产品: RPB11 抗体
货号: DF4003
描述: Rabbit polyclonal antibody to RPB11
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Sheep, Dog, Chicken, Xenopus
分子量: 13 KD; 13kD(Calculated).
蛋白号: P52435
RRID: AB_2836363

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
RPB11 Antibody detects endogenous levels of total RPB11.
RRID:
AB_2836363
引用格式: Affinity Biosciences Cat# DF4003, RRID:AB_2836363.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

DNA directed RNA polymerase II subunit RPB11 a; DNA-directed RNA polymerase II 13.3 kDa polypeptide; DNA-directed RNA polymerase II 13.3 kDa polypeptide; DNA-directed RNA polymerase II subunit J 1; DNA-directed RNA polymerase II subunit J-1; DNA-directed RNA polymerase II subunit RPB11-a; hRPB 14; hRPB14; POLR 2J1; POLR2J; POLR2J1; polymerase (RNA) II (DNA directed) polypeptide J 13.3kDa; polymerase (RNA) II (DNA directed) polypeptide J 13.3kDa; RNA polymerase II 13.3 kDa subunit; RNA polymerase II subunit B11 a; RNA polymerase II subunit B11-a; RPB 11; RPB 11A; RPB 11m; RPB11; RPB11_HUMAN; RPB11a; RPB11m; Rpo2-4;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
P52435 RPB11_HUMAN:

Ubiquitously expressed. High expression was found in heart and skeletal muscle.

序列:
MNAPPAFESFLLFEGEKKITINKDTKVPNACLFTINKEDHTLGNIIKSQLLKDPQVLFAGYKVPHPLEHKIIIRVQTTPDYSPQEAFTNAITDLISELSLLEERFRVAIKDKQEGIE

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Horse
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P52435 作为底物

Site PTM Type Enzyme
K23 Ubiquitination
K26 Ubiquitination
K37 Ubiquitination
T41 Phosphorylation
K47 Ubiquitination
K52 Ubiquitination
Y61 Phosphorylation
K62 Ubiquitination
K70 Ubiquitination
K110 Ubiquitination

研究背景

功能:

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB11 is part of the core element with the central large cleft (By similarity).

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitously expressed. High expression was found in heart and skeletal muscle.

亚基结构:

Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. Interacts with AATF.

蛋白家族:

Belongs to the archaeal RpoL/eukaryotic RPB11/RPC19 RNA polymerase subunit family.

研究领域

· Genetic Information Processing > Transcription > RNA polymerase.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

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