PPP2R5D Antibody - #DF4483

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产品: PPP2R5D 抗体
货号: DF4483
描述: Rabbit polyclonal antibody to PPP2R5D
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Bovine, Horse, Sheep, Rabbit, Dog
分子量: 70 KD; 70kD(Calculated).
蛋白号: Q14738
RRID: AB_2836834

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
克隆:
Polyclonal
特异性:
PPP2R5D Antibody detects endogenous levels of total PPP2R5D.
RRID:
AB_2836834
引用格式: Affinity Biosciences Cat# DF4483, RRID:AB_2836834.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

2A5D_HUMAN; B'delta; B56D; Delta isoform of regulatory subunit B56, protein phosphatase 2A; MGC2134; MGC8949; OTTHUMP00000039821; PP2A B subunit B' delta isoform; PP2A B subunit B56 delta isoform; PP2A B subunit isoform B''-delta; PP2A B subunit isoform B56-delta; PP2A B subunit isoform PR61-delta; PP2A B subunit isoform R5-delta; PP2A B subunit PR61 delta isoform; PP2A B subunit R5 delta isoform; PPP2R5D; Protein phosphatase 2 regulatory subunit B (B56) delta isoform; Protein phosphatase 2 regulatory subunit B delta isoform; Protein phosphatase 2 regulatory subunit B' delta; Serine threonine protein phosphatase 2A 56 kDa regulatory subunit delta isoform; Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform; TEG-271; Tex271;

抗原和靶标

免疫原:
Uniprot:

Q14738(2A5D_HUMAN) >>Visit HPA database.

基因/基因ID:
PPP2R5D(5528)
表达:
Q14738 2A5D_HUMAN:

Isoform Delta-2 is widely expressed. Isoform Delta-1 is highly expressed in brain.

序列:
MPYKLKKEKEPPKVAKCTAKPSSSGKDGGGENTEEAQPQPQPQPQPQAQSQPPSSNKRPSNSTPPPTQLSKIKYSGGPQIVKKERRQSSSRFNLSKNRELQKLPALKDSPTQEREELFIQKLRQCCVLFDFVSDPLSDLKFKEVKRAGLNEMVEYITHSRDVVTEAIYPEAVTMFSVNLFRTLPPSSNPTGAEFDPEEDEPTLEAAWPHLQLVYEFFLRFLESPDFQPNIAKKYIDQKFVLALLDLFDSEDPRERDFLKTILHRIYGKFLGLRAYIRRQINHIFYRFIYETEHHNGIAELLEILGSIINGFALPLKEEHKMFLIRVLLPLHKVKSLSVYHPQLAYCVVQFLEKESSLTEPVIVGLLKFWPKTHSPKEVMFLNELEEILDVIEPSEFSKVMEPLFRQLAKCVSSPHFQVAERALYYWNNEYIMSLISDNAARVLPIMFPALYRNSKSHWNKTIHGLIYNALKLFMEMNQKLFDDCTQQYKAEKQKGRFRMKEREEMWQKIEELARLNPQYPMFRAPPPLPPVYSMETETPTAEDIQLLKRTVETEAVQMLKDIKKEKVLLRRKSELPQDVYTIKALEAHKRAEEFLTASQEAL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Zebrafish
78
Pig
0
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q14738 作为底物

Site PTM Type Enzyme
T18 Phosphorylation
S54 Phosphorylation
S60 Phosphorylation P17612 (PRKACA) , O14757 (CHEK1)
S62 Phosphorylation
T63 Phosphorylation
T67 Phosphorylation
K71 Ubiquitination
K73 Ubiquitination
Y74 Phosphorylation
S75 Phosphorylation P17612 (PRKACA)
K82 Ubiquitination
S88 Phosphorylation
S89 Phosphorylation
S90 Phosphorylation
S95 Phosphorylation
K96 Ubiquitination
K102 Ubiquitination
K107 Ubiquitination
S109 Phosphorylation
K121 Ubiquitination
K140 Ubiquitination
K232 Ubiquitination
K268 Ubiquitination
K332 Ubiquitination
K334 Ubiquitination
K367 Ubiquitination
K371 Ubiquitination
K409 Ubiquitination
Y451 Phosphorylation
T461 Phosphorylation
K479 Ubiquitination
Y488 Phosphorylation
K489 Ubiquitination
K500 Ubiquitination
K508 Ubiquitination
Y519 Phosphorylation
R523 Methylation
Y532 Phosphorylation
S533 Phosphorylation
T536 Phosphorylation
T538 Phosphorylation
K548 Ubiquitination
T553 Phosphorylation
K560 Ubiquitination
K572 Ubiquitination
S573 Phosphorylation P17612 (PRKACA)
Y580 Phosphorylation
T581 Phosphorylation
K583 Ubiquitination
K589 Ubiquitination
S598 Phosphorylation

研究背景

功能:

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.

细胞定位:

Cytoplasm. Nucleus.
Note: Nuclear in interphase, nuclear during mitosis.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Isoform Delta-2 is widely expressed. Isoform Delta-1 is highly expressed in brain.

亚基结构:

PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with SGO1. Interacts with ADCY8.

蛋白家族:

Belongs to the phosphatase 2A regulatory subunit B56 family.

研究领域

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Genetic Information Processing > Translation > mRNA surveillance pathway.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Nervous system > Dopaminergic synapse.

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