactivated by the protease 3C of enterovirus 71 allowing the virus to disrupt the host type I interferon production.

(Microbial infection) Cleaved and inactivated by the protease 3C of human enterovirus 68D (EV68) allowing the virus to disrupt the host type I interferon production.

细胞定位:

Nucleus. Cytoplasm.
Note: The phosphorylated and active form accumulates selectively in the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed predominantly in spleen, thymus and peripheral blood leukocytes.

亚基结构:

Monomer. Homodimer; phosphorylation-induced. Heterodimer with IRF3. Interacts with TICAM1 and TICAM2. Interacts with MYD88 AND TRAF6.

(Microbial infection) Interacts with Epstein-Barr virus LF2 and LMP1.

(Microbial infection) Interacts with rotavirus A NSP1; this interaction leads to the proteasome-dependent degradation of IRF7.

(Microbial infection) Interacts with human herpes virus 8/HHV-8 proteins ORF45 and vIRF-1.

(Microbial infection) Interacts with human T-cell leukemia virus 1/HTLV-1 protein HBZ.

(Microbial infection) Interacts with Seneca Valley virus protease 3C; this interaction is involved in the suppression of IRF7 expression and phosphorylation by the virus.

蛋白家族:

Belongs to the IRF family.

研究领域

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway.   (View pathway)

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