(1)
(4)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4449, RRID:AB_2836804.
展开/折叠
fast twitch skeletal muscle isoform; AT2A1_HUMAN; ATP2A; ATP2A1; ATPase Ca++ transporting cardiac muscle fast twitch 1; ATPase Ca++ transporting fast twitch 1; ATPase, Ca(2+)-transporting fast twitch 1; Calcium pump 1; Calcium transporting ATPase sarcoplasmic reticulum type fast twitch skeletal muscle isoform; Calcium-transporting ATPase sarcoplasmic reticulum type; EC 3.6.3.8; Endoplasmic reticulum class 1/2 Ca(2+) ATPase; Fast skeletal muscle SR calcium ATPase; OTTHUMP00000162561; OTTHUMP00000162562; Sarcoendoplasmic reticulum calcium ATPase; Sarcoplasmic reticulum Ca(2+)-ATPase 1; Sarcoplasmic/endoplasmic reticulum calcium ATPase 1; SERCA 1; SERCA1; SERCA1 truncated isoform, included; SR Ca(2+) ATPase 1; SR Ca(2+)-ATPase 1;
抗原和靶标
- O14983 AT2A1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEAAHAKTTEECLAYFGVSETTGLTPDQVKRNLEKYGLNELPAEEGKTLWELVIEQFEDLLVRILLLAACISFVLAWFEEGEETITAFVEPFVILLILIANAIVGVWQERNAENAIEALKEYEPEMGKVYRADRKSVQRIKARDIVPGDIVEVAVGDKVPADIRILAIKSTTLRVDQSILTGESVSVIKHTEPVPDPRAVNQDKKNMLFSGTNIAAGKALGIVATTGVGTEIGKIRDQMAATEQDKTPLQQKLDEFGEQLSKVISLICVAVWLINIGHFNDPVHGGSWFRGAIYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVCKMFIIDKVDGDICLLNEFSITGSTYAPEGEVLKNDKPVRPGQYDGLVELATICALCNDSSLDFNEAKGVYEKVGEATETALTTLVEKMNVFNTDVRSLSKVERANACNSVIRQLMKKEFTLEFSRDRKSMSVYCSPAKSSRAAVGNKMFVKGAPEGVIDRCNYVRVGTTRVPLTGPVKEKIMAVIKEWGTGRDTLRCLALATRDTPPKREEMVLDDSARFLEYETDLTFVGVVGMLDPPRKEVTGSIQLCRDAGIRVIMITGDNKGTAIAICRRIGIFGENEEVADRAYTGREFDDLPLAEQREACRRACCFARVEPSHKSKIVEYLQSYDEITAMTGDGVNDAPALKKAEIGIAMGSGTAVAKTASEMVLADDNFSTIVAAVEEGRAIYNNMKQFIRYLISSNVGEVVCIFLTAALGLPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMDRPPRSPKEPLISGWLFFRYMAIGGYVGAATVGAAAWWFLYAEDGPHVNYSQLTHFMQCTEDNTHFEGIDCEVFEAPEPMTMALSVLVTIEMCNALNSLSENQSLLRMPPWVNIWLLGSICLSMSLHFLILYVDPLPMIFKLRALDLTQWLMVLKISLPVIGLDEILKFVARNYLEDPEDERRK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O14983 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S19 | Phosphorylation | Uniprot | |
| K120 | Ubiquitination | Uniprot | |
| K128 | Ubiquitination | Uniprot | |
| S170 | Phosphorylation | Uniprot | |
| S184 | Phosphorylation | Uniprot | |
| S186 | Phosphorylation | Uniprot | |
| K205 | Ubiquitination | Uniprot | |
| K218 | Acetylation | Uniprot | |
| T230 | Phosphorylation | Uniprot | |
| K252 | Ubiquitination | Uniprot | |
| S261 | Phosphorylation | Uniprot | |
| Y294 | Phosphorylation | Uniprot | |
| K328 | Ubiquitination | Uniprot | |
| K329 | Ubiquitination | Uniprot | |
| S335 | Phosphorylation | Uniprot | |
| S338 | Phosphorylation | Uniprot | |
| C344 | S-Nitrosylation | Uniprot | |
| T345 | Phosphorylation | Uniprot | |
| S346 | Phosphorylation | Uniprot | |
| C349 | S-Nitrosylation | Uniprot | |
| S473 | Phosphorylation | Uniprot | |
| K481 | Acetylation | Uniprot | |
| K481 | Ubiquitination | Uniprot | |
| T484 | Phosphorylation | Uniprot | |
| S488 | Phosphorylation | Uniprot | |
| K492 | Acetylation | Uniprot | |
| Y497 | Phosphorylation | Uniprot | |
| K515 | Acetylation | Uniprot | |
| K515 | Ubiquitination | Uniprot | |
| Y527 | Phosphorylation | Uniprot | |
| S581 | Phosphorylation | Uniprot | |
| T608 | Phosphorylation | Uniprot | |
| T625 | Phosphorylation | Uniprot | |
| K684 | Ubiquitination | Uniprot | |
| K713 | Ubiquitination | Uniprot | |
| S722 | Phosphorylation | Uniprot | |
| T724 | Phosphorylation | Uniprot | |
| S731 | Phosphorylation | Uniprot | |
| S741 | Phosphorylation | Uniprot | |
| T742 | Phosphorylation | Uniprot | |
| K758 | Acetylation | Uniprot | |
| K758 | Ubiquitination | Uniprot | |
| S830 | Phosphorylation | Uniprot |
研究背景
Key regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen (By similarity). Contributes to calcium sequestration involved in muscular excitation/contraction.
Endoplasmic reticulum membrane>Multi-pass membrane protein. Sarcoplasmic reticulum membrane>Multi-pass membrane protein.
Skeletal muscle, fast twitch muscle (type II) fibers.
Interacts with sarcolipin (SLN) (By similarity). Interacts with phospholamban (PLN) (By similarity). Interacts with myoregulin (MRLN). Interacts with DWORF (By similarity).
Ca(2+) and ATP binding cause major rearrangements of the cytoplasmic and transmembrane domains. According to the E1-E2 model, Ca(2+) binding to the cytosolic domain of the pump in the high-affinity E1 conformation is followed by the ATP-dependent phosphorylation of the active site Asp, giving rise to E1P. A conformational change of the phosphoenzyme gives rise to the low-affinity E2P state that exposes the Ca(2+) ions to the lumenal side and promotes Ca(2+) release. Dephosphorylation of the active site Asp mediates the subsequent return to the E1 conformation.
PLN and SLN both have a single transmembrane helix; both occupy a similar binding site on ATP2A1 that is situated between the ATP2A1 transmembrane helices.
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.
研究领域
· Environmental Information Processing > Signal transduction > Calcium signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)
· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.
· Organismal Systems > Digestive system > Pancreatic secretion.
文献引用
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