CYB5R3 Antibody - #DF4212

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产品: CYB5R3 抗体
货号: DF4212
描述: Rabbit polyclonal antibody to CYB5R3
应用: WB IHC IF/ICC
反应: Human, Mouse
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 34 KD; 34kD(Calculated).
蛋白号: P00387
RRID: AB_2836563

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MS Report
HPLC Report
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(100%), Bovine(90%), Horse(100%), Sheep(90%), Rabbit(90%), Dog(90%)
克隆:
Polyclonal
特异性:
CYB5R3 Antibody detects endogenous levels of total CYB5R3.
RRID:
AB_2836563
引用格式: Affinity Biosciences Cat# DF4212, RRID:AB_2836563.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

B5R; Cyb5r3; Cytochrome b5 reductase 3; Cytochrome b5 reductase; DIA1; Diaphorase 1; Diaphorase-1; NADH cytochrome b5 reductase 3; NADH-cytochrome b5 reductase 3 membrane-bound form; NADH-cytochrome b5 reductase 3 soluble form; NB5R3_HUMAN; OTTHUMP00000028761; OTTHUMP00000198435; OTTHUMP00000198574; OTTHUMP00000198662; OTTHUMP00000198665;

抗原和靶标

免疫原:

A synthesized peptide derived from human CYB5R3, corresponding to a region within C-terminal amino acids.

Uniprot:

P00387(NB5R3_HUMAN) >>Visit HPA database.

基因/基因ID:
CYB5R3(1727)
表达:
P00387 NB5R3_HUMAN:

Isoform 2 is expressed at late stages of erythroid maturation.

序列:
MGAQLSTLGHMVLFPVWFLYSLLMKLFQRSTPAITLESPDIKYPLRLIDREIISHDTRRFRFALPSPQHILGLPVGQHIYLSARIDGNLVVRPYTPISSDDDKGFVDLVIKVYFKDTHPKFPAGGKMSQYLESMQIGDTIEFRGPSGLLVYQGKGKFAIRPDKKSNPIIRTVKSVGMIAGGTGITPMLQVIRAIMKDPDDHTVCHLLFANQTEKDILLRPELEELRNKHSARFKLWYTLDRAPEAWDYGQGFVNEEMIRDHLPPPEEEPLVLMCGPPPMIQYACLPNLDHVGHPTERCFVF

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
90
Sheep
90
Dog
90
Rabbit
90
Zebrafish
63
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P00387 作为底物

Site PTM Type Enzyme
G2 Myristoylation
S21 Phosphorylation
T31 Phosphorylation
S38 Phosphorylation
K42 Acetylation
K42 Ubiquitination
Y43 Phosphorylation
S66 Phosphorylation
Y80 Phosphorylation
S82 Phosphorylation
T95 Phosphorylation
K115 Ubiquitination
K120 Ubiquitination
K126 Ubiquitination
S146 Phosphorylation
K154 Ubiquitination
K163 Ubiquitination
K164 Ubiquitination
T171 Phosphorylation
K173 Ubiquitination
S174 Phosphorylation
T182 Phosphorylation
T185 Phosphorylation
K196 Acetylation
K214 Ubiquitination
K234 Ubiquitination
Y248 Phosphorylation

研究背景

功能:

Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.

细胞定位:

Endoplasmic reticulum membrane>Lipid-anchor>Cytoplasmic side. Mitochondrion outer membrane>Lipid-anchor>Cytoplasmic side.

Cytoplasm.
Note: Produces the soluble form found in erythrocytes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Isoform 2 is expressed at late stages of erythroid maturation.

亚基结构:

Component of a complex composed of cytochrome b5, NADH-cytochrome b5 reductase (CYB5R3) and MTARC2.

蛋白家族:

Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.

研究领域

· Metabolism > Carbohydrate metabolism > Amino sugar and nucleotide sugar metabolism.

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