Actinin alpha 2/3 Antibody - #DF3163

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产品: Actinin alpha 2/3 抗体
货号: DF3163
描述: Rabbit polyclonal antibody to Actinin alpha 2/3
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 103 KD; 104kD,103kD(Calculated).
蛋白号: P35609 | Q08043
RRID: AB_2835540

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MS Report
HPLC Report
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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(88%)
克隆:
Polyclonal
特异性:
Actinin alpha 2/3 Antibody detects endogenous levels of total Actinin alpha 2/3.
RRID:
AB_2835540
引用格式: Affinity Biosciences Cat# DF3163, RRID:AB_2835540.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Actin binding protein; Actinin alpha 2; ACTN 2; ACTN2; ACTN2_HUMAN; Alpha actinin 2; Alpha actinin skeletal muscle; Alpha actinin skeletal muscle isoform 2; Alpha-actinin skeletal muscle isoform 2; Alpha-actinin-2; CMD1AA; F actin cross linking protein; F-actin cross-linking protein; Actinin alpha 3; actinin, alpha; ACTN 3; ACTN3; ACTN3_HUMAN; Alpha actinin skeletal muscle; Alpha actinin skeletal muscle isoform 3; Alpha-Actinin 3; Alpha-actinin skeletal muscle isoform 3; Alpha-actinin-3; F actin cross linking protein; F-actin cross-linking protein; MGC117002; MGC117005; OTTHUMP00000219049; OTTHUMP00000219054;

抗原和靶标

免疫原:
Uniprot:

P35609(ACTN2_HUMAN) >>Visit HPA database.

Q08043(ACTN3_HUMAN) >>Visit HPA database.

基因/基因ID:
ACTN2(88) | ACTN3(89)
表达:
P35609 ACTN2_HUMAN:

Expressed in both skeletal and cardiac muscle.

Q08043 ACTN3_HUMAN:

Expressed only in a subset of type 2 skeletal muscle fibers.

序列:
MNQIEPGVQYNYVYDEDEYMIQEEEWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRNGLKLMLLLEVISGERLPKPDRGKMRFHKIANVNKALDYIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYRNVNIQNFHTSWKDGLGLCALIHRHRPDLIDYSKLNKDDPIGNINLAMEIAEKHLDIPKMLDAEDIVNTPKPDERAIMTYVSCFYHAFAGAEQAETAANRICKVLAVNQENERLMEEYERLASELLEWIRRTIPWLENRTPEKTMQAMQKKLEDFRDYRRKHKPPKVQEKCQLEINFNTLQTKLRISNRPAFMPSEGKMVSDIAGAWQRLEQAEKGYEEWLLNEIRRLERLEHLAEKFRQKASTHETWAYGKEQILLQKDYESASLTEVRALLRKHEAFESDLAAHQDRVEQIAAIAQELNELDYHDAVNVNDRCQKICDQWDRLGTLTQKRREALERMEKLLETIDQLHLEFAKRAAPFNNWMEGAMEDLQDMFIVHSIEEIQSLITAHEQFKATLPEADGERQSIMAIQNEVEKVIQSYNIRISSSNPYSTVTMDELRTKWDKVKQLVPIRDQSLQEELARQHANERLRRQFAAQANAIGPWIQNKMEEIARSSIQITGALEDQMNQLKQYEHNIINYKNNIDKLEGDHQLIQEALVFDNKHTNYTMEHIRVGWELLLTTIARTINEVETQILTRDAKGITQEQMNEFRASFNHFDRRKNGLMDHEDFRACLISMGYDLGEAEFARIMTLVDPNGQGTVTFQSFIDFMTRETADTDTAEQVIASFRILASDKPYILAEELRRELPPDQAQYCIKRMPAYSGPGSVPGALDYAAFSSALYGESDL

MMMVMQPEGLGAGEGRFAGGGGGGEYMEQEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRNGLKLMLLLEVISGERLPRPDKGKMRFHKIANVNKALDFIASKGVKLVSIGAEEIVDGNLKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYRNVNVQNFHTSWKDGLALCALIHRHRPDLIDYAKLRKDDPIGNLNTAFEVAEKYLDIPKMLDAEDIVNTPKPDEKAIMTYVSCFYHAFAGAEQAETAANRICKVLAVNQENEKLMEEYEKLASELLEWIRRTVPWLENRVGEPSMSAMQRKLEDFRDYRRLHKPPRIQEKCQLEINFNTLQTKLRLSHRPAFMPSEGKLVSDIANAWRGLEQVEKGYEDWLLSEIRRLQRLQHLAEKFRQKASLHEAWTRGKEEMLSQRDYDSALLQEVRALLRRHEAFESDLAAHQDRVEHIAALAQELNELDYHEAASVNSRCQAICDQWDNLGTLTQKRRDALERMEKLLETIDRLQLEFARRAAPFNNWLDGAVEDLQDVWLVHSVEETQSLLTAHDQFKATLPEADRERGAIMGIQGEIQKICQTYGLRPCSTNPYITLSPQDINTKWDMVRKLVPSCDQTLQEELARQQVNERLRRQFAAQANAIGPWIQAKVEEVGRLAAGLAGSLEEQMAGLRQQEQNIINYKTNIDRLEGDHQLLQESLVFDNKHTVYSMEHIRVGWEQLLTSIARTINEVENQVLTRDAKGLSQEQLNEFRASFNHFDRKQNGMMEPDDFRACLISMGYDLGEVEFARIMTMVDPNAAGVVTFQAFIDFMTRETAETDTTEQVVASFKILAGDKNYITPEELRRELPAKQAEYCIRRMVPYKGSGAPAGALDYVAFSSALYGESDL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
88
Pig
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P35609/Q08043 作为底物

Site PTM Type Enzyme
K38 Ubiquitination
K42 Ubiquitination
K54 Ubiquitination
T57 Phosphorylation
R83 Methylation
K86 Methylation
Y106 Phosphorylation
S116 Phosphorylation
T130 Phosphorylation
S147 Phosphorylation
T151 Phosphorylation
S152 Phosphorylation
K181 Acetylation
Y200 Phosphorylation
S201 Phosphorylation
T237 Phosphorylation
S250 Phosphorylation
Y253 Phosphorylation
R268 Methylation
S291 Phosphorylation
Y326 Phosphorylation
K338 Ubiquitination
T347 Phosphorylation
T350 Phosphorylation
S355 Phosphorylation
Y385 Phosphorylation
S431 Phosphorylation
S433 Phosphorylation
T435 Phosphorylation
K443 Acetylation
K443 Ubiquitination
S449 Phosphorylation
K499 Acetylation
K523 Acetylation
S588 Phosphorylation
Y589 Phosphorylation
S594 Phosphorylation
S595 Phosphorylation
S596 Phosphorylation
Y599 Phosphorylation
Y681 Phosphorylation
Y715 Phosphorylation
S840 Phosphorylation
Y844 Phosphorylation
Y861 Phosphorylation
S892 Phosphorylation
Site PTM Type Enzyme
M1 Acetylation
K45 Ubiquitination
K49 Ubiquitination
K61 Ubiquitination
T64 Phosphorylation
R90 Methylation
R93 Methylation
S116 Phosphorylation
K117 Ubiquitination
T137 Phosphorylation
S154 Phosphorylation
T158 Phosphorylation
S159 Phosphorylation
K209 Sumoylation
K212 Sumoylation
Y229 Phosphorylation
K250 Acetylation
S257 Phosphorylation
Y260 Phosphorylation
R275 Methylation
K278 Acetylation
S298 Phosphorylation
S319 Phosphorylation
S321 Phosphorylation
Y333 Phosphorylation
T354 Phosphorylation
T357 Phosphorylation
S376 Phosphorylation
S418 Phosphorylation
Y436 Phosphorylation
S438 Phosphorylation
S456 Phosphorylation
K506 Acetylation
S737 Phosphorylation
Y851 Phosphorylation
Y876 Phosphorylation

研究背景

功能:

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.

翻译修饰:

Ubiquitinated by FBXL22, leading to proteasomal degradation.

细胞定位:

Cytoplasm>Myofibril>Sarcomere>Z line.
Note: Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in both skeletal and cardiac muscle.

亚基结构:

Homodimer; antiparallel. Also forms heterodimers with ACTN3. Interacts with ADAM12, MYOZ1, MYOZ2 and MYOZ3. Interacts via its C-terminal region with the LDB3 PDZ domain. Interacts with XIRP2. Interacts with DST isoform 1 (via N-terminus). Interacts with PARVB. Interacts with SYNPO2.

蛋白家族:

Belongs to the alpha-actinin family.

功能:

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.

组织特异性:

Expressed only in a subset of type 2 skeletal muscle fibers.

亚基结构:

Homodimer; antiparallel. Also forms heterodimers with ACTN2. Interacts with MYOZ1.

蛋白家族:

Belongs to the alpha-actinin family.

研究领域

· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).

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