产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4604, RRID:AB_2836968.
展开/折叠
Atp6b1; ATP6V1B1; ATPase, H+ transporting, lysosomal (vacuolar proton pump), beta 56/58 kDa, isoform 1; ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B, isoform 1; ATPase, H+ transporting, V1 subunit B, isoform 1; Endomembrane proton pump 58 kDa subunit; H(+) transporting two sector ATPase 58kD subunit; H+ ATPase beta 1 subunit; kidney isoform; Lysosomal 56/58kDa; MGC32642; RTA1B; V ATPase B1 subunit; V type proton ATPase subunit B kidney isoform; V type proton ATPase subunit B, kidney isoform; V-ATPase subunit B 1; V-type proton ATPase subunit B; Vacuolar proton pump 3; Vacuolar proton pump subunit 3; Vacuolar proton pump subunit B 1; VATB; VATB1_HUMAN; VMA2; Vpp 3; Vpp3;
抗原和靶标
- P15313 VATB1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAMEIDSRPGGLPGSSCNLGAAREHMQAVTRNYITHPRVTYRTVCSVNGPLVVLDRVKFAQYAEIVHFTLPDGTQRSGQVLEVAGTKAIVQVFEGTSGIDARKTTCEFTGDILRTPVSEDMLGRVFNGSGKPIDKGPVVMAEDFLDINGQPINPHSRIYPEEMIQTGISPIDVMNSIARGQKIPIFSAAGLPHNEIAAQICRQAGLVKKSKAVLDYHDDNFAIVFAAMGVNMETARFFKSDFEQNGTMGNVCLFLNLANDPTIERIITPRLALTTAEFLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGRVEGRGGSITQIPILTMPNDDITHPIPDLTGFITEGQIYVDRQLHNRQIYPPINVLPSLSRLMKSAIGEGMTRKDHGDVSNQLYACYAIGKDVQAMKAVVGEEALTSEDLLYLEFLQKFEKNFINQGPYENRSVFESLDLGWKLLRIFPKEMLKRIPQAVIDEFYSREGALQDLAPDTAL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P15313 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S15 | Phosphorylation | Uniprot | |
| S118 | Phosphorylation | Uniprot | |
| K182 | Ubiquitination | Uniprot | |
| Y322 | Phosphorylation | Uniprot | |
| S391 | Phosphorylation | Uniprot | |
| S393 | Phosphorylation | Uniprot | |
| K397 | Ubiquitination | Uniprot | |
| S466 | Phosphorylation | Uniprot | |
| Y498 | Phosphorylation | Uniprot |
研究背景
Non-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.
Endomembrane system>Peripheral membrane protein.
Note: Endomembrane.
Expressed in the cochlea and endolymphatic sac.
V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (main components: subunits A, B, C, D, E, and F) attached to an integral membrane V0 proton pore complex (main component: the proteolipid protein). Forms a complex with SLC9A3R1 and SCL4A7.
The PDZ-binding motif mediates interactions with SLC9A3R1 and SCL4A7.
Belongs to the ATPase alpha/beta chains family.
研究领域
· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)
· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Vibrio cholerae infection.
· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.
· Human Diseases > Immune diseases > Rheumatoid arthritis.
· Metabolism > Energy metabolism > Oxidative phosphorylation.
· Metabolism > Global and overview maps > Metabolic pathways.
· Organismal Systems > Nervous system > Synaptic vesicle cycle.
· Organismal Systems > Excretory system > Collecting duct acid secretion.
限制条款
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