ARHGEF12 Antibody - #DF4432

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产品: ARHGEF12 抗体
货号: DF4432
描述: Rabbit polyclonal antibody to ARHGEF12
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 173 KD; 173kD(Calculated).
蛋白号: Q9NZN5
RRID: AB_2836787

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MSDS
说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%)
克隆:
Polyclonal
特异性:
ARHGEF12 Antibody detects endogenous levels of total ARHGEF12.
RRID:
AB_2836787
引用格式: Affinity Biosciences Cat# DF4432, RRID:AB_2836787.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ARHGC_HUMAN; ARHGEF12; KIAA0382; leukemia-associated rho guanine nucleotide exchange factor; Leukemia-associated RhoGEF; PRO2792; Rho guanine exchange factor (GEF) 12; Rho guanine nucleotide exchange factor (GEF) 12; Rho guanine nucleotide exchange factor 12; Similar to Mouse Lsc oncogene;

抗原和靶标

免疫原:
Uniprot:

Q9NZN5(ARHGC_HUMAN) >>Visit HPA database.

基因/基因ID:
ARHGEF12(23365)
表达:
Q9NZN5 ARHGC_HUMAN:

Ubiquitously expressed. Isoform 2 is found in jejunum and testis.

序列:
MSGTQSTITDRFPLKKPIRHGSILNRESPTDKKQKVERIASHDFDPTDSSSKKTKSSSEESRSEIYGLVQRCVIIQKDDNGFGLTVSGDNPVFVQSVKEDGAAMRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIKSGSYVALTVQGRPPGSPQIPLADSEVEPSVIGHMSPIMTSPHSPGASGNMERITSPVLMGEENNVVHNQKVEILRKMLQKEQERLQLLQEDYNRTPAQRLLKEIQEAKKHIPQLQEQLSKATGSAQDGAVVTPSRPLGDTLTVSEAETDPGDVLGRTDCSSGDASRPSSDNADSPKSGPKERIYLEENPEKSETIQDTDTQSLVGSPSTRIAPHIIGAEDDDFGTEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYKHTNSKETRRIFLEFHQFFLDRSAHLKVSVPDEMSADLEKRRPELIPEDLHRHYIQTMQERVHPEVQRHLEDFRQKRSMGLTLAESELTKLDAERDKDRLTLEKERTCAEQIVAKIEEVLMTAQAVEEDKSSTMQYVILMYMKHLGVKVKEPRNLEHKRGRIGFLPKIKQSMKKDKEGEEKGKRRGFPSILGPPRRPSRHDNSAIGRAMELQKARHPKHLSTPSSVSPEPQDSAKLRQSGLANEGTDAGYLPANSMSSVASGASFSQEGGKENDTGSKQVGETSAPGDTLDGTPRTLNTVFDFPPPPLDQVQEEECEVERVTEHGTPKPFRKFDSVAFGESQSEDEQFENDLETDPPNWQQLVSREVLLGLKPCEIKRQEVINELFYTERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHIGLNEQMKAVRKRNETSVIDQIGEDLLTWFSGPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFQTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPTEREKVKKAADHCRQILNYVNQAVKEAENKQRLEDYQRRLDTSSLKLSEYPNVEELRNLDLTKRKMIHEGPLVWKVNRDKTIDLYTLLLEDILVLLQKQDDRLVLRCHSKILASTADSKHTFSPVIKLSTVLVRQVATDNKALFVISMSDNGAQIYELVAQTVSEKTVWQDLICRMAASVKEQSTKPIPLPQSTPGEGDNDEEDPSKLKEEQHGISVTGLQSPDRDLGLESTLISSKPQSHSLSTSGKSEVRDLFVAERQFAKEQHTDGTLKEVGEDYQIAIPDSHLPVSEERWALDALRNLGLLKQLLVQQLGLTEKSVQEDWQHFPRYRTASQGPQTDSVIQNSENIKAYHSGEGHMPFRTGTGDIATCYSPRTSTESFAPRDSVGLAPQDSQASNILVMDHMIMTPEMPTMEPEGGLDDSGEHFFDAREAHSDENPSEGDGAVNKEEKDVNLRISGNYLILDGYDPVQESSTDEEVASSLTLQPMTGIPAVESTHQQQHSPQNTHSDGAISPFTPEFLVQQRWGAMEYSCFEIQSPSSCADSQSQIMEYIHKIEADLEHLKKVEESYTILCQRLAGSALTDKHSDKS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
92
Xenopus
64
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9NZN5 作为底物

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
T9 Phosphorylation
R11 Methylation
S22 Phosphorylation
S28 Phosphorylation
T30 Phosphorylation
K32 Acetylation
S41 Phosphorylation
Y66 Phosphorylation
S178 Phosphorylation
S190 Phosphorylation
K205 Ubiquitination
C294 S-Nitrosylation
S296 Phosphorylation
S304 Phosphorylation
S309 Phosphorylation
S312 Phosphorylation
S337 Phosphorylation
S341 Phosphorylation
T532 Phosphorylation
Y546 Phosphorylation
K591 Acetylation
K593 Acetylation
S599 Phosphorylation
S608 Phosphorylation
S613 Phosphorylation
R617 Methylation
T632 Phosphorylation
S634 Phosphorylation
S635 Phosphorylation
S637 Phosphorylation
Y660 Phosphorylation
T699 Phosphorylation
T703 Phosphorylation
T732 Phosphorylation
T736 Phosphorylation
S753 Phosphorylation
S818 Phosphorylation
S824 Phosphorylation
S826 Phosphorylation
R829 Methylation
C921 S-Nitrosylation
K1000 Ubiquitination
Y1004 Phosphorylation
K1017 Ubiquitination
K1064 Ubiquitination
S1072 Phosphorylation
S1170 Phosphorylation
S1176 Phosphorylation
S1203 Phosphorylation
Y1232 Phosphorylation
S1239 Phosphorylation
S1273 Phosphorylation
Y1284 Phosphorylation
T1286 Phosphorylation
S1288 Phosphorylation
T1293 Phosphorylation
S1295 Phosphorylation
S1308 Phosphorylation
T1324 Phosphorylation
Y1326 Phosphorylation
S1327 Phosphorylation
S1331 Phosphorylation
S1377 Phosphorylation
S1389 Phosphorylation
Y1415 Phosphorylation
S1457 Phosphorylation
S1463 Phosphorylation
S1492 Phosphorylation
Y1524 Phosphorylation
T1525 Phosphorylation
S1541 Phosphorylation
S1544 Phosphorylation

研究背景

功能:

May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13.

细胞定位:

Cytoplasm. Membrane.
Note: Translocated to the membrane upon stimulation.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitously expressed. Isoform 2 is found in jejunum and testis.

亚基结构:

Interacts with GNA12 and GNA13, probably through the RGS-like domain. Interacts with RHOA, PLXNB1 and PLXNB2. Interacts through its PDZ domain with IGF1R beta subunit. Interacts with GCSAM.

研究领域

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

· Organismal Systems > Development > Axon guidance.   (View pathway)

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

文献引用

1). ITRAQ-Based Proteomics Analysis Reveals the Effect of Neoliensinine on KCl-Induced Vascular Smooth Muscle Contraction by Inhibiting Regulatory Light Chain Phosphorylation. Frontiers in Pharmacology (PubMed: 31572175) [IF=5.6]

Application: WB    Species: mouse    Sample: mesenteric arteries

FIGURE 4 | Reducing expressions of PLCβ and RhoGEF12 of neoliensinine in mesenteric arteries contracted by KCl. (A) Protein expressions of PLCβ and RhoGEF12 by Western blot
2). MiR-361-5p/abca1 and MiR-196-5p/arhgef12 Axis Involved in γ-Sitosterol Inducing Dual Anti-Proliferative Effects on Bronchial Epithelial Cells of Chronic Obstructive Pulmonary Disease. International Journal of Chronic Obstructive Pulmonary Disease (PubMed: 34675500) [IF=2.8]

Application: WB    Species: Human    Sample: H292 and BEAS-2B cells

Figure 3 Effect of GS on regulating miR-196-5p and miR-361-5p expression. (A) After GS treatment, the expressions of miR-196-5p and miR-361-5p were detected in BEAS-2B and H292 cells. (B) Cell proliferation were performed in BEAS-2B cells after GS single or combined with either miR-196-5p mimic or miR-361-5p inhibitor. (C) miR-196-5p and miR-361-5p target mRNAs were matched to GSE130928 profile, respectively. The expressions of bcat1 and arhgef12 of GSE130928 were showed in (D) and the binding motifs of miR-196-5p to arhgef12 and miR-361-5p to bcat1 were showed in (E), respectively. The mRNA (F) and protein levels (G) were detected in H292 and BEAS-2B cells. Shown are representative and expressed as the Means ± S.D. The bars with different superscripts in each panel were significantly different. Experiments were performed in triplicate for each group, *p< 0.05.

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