SLCO1B1 Antibody - #DF4534

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产品: SLCO1B1 抗体
货号: DF4534
描述: Rabbit polyclonal antibody to SLCO1B1
应用: WB IF/ICC
反应: Human, Mouse
分子量: 76 KD; 76kD(Calculated).
蛋白号: Q9Y6L6
RRID: AB_2819223

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
克隆:
Polyclonal
特异性:
SLCO1B1 Antibody detects endogenous levels of total SLCO1B1.
RRID:
AB_2819223
引用格式: Affinity Biosciences Cat# DF4534, RRID:AB_2819223.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Liver-specific organic anion transporter 1; LST-1; LST1; OATP-2; OATP-C; OATP1B1; OATPC; SLC21A6; SLCO1B1; SO1B1_HUMAN; Sodium-independent organic anion-transporting polypeptide 2; Solute carrier family 21 member 6; Solute carrier organic anion transporter family member 1B1;

抗原和靶标

免疫原:
Uniprot:

Q9Y6L6(SO1B1_HUMAN) >>Visit HPA database.

基因/基因ID:
SLCO1B1(10599)
表达:
Q9Y6L6 SO1B1_HUMAN:

Highly expressed in liver, at the basolateral membranes of centrilobular hepatocytes. Not detected in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocyte.

序列:
MDQNQHLNKTAEAQPSENKKTRYCNGLKMFLAALSLSFIAKTLGAIIMKSSIIHIERRFEISSSLVGFIDGSFEIGNLLVIVFVSYFGSKLHRPKLIGIGCFIMGIGGVLTALPHFFMGYYRYSKETNINSSENSTSTLSTCLINQILSLNRASPEIVGKGCLKESGSYMWIYVFMGNMLRGIGETPIVPLGLSYIDDFAKEGHSSLYLGILNAIAMIGPIIGFTLGSLFSKMYVDIGYVDLSTIRITPTDSRWVGAWWLNFLVSGLFSIISSIPFFFLPQTPNKPQKERKASLSLHVLETNDEKDQTANLTNQGKNITKNVTGFFQSFKSILTNPLYVMFVLLTLLQVSSYIGAFTYVFKYVEQQYGQPSSKANILLGVITIPIFASGMFLGGYIIKKFKLNTVGIAKFSCFTAVMSLSFYLLYFFILCENKSVAGLTMTYDGNNPVTSHRDVPLSYCNSDCNCDESQWEPVCGNNGITYISPCLAGCKSSSGNKKPIVFYNCSCLEVTGLQNRNYSAHLGECPRDDACTRKFYFFVAIQVLNLFFSALGGTSHVMLIVKIVQPELKSLALGFHSMVIRALGGILAPIYFGALIDTTCIKWSTNNCGTRGSCRTYNSTSFSRVYLGLSSMLRVSSLVLYIILIYAMKKKYQEKDINASENGSVMDEANLESLNKNKHFVPSAGADSETHC

翻译修饰 - Q9Y6L6 作为底物

Site PTM Type Enzyme
N134 N-Glycosylation
S293 Phosphorylation
S295 Phosphorylation
T301 Phosphorylation
T312 Phosphorylation
T319 Phosphorylation
T323 Phosphorylation
S328 Phosphorylation
Y367 Phosphorylation
S420 Phosphorylation
Y422 Phosphorylation
Y425 Phosphorylation
N503 N-Glycosylation
N516 N-Glycosylation
Y625 Phosphorylation
S636 Phosphorylation
Y645 Phosphorylation
S659 Phosphorylation
S663 Phosphorylation
S672 Phosphorylation
S682 Phosphorylation
S687 Phosphorylation
T689 Phosphorylation

研究背景

功能:

Mediates the Na(+)-independent uptake of organic anions such as pravastatin, taurocholate, methotrexate, dehydroepiandrosterone sulfate, 17-beta-glucuronosyl estradiol, estrone sulfate, prostaglandin E2, thromboxane B2, leukotriene C3, leukotriene E4, thyroxine and triiodothyronine. Involved in the clearance of bile acids and organic anions from the liver.

细胞定位:

Basolateral cell membrane>Multi-pass membrane protein.
Note: Detected in basolateral membranes of hepatocytes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Highly expressed in liver, at the basolateral membranes of centrilobular hepatocytes. Not detected in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocyte.

蛋白家族:

Belongs to the organo anion transporter (TC 2.A.60) family.

文献引用

1). Mn (II) Complex of Lipophilic Group-Modified Ethylenediaminetetraacetic Acid (EDTA) as a New Hepatobiliary MRI Contrast Agent. JOURNAL OF MEDICINAL CHEMISTRY, 2021 (PubMed: 34152137) [IF=7.3]
2). Based on disulfidptosis-related glycolytic genes to construct a signature for predicting prognosis and immune infiltration analysis of hepatocellular carcinoma. Frontiers in Immunology, 2023 (PubMed: 37680641) [IF=7.3]

Application: IHC    Species: Human    Sample: liver tissues

Figure 10 (A) Expression of the seven genes in the risk signature in the high-risk and low-risk groups. (B) Correlation of the seven genes in the train group. (C) Correlation of the seven genes in the test group. (D) Correlation between the level of immune cell infiltration and the seven genes in the risk model. (E) Differential expression of SLCO1B1 in HCC tissues and normal tissues. The red part represents HCC patient samples, and the gray part represents normal patient tissue samples. (F) The result of qRT-PCR. (G) Normal and cancer images of SLCO1B1 expression in liver tissues (100× and 400×) detected by IHC staining. (H) IHC score for all samples. **** represents P
3). Berberine Promotes OATP1B1 Expression and Rosuvastatin Uptake by Inducing Nuclear Translocation of FXR and LXRα. Frontiers in Pharmacology, 2020 (PubMed: 32292349) [IF=5.6]

Application: WB    Species: human    Sample: HepG2 cells

FIGURE 1 | Effects of berberine on OATP1B1 expression in HepG2 cells. (C, D) OATP1B1 protein levels were determined by Western blotting in HepG2 cells after treatment with increasing concentrations of berberine (5, 10, 25, and 50mM) for 24 h or treatment with 25 mM berberine at a series of time points (6, 12, 24, and 48 h). Data are represent as mean ± SD from triplicate independent experiments after being normalized to GAPDH, and 0.1% dimethylsulfoxide (DMSO) were used as the negative control. ***P < 0.001 compared to the control.
4). Pregnane X receptor promotes liver enlargement in mice through the spatial induction of hepatocyte hypertrophy and proliferation. Chemico-Biological Interactions, 2022 (PubMed: 36030841) [IF=5.1]
5). An integrated strategy combining network toxicology and feature-based molecular networking for exploring hepatotoxic constituents and mechanism of Epimedii Folium-induced hepatotoxicity in vitro. Food and Chemical Toxicology, 2023 (PubMed: 37080529) [IF=4.3]
6). Dexamethasone-Induced Liver Enlargement Is Related to PXR/YAP Activation and Lipid Accumulation but Not Hepatocyte Proliferation. Drug Metabolism and Disposition, 2020 (PubMed: 32561593) [IF=3.9]

Application: WB    Species: Mouse    Sample:

Fig. 2. Dex activates mPXR and hPXR. (A and B) Western blot and quantification of mPXR downstream proteins from vehicle- or Dex-treated mice livers. Data are expressed as mean ± S.D. (n = 3). *P < 0.05; **P < 0.01 compared with the vehicle group. (C) Dual-luciferase reporter gene assay was used to determine the effect of RIF or Dex on hPXR activation in HEK293T cells. Data are expressed as mean ± S.D. (n = 5). **P < 0.01; ***P < 0.001; ****P < 0.0001 compared with the control group.

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