产品: Glucose-6-phosphate isomerase 抗体
货号: BF0106
描述: Mouse monoclonal antibody to Glucose-6-phosphate isomerase
应用: WB IHC IF/ICC ELISA
反应: Human
分子量: 63kDa; 63kD(Calculated).
蛋白号: P06744
RRID: AB_2833633

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 50ul RMB¥ 1800 现货
 100ul RMB¥ 2800 现货

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产品描述

来源:
Mouse
应用:
ELISA 1:10000, WB 1:500-1:2000, IHC 1:200-1:1000, IF/ICC 1:200-1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
克隆:
Monoclonal [AFB1609]
特异性:
Glucose-6-phosphate isomerase antibody detects endogenous levels of total Glucose-6-phosphate isomerase.
RRID:
AB_2833633
引用格式: Affinity Biosciences Cat# BF0106, RRID:AB_2833633.
偶联:
Unconjugated.
纯化:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AMF; Aurocrine motility factor; Autocrine motility factor; DKFZp686C13233; EC 5.3.1.9; G6PI_HUMAN; Glucose phosphate isomerase; Glucose-6-phosphate isomerase; GNPI; GPI; Gpi1; Hexose monophosphate isomerase; Hexosephosphate isomerase; Neuroleukin; NLK; Oxoisomerase; PGI; PHI; Phosphoglucose isomerase; Phosphohexomutase; Phosphohexose isomerase; Phosphosaccharomutase; SA 36; SA-36; SA36; Sperm antigen 36;

抗原和靶标

免疫原:

Purified recombinant fragment of human Glucose-6-phosphate isomerase expressed in E. Coli.

Uniprot:
基因/基因ID:
描述:
Glucose-6-phosphate isomerase, or phosphoglucose isomerase, also known as GPI. It belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways and it is an enzyme that catalyzes the conversion of glucose-6-phosphate into fructose 6-phosphate in the second step of glycolysis. The protein functions in different capacities inside and outside the cell.
序列:
MAALTRDPQFQKLQQWYREHRSELNLRRLFDANKDRFNHFSLTLNTNHGHILVDYSKNLVTEDVMRMLVDLAKSRGVEAARERMFNGEKINYTEGRAVLHVALRNRSNTPILVDGKDVMPEVNKVLDKMKSFCQRVRSGDWKGYTGKTITDVINIGIGGSDLGPLMVTEALKPYSSGGPRVWYVSNIDGTHIAKTLAQLNPESSLFIIASKTFTTQETITNAETAKEWFLQAAKDPSAVAKHFVALSTNTTKVKEFGIDPQNMFEFWDWVGGRYSLWSAIGLSIALHVGFDNFEQLLSGAHWMDQHFRTTPLEKNAPVLLALLGIWYINCFGCETHAMLPYDQYLHRFAAYFQQGDMESNGKYITKSGTRVDHQTGPIVWGEPGTNGQHAFYQLIHQGTKMIPCDFLIPVQTQHPIRKGLHHKILLANFLAQTEALMRGKSTEEARKELQAAGKSPEDLERLLPHKVFEGNRPTNSIVFTKLTPFMLGALVAMYEHKIFVQGIIWDINSFDQWGVELGKQLAKKIEPELDGSAQVTSHDASTNGLINFIKQQREARVQ

翻译修饰 - P06744 作为底物

Site PTM Type Enzyme
A2 Acetylation
K12 Acetylation
K12 Methylation
K12 Ubiquitination
Y17 Phosphorylation
S22 Phosphorylation
K34 Acetylation
K34 Ubiquitination
Y55 Phosphorylation
S56 Phosphorylation
T61 Phosphorylation
R66 Methylation
K73 Ubiquitination
S74 Phosphorylation
K89 Acetylation
K89 Ubiquitination
Y92 Phosphorylation
T93 Phosphorylation
R106 Methylation
S107 Phosphorylation
T109 Phosphorylation
K116 Acetylation
K116 Ubiquitination
K124 Acetylation
K124 Ubiquitination
K130 Ubiquitination
S131 Phosphorylation
S138 Phosphorylation
K142 Acetylation
K142 Ubiquitination
K147 Ubiquitination
S160 Phosphorylation
T168 Phosphorylation
K172 Ubiquitination
Y174 Phosphorylation
S176 Phosphorylation
Y183 Phosphorylation
S185 Phosphorylation P68400 (CSNK2A1)
K194 Ubiquitination
T195 Phosphorylation
S210 Phosphorylation
K211 Acetylation
K211 Ubiquitination
T214 Phosphorylation
T215 Phosphorylation
K226 Ubiquitination
K234 Acetylation
K234 Ubiquitination
S237 Phosphorylation
K241 Ubiquitination
S247 Phosphorylation
T248 Phosphorylation
T250 Phosphorylation
T251 Phosphorylation
K252 Acetylation
K252 Ubiquitination
S359 Phosphorylation
K362 Acetylation
K362 Ubiquitination
K366 Ubiquitination
C404 S-Nitrosylation
T412 Phosphorylation
K423 Ubiquitination
R438 Methylation
S441 Phosphorylation
K447 Ubiquitination
K454 Acetylation
K454 Ubiquitination
S455 Phosphorylation
K466 Acetylation
K466 Sumoylation
K466 Ubiquitination
K524 Ubiquitination
S532 Phosphorylation

研究背景

功能:

In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis. Besides it's role as a glycolytic enzyme, also acts as a secreted cytokine: acts as an angiogenic factor (AMF) that stimulates endothelial cell motility. Acts as a neurotrophic factor, neuroleukin, for spinal and sensory neurons. It is secreted by lectin-stimulated T-cells and induces immunoglobulin secretion.

翻译修饰:

Phosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway.

ISGylated.

细胞定位:

Cytoplasm. Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Homodimer; in the catalytically active form. Monomer in the secreted form.

蛋白家族:

Belongs to the GPI family.

研究领域

· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.

· Metabolism > Carbohydrate metabolism > Pentose phosphate pathway.

· Metabolism > Carbohydrate metabolism > Starch and sucrose metabolism.

· Metabolism > Carbohydrate metabolism > Amino sugar and nucleotide sugar metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

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