TF2H1 Antibody - #DF3155
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3155, RRID:AB_2835532.
展开/折叠
Basic transcription factor 2 62 kDa subunit; Basic transcription factor 62 kDa subunit; BTF 2; BTF2; BTF2 p62; General transcription factor IIH polypeptide 1; General transcription factor IIH subunit 1; General transcription factor IIH, polypeptide 1, 62kDa; gtf2h1; TF2H1; TF2H1_HUMAN; TFB1; TFIIH; TFIIH basal transcription factor complex p62 subunit;
抗原和靶标
- P32780 TF2H1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MATSSEEVLLIVKKVRQKKQDGALYLMAERIAWAPEGKDRFTISHMYADIKCQKISPEGKAKIQLQLVLHAGDTTNFHFSNESTAVKERDAVKDLLQQLLPKFKRKANKELEEKNRMLQEDPVLFQLYKDLVVSQVISAEEFWANRLNVNATDSSSTSNHKQDVGISAAFLADVRPQTDGCNGLRYNLTSDIIESIFRTYPAVKMKYAENVPHNMTEKEFWTRFFQSHYFHRDRLNTGSKDLFAECAKIDEKGLKTMVSLGVKNPLLDLTALEDKPLDEGYGISSVPSASNSKSIKENSNAAIIKRFNHHSAMVLAAGLRKQEAQNEQTSEPSNMDGNSGDADCFQPAVKRAKLQESIEYEDLGKNNSVKTIALNLKKSDRYYHGPTPIQSLQYATSQDIINSFQSIRQEMEAYTPKLTQVLSSSAASSTITALSPGGALMQGGTQQAINQMVPNDIQSELKHLYVAVGELLRHFWSCFPVNTPFLEEKVVKMKSNLERFQVTKLCPFQEKIRRQYLSTNLVSHIEEMLQTAYNKLHTWQSRRLMKKT
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P32780 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y25 | Phosphorylation | Uniprot | |
| K38 | Ubiquitination | Uniprot | |
| T74 | Phosphorylation | Uniprot | |
| K93 | Ubiquitination | Uniprot | |
| K102 | Ubiquitination | Uniprot | |
| S158 | Phosphorylation | Uniprot | |
| Y207 | Phosphorylation | Uniprot | |
| K218 | Ubiquitination | Uniprot | |
| S239 | Phosphorylation | Uniprot | |
| K240 | Acetylation | Uniprot | |
| K240 | Ubiquitination | Uniprot | |
| K275 | Ubiquitination | Uniprot | |
| K293 | Ubiquitination | Uniprot | |
| K296 | Ubiquitination | Uniprot | |
| S339 | Phosphorylation | Uniprot | |
| K353 | Ubiquitination | Uniprot | |
| S357 | Phosphorylation | Uniprot | |
| Y360 | Phosphorylation | Uniprot | |
| K365 | Ubiquitination | Uniprot | |
| S368 | Phosphorylation | Uniprot | |
| K377 | Ubiquitination | Uniprot | |
| T415 | Phosphorylation | Uniprot | |
| K504 | Ubiquitination | Uniprot |
研究背景
Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.
Nucleus.
Component of the 7-subunit TFIIH core complex composed of XPB/ERCC3, XPD/ERCC2, GTF2H1, GTF2H2, GTF2H3, GTF2H4 and GTF2H5, which is active in NER. The core complex associates with the 3-subunit CDK-activating kinase (CAK) module composed of CCNH/cyclin H, CDK7 and MNAT1 to form the 10-subunit holoenzyme (holo-TFIIH) active in transcription. Interacts with PUF60.
Belongs to the TFB1 family.
研究领域
· Genetic Information Processing > Transcription > Basal transcription factors.
· Genetic Information Processing > Replication and repair > Nucleotide excision repair.
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
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