Myelin Basic Protein/MBP Antibody - #AF4085

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产品: Myelin Basic Protein/MBP 抗体
货号: AF4085
描述: Rabbit polyclonal antibody to Myelin Basic Protein/MBP
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
分子量: 14~33kd; 33kD(Calculated).
蛋白号: P02686
RRID: AB_2835364

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(86%), Xenopus(86%)
克隆:
Polyclonal
特异性:
Myelin Basic Protein/MBP Antibody detects endogenous levels of total Myelin Basic Protein/MBP.
RRID:
AB_2835364
引用格式: Affinity Biosciences Cat# AF4085, RRID:AB_2835364.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

GDB; Golli MBP; Golli MBP; myelin basic protein; Hemopoietic MBP; HMBPR; HUGO; MBP; MBP_CAVPO; MBP_HUMAN; MGC99675; MLD; Myelin A1 protein; Myelin A1 Protein, basic; Myelin basic protein; Myelin Deficient; Myelin membrane encephalitogenic protein; OTTHUMP00000163776; OTTHUMP00000174387; OTTHUMP00000174388; SHI; Shiverer; SP;

抗原和靶标

免疫原:
Uniprot:

P02686(MBP_HUMAN) >>Visit HPA database.

基因/基因ID:
MBP(4155)
表达:
P02686 MBP_HUMAN:

MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.

描述:
The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
序列:
MGNHAGKRELNAEKASTNSETNRGESEKKRNLGELSRTTSEDNEVFGEADANQNNGTSSQDTAVTDSKRTADPKNAWQDAHPADPGSRPHLIRLFSRDAPGREDNTFKDRPSESDELQTIQEDSAATSESLDVMASQKRPSQRHGSKYLATASTMDHARHGFLPRHRDTGILDSIGRFFGGDRGAPKRGSGKDSHHPARTAHYGSLPQKSHGRTQDENPVVHFFKNIVTPRTPPPSQGKGRGLSLSRFSWGAEGQRPGFGYGGRASDYKSAHKGFKGVDAQGTLSKIFKLGGRDSRSGSPMARR

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
100
Xenopus
86
Chicken
86
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P02686 作为底物

Site PTM Type Enzyme
S16 Phosphorylation
S19 Phosphorylation
S26 Phosphorylation
S36 Phosphorylation
T38 Phosphorylation
T39 Phosphorylation
S40 Phosphorylation
T65 Phosphorylation
S87 Phosphorylation
S96 Phosphorylation P27361 (MAPK3)
T106 Phosphorylation
S114 Phosphorylation
S141 Phosphorylation
S146 Phosphorylation P17612 (PRKACA)
Y148 Phosphorylation
S153 Phosphorylation
T154 Phosphorylation
T169 Phosphorylation P17612 (PRKACA)
S190 Phosphorylation P17612 (PRKACA)
Y203 Phosphorylation
S205 Phosphorylation
T229 Phosphorylation P28482 (MAPK1)
T232 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3)
R241 Methylation
S249 Phosphorylation P17612 (PRKACA)
Y261 Phosphorylation
S266 Phosphorylation P17612 (PRKACA)
T283 Phosphorylation
S285 Phosphorylation
S295 Phosphorylation P17612 (PRKACA)
S299 Phosphorylation Q8TAS1 (UHMK1)

研究背景

功能:

The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.

翻译修饰:

Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.

The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).

Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.

Phosphorylated by TAOK2, VRK2, MAPK11, MAPK12, MAPK14 and MINK1.

细胞定位:

Plasma membrane>Myelin membrane>Peripheral membrane protein>Cytoplasmic side.
Note: Cytoplasmic side of myelin.

Nucleus.
Note: Targeted to nucleus in oligodendrocytes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.

亚基结构:

Homodimer. Isoform 3 exists as a homodimer.

蛋白家族:

Belongs to the myelin basic protein family.

文献引用

1). The spatial arrangement of cells in a 3D-printed biomimetic spinal cord promotes directional differentiation and repairs the motor function after spinal cord injury. Biofabrication, 2021 [IF=9.0]
2). EAAT3 impedes oligodendrocyte remyelination in chronic cerebral hypoperfusion-induced white matter injury. CNS neuroscience & therapeutics, 2024 (PubMed: 37803915) [IF=5.5]

Application: IF/ICC    Species: Mouse    Sample:

FIGURE 4 PDC administration increases the differentiation of OPCs. (A) Representative immunofluorescence co‐staining images of MBP (red) and Olig2 (green) in primary oligodendrocytes. (B) Quantitative analysis of the number of MBP+/ Olig2+ cells in each field of the indicated groups. n = 3–5 images from at least three independent experiments. (C) The process extensions for primary oligodendrocytes were analyzed using Sholl analysis. By counting the intersections that processes make with concentric circles that are numbered 1–3 to represent increasing distance from the cell body, branching was measured, n = 3–5 images from at least three independent experiments. (D, E) Representative immunoblotting and quantification of MBP in each group, n = 3 mice. (F–I) Representative immunofluorescence images and quantification of MBP immunofluorescent intensity in media and paramedian of CC in each field of the indicated groups, n = 3, 3–5 images per animal. There was no difference in body weight between mice in each group. The data for each group conformed to a normal distribution. p Value was determined by ANOVA with Bonferroni's post‐hoc test. *p 

Application: WB    Species: Mouse    Sample:

FIGURE 4 PDC administration increases the differentiation of OPCs. (A) Representative immunofluorescence co‐staining images of MBP (red) and Olig2 (green) in primary oligodendrocytes. (B) Quantitative analysis of the number of MBP+/ Olig2+ cells in each field of the indicated groups. n = 3–5 images from at least three independent experiments. (C) The process extensions for primary oligodendrocytes were analyzed using Sholl analysis. By counting the intersections that processes make with concentric circles that are numbered 1–3 to represent increasing distance from the cell body, branching was measured, n = 3–5 images from at least three independent experiments. (D, E) Representative immunoblotting and quantification of MBP in each group, n = 3 mice. (F–I) Representative immunofluorescence images and quantification of MBP immunofluorescent intensity in media and paramedian of CC in each field of the indicated groups, n = 3, 3–5 images per animal. There was no difference in body weight between mice in each group. The data for each group conformed to a normal distribution. p Value was determined by ANOVA with Bonferroni's post‐hoc test. *p 
3). A new therapeutic strategy targeting protein deacetylation for spinal cord injury. Neuroscience, 2020 (PubMed: 33039524) [IF=3.3]

Application: WB    Species: rat    Sample: spinal cord

Fig.1 |Histopathological detection and behavior tests in injured and sham rats. (D) The acetylation levels of VIM, ALB,and MBP. The total proteins were immunoprecipitated by the corresponding antibody and then subjected to immunoblotting with anti-acetylated-Lys antibody (N=3).
4). Pterostilbene protects the optic nerves and retina in a murine model of experimental autoimmune encephalomyelitis via activation of SIRT1 signaling. NEUROSCIENCE, 2022 (PubMed: 35090883) [IF=3.3]
5). Combination Therapy With Hyperbaric Oxygen and Erythropoietin Inhibits Neuronal Apoptosis and Improves Recovery in Rats With Spinal Cord Injury. Physical Therapy, 2019 (PubMed: 31504911) [IF=3.2]

Application: IHC    Species: rat    Sample: spinal cord tissue

Figure 4 |Hyperbaric oxygen (HBO) and erythropoietin (EPO) downregulate the expression of G protein–coupled receptor 17 (GPR17) and upregulate the expression of myelin basic protein (MBP), indicating improvement in the degree of demyelination.(D) Immunohistochemistry for MBP in the sham, SCI, HBO, EPO, and HBO+EPO groups at 21 days.
6). Analysis of the risk of traumatic brain injury and evaluation neurogranin and myelin basic protein as potential biomarkers of traumatic brain injury in postmortem examination. Forensic Science, Medicine and Pathology, 2022 (PubMed: 35201602) [IF=1.8]

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