ABCG2 Antibody - #AF5177

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产品: ABCG2 抗体
货号: AF5177
描述: Rabbit polyclonal antibody to ABCG2
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Rabbit
分子量: 72 kDa; 72kD(Calculated).
蛋白号: Q9UNQ0
RRID: AB_2837663

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产品描述

来源:
Rabbit
应用:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Rabbit(89%)
克隆:
Polyclonal
特异性:
ABCG2 Antibody detects endogenous levels of total ABCG2.
RRID:
AB_2837663
引用格式: Affinity Biosciences Cat# AF5177, RRID:AB_2837663.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ABC transporter; ABC15; ABCG 2; ABCG2; ABCG2_HUMAN; ABCP; ATP binding cassette sub family G (WHITE) member 2; ATP binding cassette transporter G2; ATP-binding cassette sub-family G member 2; BCRP; BCRP1; BMDP; Breast cancer resistance protein; CD338; CDw338; CDw338 antigen; EST157481; GOUT1; MGC102821; Mitoxantrone resistance associated protein; Mitoxantrone resistance-associated protein; MRX; Multi drug resistance efflux transport ATP binding cassette sub family G (WHITE) member 2; MXR; MXR1; Placenta specific ATP binding cassette transporter; Placenta specific MDR protein; Placenta-specific ATP-binding cassette transporter; UAQTL1;

抗原和靶标

免疫原:
Uniprot:

Q9UNQ0(ABCG2_HUMAN) >>Visit HPA database.

基因/基因ID:
ABCG2(9429)
表达:
Q9UNQ0 ABCG2_HUMAN:

Highly expressed in placenta (PubMed:9850061). Low expression in small intestine, liver and colon (PubMed:9861027). Expressed in brain (at protein level) (PubMed:12958161).

描述:
High-capacity urate exporter functioning in both renal and extrarenal urate excretion. Plays a role in porphyrin homeostasis as it is able to mediates the export of protoporhyrin IX (PPIX) both from mitochondria to cytosol and from cytosol to extracellular space, and cellular export of hemin, and heme. Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain.
序列:
MSSSNVEVFIPVSQGNTNGFPATASNDLKAFTEGAVLSFHNICYRVKLKSGFLPCRKPVEKEILSNINGIMKPGLNAILGPTGGGKSSLLDVLAARKDPSGLSGDVLINGAPRPANFKCNSGYVVQDDVVMGTLTVRENLQFSAALRLATTMTNHEKNERINRVIQELGLDKVADSKVGTQFIRGVSGGERKRTSIGMELITDPSILFLDEPTTGLDSSTANAVLLLLKRMSKQGRTIIFSIHQPRYSIFKLFDSLTLLASGRLMFHGPAQEALGYFESAGYHCEAYNNPADFFLDIINGDSTAVALNREEDFKATEIIEPSKQDKPLIEKLAEIYVNSSFYKETKAELHQLSGGEKKKKITVFKEISYTTSFCHQLRWVSKRSFKNLLGNPQASIAQIIVTVVLGLVIGAIYFGLKNDSTGIQNRAGVLFFLTTNQCFSSVSAVELFVVEKKLFIHEYISGYYRVSSYFLGKLLSDLLPMRMLPSIIFTCIVYFMLGLKPKADAFFVMMFTLMMVAYSASSMALAIAAGQSVVSVATLLMTICFVFMMIFSGLLVNLTTIASWLSWLQYFSIPRYGFTALQHNEFLGQNFCPGLNATGNNPCNYATCTGEEYLVKQGIDLSPWGLWKNHVALACMIVIFLTIAYLKLLFLKKYS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Rabbit
89
Bovine
78
Sheep
78
Pig
75
Horse
67
Xenopus
56
Dog
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9UNQ0 作为底物

Site PTM Type Enzyme
S50 Phosphorylation
S100 Phosphorylation
S103 Phosphorylation
S143 Phosphorylation
K172 Ubiquitination
S187 Phosphorylation
T194 Phosphorylation
S195 Phosphorylation
K323 Ubiquitination
S353 Phosphorylation
K357 Ubiquitination
T362 Phosphorylation P11309 (PIM1)
Y464 Phosphorylation
N596 N-Glycosylation

研究背景

功能:

Broad substrate specificity ATP-dependent transporter of the ATP-binding cassette (ABC) family that actively extrudes a wide variety of physiological compounds, dietary toxins and xenobiotics from cells. Involved in porphyrin homeostasis, mediating the export of protoporphyrin IX (PPIX) from both mitochondria to cytosol and cytosol to extracellular space, it also functions in the cellular export of heme. Also mediates the efflux of sphingosine-1-P from cells. Acts as a urate exporter functioning in both renal and extrarenal urate excretion. In kidney, it also functions as a physiological exporter of the uremic toxin indoxyl sulfate (By similarity). Also involved in the excretion of steroids like estrone 3-sulfate/E1S, 3beta-sulfooxy-androst-5-en-17-one/DHEAS, and other sulfate conjugates. Mediates the secretion of the riboflavin and biotin vitamins into milk (By similarity). Extrudes pheophorbide a, a phototoxic porphyrin catabolite of chlorophyll, reducing its bioavailability (By similarity). Plays an important role in the exclusion of xenobiotics from the brain (Probable). It confers to cells a resistance to multiple drugs and other xenobiotics including mitoxantrone, pheophorbide, camptothecin, methotrexate, azidothymidine, and the anthracyclines daunorubicin and doxorubicin, through the control of their efflux. In placenta, it limits the penetration of drugs from the maternal plasma into the fetus (By similarity). May play a role in early stem cell self-renewal by blocking differentiation (By similarity).

翻译修饰:

N-glycosylated. Glycosylation-deficient ABCG2 is normally expressed and functional.

Phosphorylated. Phosphorylation at Thr-362 by PIM1 is induced by drugs like mitoxantrone and is associated with cells increased drug resistance. It regulates the localization to the plasma membrane, the homooligomerization and therefore, the activity of the transporter.

细胞定位:

Cell membrane>Multi-pass membrane protein. Apical cell membrane>Multi-pass membrane protein. Mitochondrion membrane>Multi-pass membrane protein.
Note: Enriched in membrane lipid rafts.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Highly expressed in placenta. Low expression in small intestine, liver and colon. Expressed in brain (at protein level).

亚基结构:

Homodimer; disulfide-linked. The minimal functional unit is a homodimer, but the major oligomeric form in plasma membrane is a homotetramer with possibility of higher order oligomerization up to homododecamers.

蛋白家族:

The extracellular loop 3 (ECL3) is involved in binding porphyrins and transfer them to other carriers, probably albumin.

Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.

研究领域

· Environmental Information Processing > Membrane transport > ABC transporters.

· Human Diseases > Drug resistance: Antineoplastic > Antifolate resistance.

文献引用

1). Caffeic acid phenethyl ester alleviated hypouricemia in hyperuricemic mice through inhibiting XOD and up-regulating OAT3. PHYTOMEDICINE (PubMed: 35714456) [IF=7.9]
2). Whey Protein Peptide Pro-Glu-Trp Ameliorates Hyperuricemia by Enhancing Intestinal Uric Acid Excretion, Modulating the Gut Microbiota, and Protecting the Intestinal Barrier in Rats. Journal of agricultural and food chemistry (PubMed: 38240209) [IF=6.1]
3). MiR-21 modulates the polarization of M2 macrophages and increases the effects of M2 macrophages on promoting the chemoresistance of ovarian cancer. LIFE SCIENCES (PubMed: 31837336) [IF=6.1]

Application: WB    Species: human    Sample: ovarian cancer cells

Figure 2. |Characterization of M0, M1 and M2 macrophages. M2 macrophages could promote the chemoresistance of ovarian cancer cells.(F) Western blotting was used to test the expression levels of cleaved caspase 3, ABCG2 and MDR1 in the ovarian cancer cells which co-cultured with M0 and M2 macrophages. U6 and GAPDH were used as the control. The columns showed as the mean ± SE of triplicate samples. *P < 0.05; **P < 0.01; ***P < 0.001.
4). RPF2 mediates the CARM1‑MYCN axis to promote chemotherapy resistance in colorectal cancer cells. Oncology reports (PubMed: 37997821) [IF=4.2]
5). Hypouricaemic and nephroprotective effects of Poria cocos in hyperuricemic mice by up-regulating ATP-binding cassette super-family G member 2. PHARMACEUTICAL BIOLOGY (PubMed: 33651969) [IF=3.8]

Application: WB    Species: Mice    Sample: kidney tissues

Figure 4. Effects of PCE and PCW on renal ABCG2, OAT3, OAT1 and OCT2 protein expression detected by Western blot: immunoreactive bands (a) and densitometries (b,c,d and –e, expressed as mean ± SD; n n ¼ 3).  p < 0.05,  p < 0.01 versus the normal control; #p < 0.05, ##p < 0.01 versus the hyperuricemic control;  p < 0.01 versus the allopurinol control.
6). NRBP1 modulates uric acid transporter ABCG2 expression by activating the Wnt/β-catenin pathway in HK-2 cells. Nefrología (English Edition) (PubMed: 36437206)

Application: WB    Species: Human    Sample: HK-2 cells

Fig. 2. Effect of NRBP1 overexpression and knockdown on the secretion and reabsorption of uric acid. A, Effect of NRBP1 overexpression and knockdown on the mRNA expression of NRBP1, ABCG2, OAT1, GLUT9 and URAT1. B, Effect of NRBP1 overexpression and knockdown on the protein expression of NRBP1, ABCG2, OAT1, GLUT9 and URAT1.
7). Curcuma zedoaria petroleum ether extract reverses the resistance of triple-negative breast cancer to docetaxel via pregnane X receptor. Annals of Translational Medicine (PubMed: 34733941)

Application: WB    Species: Mice    Sample: MDA-MB-231/docetaxel cells

Figure 3 Effect of PECZ on the protein expression of drug resistance genes in MDA-MB-231/docetaxel cells. Representative band of each protein (A). Relative protein expression of PXR (B), MDR1 (C), BCRP (D), and CYP3A4 (E). (x¯±s, n=3), *, P<0.05; **, P<0.01 vs. control group. PECZ, petroleum ether extracts of Curcuma zedoaria; PXR, pregnane X receptor; MDR1, multidrug resistance 1; BCRP, breast cancer resistance protein; CYP3A4, cytochrome P-450.

Application: IHC    Species: Mice    Sample: tumor tissues

Figure 7 PECZ and docetaxel decreased the expression of drug resistance genes. Representative images of immunohistochemical showing PXR, MDR1, CYP3A4, and BCRP in tumor tissues in the different groups. Original magnification ×200, ×400; (x¯±s, n=3). PECZ, petroleum ether extracts of Curcuma zedoaria; PXR, pregnane X receptor; MDR1, multidrug resistance 1; BCRP, breast cancer resistance protein; CYP3A4, cytochrome P-450.
8). IL-17A exacerbates cisplatin-based resistance of OVCA via upregulating the expression of ABCG2 and MDR1 through Gli1-mediated Hh signaling. Oncotarget

Application: WB    Species: Human    Sample: A2780

Figure 4: Gli1-mediated Hh signal pathway is involved in the enhancing effect of rhIL-17A on DDP-based resistance of OVCA cells. A. Up-regulating effects of rhIL-17A on the levels of ABCG2, MDR1 and Gli1 in A2780 and OVCAR3 cells could be abolished by neutralizing IL-17RA mAb or IL-17RA knockdown. OVCA cells were pretreated with 3μg/ml neutralizing IL-17RA mAb for 1h (a) or transient silenced by siRNA targeting IL-17RA for 48h (b), and then treated with 1ng/ml rhIL-17A for 24h. B. Up-regulating effects of rhIL-17A on the expression of ABCG2, MDR1 and Gli1 in A2780 and OVCAR3 cells could be blocked by Gant61. OVCA cells were pretreated with 25μM Gant 61 for 1h and then treated with 1ng/ml rhIL-17A for 24h. Protein lysates from OVCA cells were prepared and analyzed by western blotting. Representative result from three independent experiments was showed. C. Gant61 could partially reverse the increase-effect of rhIL-17A on OVCA cell viability. A2780 and OVCAR3 cells were pretreated with 25μM Gant 61 for 1h and then treated with 1ng/ml rhIL-17A and/or DDP (10μM for A2780 cells, 100μM for OVCAR3 cells) for 24h. Cell viability was detected by MTT assay. Data represent means±SD from three independent experiments.

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