产品: XPA 抗体
货号: AF0294
描述: Rabbit polyclonal antibody to XPA
应用: WB IHC
反应: Human, Mouse
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Xenopus
分子量: 40kDa; 31kD(Calculated).
蛋白号: P23025
RRID: AB_2833460

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 50ul RMB¥ 1250 现货
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 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(88%), Zebrafish(88%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Xenopus(88%)
克隆:
Polyclonal
特异性:
XPA Antibody detects endogenous levels of total XPA.
RRID:
AB_2833460
引用格式: Affinity Biosciences Cat# AF0294, RRID:AB_2833460.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

DNA repair protein complementing XP A cells; DNA repair protein complementing XP-A cells; DNA repair protein complementing XPA cells; Excision repair controlling; Xeroderma pigmentosum 1; Xeroderma pigmentosum complementation group A; Xeroderma pigmentosum group A complementing protein; Xeroderma pigmentosum group A-complementing protein; XP 1; XP1; xpa; XPA_HUMAN; Xpac;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
P23025 XPA_HUMAN:

Expressed in various cell lines and in skin fibroblasts.

描述:
XPA Involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation.
序列:
MAAADGALPEAAALEQPAELPASVRASIERKRQRALMLRQARLAARPYSATAAAATGGMANVKAAPKIIDTGGGFILEEEEEEEQKIGKVVHQPGPVMEFDYVICEECGKEFMDSYLMNHFDLPTCDNCRDADDKHKLITKTEAKQEYLLKDCDLEKREPPLKFIVKKNPHHSQWGDMKLYLKLQIVKRSLEVWGSQEALEEAKEVRQENREKMKQKKFDKKVKELRRAVRSSVWKRETIVHQHEYGPEENLEDDMYRKTCTMCGHELTYEKM

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Pig
88
Xenopus
88
Zebrafish
88
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P23025 作为底物

Site PTM Type Enzyme
A2 Acetylation
Y48 Phosphorylation
K63 Sumoylation
K67 Ubiquitination
K86 Sumoylation
K141 Sumoylation
K141 Ubiquitination
K145 Sumoylation
K145 Ubiquitination
K151 Ubiquitination
K157 Acetylation
K168 Ubiquitination
S173 Phosphorylation P78527 (PRKDC) , Q13535 (ATR) , Q13315 (ATM)
S196 Phosphorylation P78527 (PRKDC) , Q13315 (ATM) , Q13535 (ATR)
K204 Ubiquitination
T239 Phosphorylation
Y257 Phosphorylation
K259 Ubiquitination

研究背景

功能:

Involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHEK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation.

翻译修饰:

ATR-dependent phosphorylation of XPA at Ser-196 is important for cell survival in response to UV damage.

Ubiquitinated by HERC2 leading to degradation by the proteasome.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in various cell lines and in skin fibroblasts.

亚基结构:

Interacts with GPN1. Interacts with RPA1 and RPA2; the interaction is direct and associates XPA with the RPA complex. Interacts (via N-terminus) with CEP164 upon UV irradiation. Interacts with HERC2.

蛋白家族:

Belongs to the XPA family.

研究领域

· Genetic Information Processing > Replication and repair > Nucleotide excision repair.

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

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