产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4611, RRID:AB_2836975.
展开/折叠
N ethylmaleimide sensitive factor; N ethylmaleimide sensitive factor like protein; N ethylmaleimide sensitive fusion protein; N-ethylmaleimide-sensitive fusion protein; NEM sensitive fusion protein; NEM-sensitive fusion protein; NSF; NSF_HUMAN; SKD 2; SKD2; Vesicle fusing ATPase; Vesicle-fusing ATPase; Vesicular fusion protein NSF; Vesicular-fusion protein NSF;
抗原和靶标
- P46459 NSF_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGRSMQAARCPTDELSLTNCAVVNEKDFQSGQHVIVRTSPNHRYTFTLKTHPSVVPGSIAFSLPQRKWAGLSIGQEIEVSLYTFDKAKQCIGTMTIEIDFLQKKSIDSNPYDTDKMAAEFIQQFNNQAFSVGQQLVFSFNEKLFGLLVKDIEAMDPSILKGEPATGKRQKIEVGLVVGNSQVAFEKAENSSLNLIGKAKTKENRQSIINPDWNFEKMGIGGLDKEFSDIFRRAFASRVFPPEIVEQMGCKHVKGILLYGPPGCGKTLLARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQRRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDEKGRLQILHIHTARMRGHQLLSADVDIKELAVETKNFSGAELEGLVRAAQSTAMNRHIKASTKVEVDMEKAESLQVTRGDFLASLENDIKPAFGTNQEDYASYIMNGIIKWGDPVTRVLDDGELLVQQTKNSDRTPLVSVLLEGPPHSGKTALAAKIAEESNFPFIKICSPDKMIGFSETAKCQAMKKIFDDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLLKKAPPQGRKLLIIGTTSRKDVLQEMEMLNAFSTTIHVPNIATGEQLLEALELLGNFKDKERTTIAQQVKGKKVWIGIKKLLMLIEMSLQMDPEYRVRKFLALLREEGASPLDFD
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P46459 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| C21 | S-Nitrosylation | Uniprot | |
| Y45 | Phosphorylation | Uniprot | |
| Y83 | Phosphorylation | P16591 (FER) , P07332 (FES) | Uniprot |
| C91 | S-Nitrosylation | Uniprot | |
| S106 | Phosphorylation | Uniprot | |
| S109 | Phosphorylation | Uniprot | |
| Y112 | Phosphorylation | Uniprot | |
| T114 | Phosphorylation | Uniprot | |
| S158 | Phosphorylation | Uniprot | |
| K161 | Ubiquitination | Uniprot | |
| T166 | Phosphorylation | Uniprot | |
| K198 | Ubiquitination | Uniprot | |
| S207 | Phosphorylation | Uniprot | |
| K217 | Ubiquitination | Uniprot | |
| S237 | Phosphorylation | Uniprot | |
| C250 | S-Nitrosylation | Uniprot | |
| K254 | Ubiquitination | Uniprot | |
| C264 | S-Nitrosylation | Uniprot | |
| K266 | Ubiquitination | Uniprot | |
| K275 | Ubiquitination | Uniprot | |
| K283 | Ubiquitination | Uniprot | |
| K293 | Ubiquitination | Uniprot | |
| S298 | Phosphorylation | Uniprot | |
| R337 | Methylation | Uniprot | |
| S339 | Phosphorylation | Uniprot | |
| T349 | Phosphorylation | Uniprot | |
| S356 | Phosphorylation | Uniprot | |
| K401 | Ubiquitination | Uniprot | |
| K434 | Ubiquitination | Uniprot | |
| S437 | Phosphorylation | Uniprot | |
| S460 | Phosphorylation | Q02156 (PRKCE) | Uniprot |
| T461 | Phosphorylation | Q02156 (PRKCE) | Uniprot |
| K462 | Ubiquitination | Uniprot | |
| K469 | Ubiquitination | Uniprot | |
| S472 | Phosphorylation | Uniprot | |
| K529 | Ubiquitination | Uniprot | |
| T534 | Phosphorylation | Uniprot | |
| S547 | Phosphorylation | Uniprot | |
| K555 | Ubiquitination | Uniprot | |
| K566 | Ubiquitination | Uniprot | |
| S569 | Phosphorylation | Uniprot | |
| K572 | Ubiquitination | Uniprot | |
| K581 | Ubiquitination | Uniprot | |
| K587 | Ubiquitination | Uniprot | |
| Y611 | Phosphorylation | Uniprot | |
| T645 | Phosphorylation | Q5S007 (LRRK2) | Uniprot |
| T646 | Phosphorylation | Uniprot | |
| S647 | Phosphorylation | Uniprot | |
| T693 | Phosphorylation | Uniprot | |
| K699 | Ubiquitination | Uniprot | |
| S717 | Phosphorylation | Uniprot | |
| Y724 | Phosphorylation | Uniprot | |
| S739 | Phosphorylation | Uniprot |
研究背景
Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling (By similarity).
Phosphorylation at Ser-569 interferes with homohexamerization.
Cytoplasm.
Homohexamer. Interacts with GABARAP and GABARAPL2. Interacts with GRIA2. Interacts with PLK2, leading to disrupt the interaction with GRIA2. Interacts with MUSK; may regulate MUSK endocytosis and activity (By similarity). Interacts with CDK16 (By similarity).
Belongs to the AAA ATPase family.
研究领域
· Organismal Systems > Nervous system > Synaptic vesicle cycle.
· Organismal Systems > Nervous system > GABAergic synapse.
· Organismal Systems > Excretory system > Vasopressin-regulated water reabsorption.
限制条款
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