PFKFB1/4 Antibody - #DF3307

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产品: PFKFB1/4 抗体
货号: DF3307
描述: Rabbit polyclonal antibody to PFKFB1/4
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 54 KD; 55kD,54kD(Calculated).
蛋白号: P16118 | Q16877
RRID: AB_2835675

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
PFKFB1/4 Antibody detects endogenous levels of total PFKFB1/4.
RRID:
AB_2835675
引用格式: Affinity Biosciences Cat# DF3307, RRID:AB_2835675.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

6 phosphofructo 2 kinase/ fructose 2,6 bisphosphatase; 6 phosphofructo 2 kinase/fructose 2,6 biphosphatase 3; 6-bisphosphatase; 6-P2ase 3; 6-P2ASE brain/placenta-type isozyme; 6PF 2 K/Fru 2,6 P2ASE brain/placenta type isozyme; 6PF 2-K/Fru 2,6 P2ase 3; 6PF-2-K/Fru-2; F263_HUMAN; fructose 6 phosphate,2 kinase/fructose 2, 6 bisphosphatase; Fructose-2; Inducible 6 phosphofructo 2 kinase/fructose 2,6 bisphosphatase; iPFK 2; iPFK-2; IPFK2; PFK/FBPase 3; PFK2; PFKFB3; Renal carcinoma antigen NY REN 56; Renal carcinoma antigen NY-REN-56; uPFK 2; 6 phosphofructo 2 kinase/ fructose 2,6 bisphosphatase; 6 phosphofructo 2 kinase/fructose 2,6 biphosphatase 3; 6-bisphosphatase; 6-P2ase 3; 6-P2ASE brain/placenta-type isozyme; 6PF 2 K/Fru 2,6 P2ASE brain/placenta type isozyme; 6PF 2-K/Fru 2,6 P2ase 3; 6PF-2-K/Fru-2; F263_HUMAN; fructose 6 phosphate,2 kinase/fructose 2, 6 bisphosphatase; Fructose-2; Inducible 6 phosphofructo 2 kinase/fructose 2,6 bisphosphatase; iPFK 2; iPFK-2; IPFK2; PFK/FBPase 3; PFK2; PFKFB3; Renal carcinoma antigen NY REN 56; Renal carcinoma antigen NY-REN-56; uPFK 2;

抗原和靶标

免疫原:
Uniprot:

P16118(F261_HUMAN) >>Visit HPA database.

Q16877(F264_HUMAN) >>Visit HPA database.

基因/基因ID:
PFKFB1(5207) | PFKFB4(5210)
表达:
P16118 F261_HUMAN:

Liver.

序列:
MSPEMGELTQTRLQKIWIPHSSGSSRLQRRRGSSIPQFTNSPTMVIMVGLPARGKTYISTKLTRYLNWIGTPTKVFNLGQYRREAVSYKNYEFFLPDNMEALQIRKQCALAALKDVHNYLSHEEGHVAVFDATNTTRERRSLILQFAKEHGYKVFFIESICNDPGIIAENIRQVKLGSPDYIDCDREKVLEDFLKRIECYEVNYQPLDEELDSHLSYIKIFDVGTRYMVNRVQDHIQSRTVYYLMNIHVTPRSIYLCRHGESELNIRGRIGGDSGLSVRGKQYAYALANFIQSQGISSLKVWTSHMKRTIQTAEALGVPYEQWKALNEIDAGVCEEMTYEEIQEHYPEEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKSSDELPYLKCPLHTVLKLTPVAYGCKVESIYLNVEAVNTHREKPENVDITREPEEALDTVPAHY

MASPRELTQNPLKKIWMPYSNGRPALHACQRGVCMTNCPTLIVMVGLPARGKTYISKKLTRYLNWIGVPTREFNVGQYRRDVVKTYKSFEFFLPDNEEGLKIRKQCALAALRDVRRFLSEEGGHVAVFDATNTTRERRATIFNFGEQNGYKTFFVESICVDPEVIAANIVQVKLGSPDYVNRDSDEATEDFMRRIECYENSYESLDEDLDRDLSYIKIMDVGQSYVVNRVADHIQSRIVYYLMNIHVTPRSIYLCRHGESELNLKGRIGGDPGLSPRGREFAKSLAQFISDQNIKDLKVWTSQMKRTIQTAEALGVPYEQWKVLNEIDAGVCEEMTYEEIQDNYPLEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKAAEQLPYLKCPLHTVLKLTPVAYGCKVESIFLNVAAVNTHRDRPQNVDISRPPEEALVTVPAHQ

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Rabbit
100
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P16118/Q16877 作为底物

Site PTM Type Enzyme
S2 Phosphorylation
S24 Phosphorylation
T39 Phosphorylation
T133 Phosphorylation
Y200 Phosphorylation
Y204 Phosphorylation
Y227 Phosphorylation
Y242 Phosphorylation
Y243 Phosphorylation
S262 Phosphorylation
K364 Ubiquitination
Y438 Phosphorylation
Site PTM Type Enzyme
S3 Phosphorylation
K13 Ubiquitination
K87 Ubiquitination
K101 Ubiquitination
K104 Ubiquitination
S119 Phosphorylation
T131 Phosphorylation
S176 Phosphorylation
S214 Phosphorylation
K265 Ubiquitination
S275 Phosphorylation
K283 Ubiquitination
K295 Ubiquitination
K305 Ubiquitination
K362 Ubiquitination

研究背景

功能:

Synthesis and degradation of fructose 2,6-bisphosphate.

组织特异性:

Liver.

亚基结构:

Homodimer.

蛋白家族:

In the C-terminal section; belongs to the phosphoglycerate mutase family.

功能:

Synthesis and degradation of fructose 2,6-bisphosphate.

亚基结构:

Homodimer.

蛋白家族:

In the C-terminal section; belongs to the phosphoglycerate mutase family.

研究领域

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Metabolism > Carbohydrate metabolism > Fructose and mannose metabolism.

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

文献引用

1). Silencing of PFKFB3 protects podocytes against high glucose‑induced injury by inducing autophagy. Molecular Medicine Reports (PubMed: 34490476) [IF=3.4]

Application: WB    Species: Mouse    Sample: MPC5 cells

Figure 1. AR and PFKFB3 siRNAs are successfully transfected into MPC5 cells. MPC5 cells were treated with HG for 48 or 72 h. (A) Protein expression levels of enolase-1, PFKFB3, glyoxalase 1 and AR in MPC5 cells were detected by western blot analysis and normalized to GAPDH. (B) MPC5 cells were transfected with siRNA NC, siRNA AR or PFKFB3 siRNA1, −2 or −3 for 24 h. Then, the protein expression levels of PFKFB3 and AR were measured by western blot analysis and normalized to GAPDH. **P<0.01 vs. Control (NG). AR, aldose reductase; HG, high glucose; NC, negative control; PFKFB3, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3; siRNA, small interfering RNA.

Application: WB    Species: mouse    Sample: MPC5 cells

Figure 4.| Knockdown of PFKFB3 reverses podocyte injury through inhibition of the epithelial‑mesenchymal transition process. Protein expression levels of(A) PFKFB3, (B) WT‑1 and (C) E‑cadherin and α‑SMA in MPC5 cells were determined by western blot analysis and normalized to GAPDH.

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