LAMC1 Antibody - #DF3619

(1)   (1)  
产品: LAMC1 抗体
货号: DF3619
描述: Rabbit polyclonal antibody to LAMC1
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Horse, Sheep, Dog
分子量: 178 KD; 178kD(Calculated).
蛋白号: P11047
RRID: AB_2835991

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(88%), Horse(100%), Sheep(100%), Dog(100%)
克隆:
Polyclonal
特异性:
LAMC1 Antibody detects endogenous levels of total LAMC1.
RRID:
AB_2835991
引用格式: Affinity Biosciences Cat# DF3619, RRID:AB_2835991.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

LAMB2; lamc1; LAMC1_HUMAN; Laminin B2 chain; Laminin subunit gamma-1; Laminin, gamma 1 (formerly LAMB2); Laminin-1 subunit gamma; Laminin-10 subunit gamma; Laminin-11 subunit gamma; Laminin-2 subunit gamma; Laminin-3 subunit gamma; Laminin-4 subunit gamma; Laminin-6 subunit gamma; Laminin-7 subunit gamma; Laminin-8 subunit gamma; Laminin-9 subunit gamma; MGC87297; S-LAM gamma; S-laminin subunit gamma;

抗原和靶标

免疫原:
Uniprot:

P11047(LAMC1_HUMAN) >>Visit HPA database.

基因/基因ID:
LAMC1(3915)
表达:
P11047 LAMC1_HUMAN:

Found in the basement membranes (major component).

序列:
MRGSHRAAPALRPRGRLWPVLAVLAAAAAAGCAQAAMDECTDEGGRPQRCMPEFVNAAFNVTVVATNTCGTPPEEYCVQTGVTGVTKSCHLCDAGQPHLQHGAAFLTDYNNQADTTWWQSQTMLAGVQYPSSINLTLHLGKAFDITYVRLKFHTSRPESFAIYKRTREDGPWIPYQYYSGSCENTYSKANRGFIRTGGDEQQALCTDEFSDISPLTGGNVAFSTLEGRPSAYNFDNSPVLQEWVTATDIRVTLNRLNTFGDEVFNDPKVLKSYYYAISDFAVGGRCKCNGHASECMKNEFDKLVCNCKHNTYGVDCEKCLPFFNDRPWRRATAESASECLPCDCNGRSQECYFDPELYRSTGHGGHCTNCQDNTDGAHCERCRENFFRLGNNEACSSCHCSPVGSLSTQCDSYGRCSCKPGVMGDKCDRCQPGFHSLTEAGCRPCSCDPSGSIDECNIETGRCVCKDNVEGFNCERCKPGFFNLESSNPRGCTPCFCFGHSSVCTNAVGYSVYSISSTFQIDEDGWRAEQRDGSEASLEWSSERQDIAVISDSYFPRYFIAPAKFLGKQVLSYGQNLSFSFRVDRRDTRLSAEDLVLEGAGLRVSVPLIAQGNSYPSETTVKYVFRLHEATDYPWRPALTPFEFQKLLNNLTSIKIRGTYSERSAGYLDDVTLASARPGPGVPATWVESCTCPVGYGGQFCEMCLSGYRRETPNLGPYSPCVLCACNGHSETCDPETGVCNCRDNTAGPHCEKCSDGYYGDSTAGTSSDCQPCPCPGGSSCAVVPKTKEVVCTNCPTGTTGKRCELCDDGYFGDPLGRNGPVRLCRLCQCSDNIDPNAVGNCNRLTGECLKCIYNTAGFYCDRCKDGFFGNPLAPNPADKCKACNCNLYGTMKQQSSCNPVTGQCECLPHVTGQDCGACDPGFYNLQSGQGCERCDCHALGSTNGQCDIRTGQCECQPGITGQHCERCEVNHFGFGPEGCKPCDCHPEGSLSLQCKDDGRCECREGFVGNRCDQCEENYFYNRSWPGCQECPACYRLVKDKVADHRVKLQELESLIANLGTGDEMVTDQAFEDRLKEAEREVMDLLREAQDVKDVDQNLMDRLQRVNNTLSSQISRLQNIRNTIEETGNLAEQARAHVENTERLIEIASRELEKAKVAAANVSVTQPESTGDPNNMTLLAEEARKLAERHKQEADDIVRVAKTANDTSTEAYNLLLRTLAGENQTAFEIEELNRKYEQAKNISQDLEKQAARVHEEAKRAGDKAVEIYASVAQLSPLDSETLENEANNIKMEAENLEQLIDQKLKDYEDLREDMRGKELEVKNLLEKGKTEQQTADQLLARADAAKALAEEAAKKGRDTLQEANDILNNLKDFDRRVNDNKTAAEEALRKIPAINQTITEANEKTREAQQALGSAAADATEAKNKAHEAERIASAVQKNATSTKAEAERTFAEVTDLDNEVNNMLKQLQEAEKELKRKQDDADQDMMMAGMASQAAQEAEINARKAKNSVTSLLSIINDLLEQLGQLDTVDLNKLNEIEGTLNKAKDEMKVSDLDRKVSDLENEAKKQEAAIMDYNRDIEEIMKDIRNLEDIRKTLPSGCFNTPSIEKP

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Zebrafish
88
Xenopus
75
Chicken
75
Bovine
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P11047 作为底物

Site PTM Type Enzyme
S4 Phosphorylation
S159 Phosphorylation
K164 Ubiquitination
T166 Phosphorylation
S179 Phosphorylation
S181 Phosphorylation
T185 Phosphorylation
S187 Phosphorylation
K188 Ubiquitination
T196 Phosphorylation
S210 Phosphorylation
T216 Phosphorylation
T258 Phosphorylation O14757 (CHEK1)
K268 Ubiquitination
S272 Phosphorylation
Y273 Phosphorylation
Y274 Phosphorylation
Y275 Phosphorylation
K302 Ubiquitination
K308 Ubiquitination
Y413 Phosphorylation
N650 N-Glycosylation
S664 Phosphorylation
Y667 Phosphorylation
S675 Phosphorylation
K788 Ubiquitination
K802 Ubiquitination
K1093 Ubiquitination
R1102 Methylation
N1107 N-Glycosylation
T1127 Phosphorylation
S1149 Phosphorylation
N1161 N-Glycosylation
N1175 N-Glycosylation
N1223 N-Glycosylation
N1241 N-Glycosylation
S1275 Phosphorylation
K1303 Ubiquitination
Y1307 Phosphorylation
K1329 Ubiquitination
K1371 Ubiquitination
N1395 N-Glycosylation
K1404 Acetylation
K1466 Ubiquitination
S1493 Phosphorylation
T1529 Phosphorylation
T1541 Phosphorylation
K1544 Ubiquitination
K1557 Ubiquitination
S1559 Phosphorylation
K1566 Ubiquitination
K1584 Ubiquitination
T1603 O-Glycosylation
S1605 Phosphorylation

研究背景

功能:

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

细胞定位:

Secreted>Extracellular space>Extracellular matrix>Basement membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Found in the basement membranes (major component).

亚基结构:

Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Gamma-1 is a subunit of laminin-1 (laminin-111 or EHS laminin), laminin-2 (laminin-211 or merosin), laminin-3 (laminin-121 or S-laminin), laminin-4 (laminin-221 or S-merosin), laminin-6 (laminin-311 or K-laminin), laminin-7 (laminin-321 or KS-laminin), laminin-8 (laminin-411), laminin-9 (laminin-421), laminin-10 (laminin-511) and laminin-11 (laminin-521).

蛋白家族:

The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.

Domains VI and IV are globular.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > ECM-receptor interaction.   (View pathway)

· Human Diseases > Neurodegenerative diseases > Prion diseases.

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

文献引用

1). miRNA-96-5p alleviates liver fibrosis by targeting FN1 and inhibiting ECM-receptor interaction pathway. Research Square

Application: WB    Species: Mouse    Sample:

Fig. 2FN1 promotes the activation of HSCs.aHeatmap of DEMs in ECM-receptor inter-action pathway.bThe expressions of the Top 10 different kinds of up-regulatedDEMs andα-SMA in TGF-β1 activated HSC line LX-2 cells and in inactivated HSCs were evaluatedqRT-PCR analysis.cThe protein levels of the Top 3 up-regulated genes verified byqRT-PCR andα-SMA were evaluated by Western blotting.dThe expression ofα-SMA, COLAL1,FN1, and LAMC1 in TGF-β1 activated HSCs transfected with FN1-siRNA and cells trans-fected with Control siRNA were evaluated qRT-PCR analysis.eThe expressions ofα-SMA,COLAL1, FN1, and LAMC1 in TGF-β1 activated HSCs transfected with FN1-siRNA andcells transfected with Control siRNA were evaluated by Western blotting

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.