Patched/PTCH1 Antibody - #AF5202

>>买3送1 进行中>>
(7)   (10)  
产品: Patched/PTCH1 抗体
货号: AF5202
描述: Rabbit polyclonal antibody to Patched/PTCH1
应用: WB IHC IF/ICC IHC-P
反应: Human, Mouse, Rat
分子量: 160kDa; 161kD(Calculated).
蛋白号: Q13635
RRID: AB_2837688

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC-p 1:50-1:200, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
克隆:
Polyclonal
特异性:
Patched/PTCH1 Antibody detects endogenous levels of total Patched.
RRID:
AB_2837688
引用格式: Affinity Biosciences Cat# AF5202, RRID:AB_2837688.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

A230106A15Rik; BCNS; FLJ26746; FLJ42602; Holoprosencephaly 7; HPE7; mes; NBCCS; OTTHUMP00000021709; OTTHUMP00000021710; Patched; Patched (Drosophila) homolog; Patched 1; Patched homolog (Drosophila); Patched homolog 1 (Drosophila); Patched homolog 1; Patched protein homolog 1; Protein patched homolog 1; PTC; PTC1; PTC1_HUMAN; PTCH; PTCH protein +12b; PTCH protein +4'; PTCH protein -10; PTCH protein; ptch1; PTCH1 protein; PTCH11;

抗原和靶标

免疫原:
Uniprot:

Q13635(PTC1_HUMAN) >>Visit HPA database.

基因/基因ID:
PTCH1(5727)
表达:
Q13635 PTC1_HUMAN:

In the adult, expressed in brain, lung, liver, heart, placenta, skeletal muscle, pancreas and kidney. Expressed in tumor cells but not in normal skin.

描述:
Acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis
序列:
MASAGNAAEPQDRGGGGSGCIGAPGRPAGGGRRRRTGGLRRAAAPDRDYLHRPSYCDAAFALEQISKGKATGRKAPLWLRAKFQRLLFKLGCYIQKNCGKFLVVGLLIFGAFAVGLKAANLETNVEELWVEVGGRVSRELNYTRQKIGEEAMFNPQLMIQTPKEEGANVLTTEALLQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITETGYMDQIIEYLYPCLIITPLDCFWEGAKLQSGTAYLLGKPPLRWTNFDPLEFLEELKKINYQVDSWEEMLNKAEVGHGYMDRPCLNPADPDCPATAPNKNSTKPLDMALVLNGGCHGLSRKYMHWQEELIVGGTVKNSTGKLVSAHALQTMFQLMTPKQMYEHFKGYEYVSHINWNEDKAAAILEAWQRTYVEVVHQSVAQNSTQKVLSFTTTTLDDILKSFSDVSVIRVASGYLLMLAYACLTMLRWDCSKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVFLLAHAFSETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVSRVIQVEPQAYTDTHDNTRYSPPPPYSSHSFAHETQITMQSTVQLRTEYDPHTHVYYTTAEPRSEISVQPVTVTQDTLSCQSPESTSSTRDLLSQFSDSSLHCLEPPCTKWTLSSFAEKHYAPFLLKPKAKVVVIFLFLGLLGVSLYGTTRVRDGLDLTDIVPRETREYDFIAAQFKYFSFYNMYIVTQKADYPNIQHLLYDLHRSFSNVKYVMLEENKQLPKMWLHYFRDWLQGLQDAFDSDWETGKIMPNNYKNGSDDGVLAYKLLVQTGSRDKPIDISQLTKQRLVDADGIINPSAFYIYLTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRDTSDFVEAIEKVRTICSNYTSLGLSSYPNGYPFLFWEQYIGLRHWLLLFISVVLACTFLVCAVFLLNPWTAGIIVMVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHVALAFLTAIGDKNRRAVLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTILGVLNGLVLLPVLLSFFGPYPEVSPANGLNRLPTPSPEPPPSVVRFAMPPGHTHSGSDSSDSEYSSQTTVSGLSEELRHYEAQQGAGGPAHQVIVEATENPVFAHSTVVHPESRHHPPSNPRQQPHLDSGSLPPGRQGQQPRRDPPREGLWPPPYRPRRDAFEISTEGHSGPSNRARWGPRGARSHNPRNPASTAMGSSVPGYCQPITTVTASASVTVAVHPPPVPGPGRNPRGGLCPGYPETDHGLFEDPHVPFHVRCERRDSKVEVIELQDVECEERPRGSSSN

翻译修饰 - Q13635 作为底物

Site PTM Type Enzyme
Ubiquitination
K163 Ubiquitination
T368 Phosphorylation
S435 Phosphorylation
S463 Phosphorylation
K993 Ubiquitination
T1195 Phosphorylation
S1197 Phosphorylation
S1331 Phosphorylation
R1394 Methylation
S1425 Phosphorylation

研究背景

功能:

Acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis.

翻译修饰:

Glycosylation is necessary for SHH binding.

In the absence of Hh ligands, ubiquitination by ITCH at Lys-1426 promotes endocytosis and both proteasomal and lysosomal degradation.

细胞定位:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

In the adult, expressed in brain, lung, liver, heart, placenta, skeletal muscle, pancreas and kidney. Expressed in tumor cells but not in normal skin.

亚基结构:

Interacts with SNX17. Interacts with IHH. Interacts with G-protein coupled receptor GPR37L1 (By similarity).

蛋白家族:

Belongs to the patched family.

研究领域

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hedgehog signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Basal cell carcinoma.   (View pathway)

· Organismal Systems > Development > Axon guidance.   (View pathway)

文献引用

1). Silica Perturbs Primary Cilia and Causes Myofibroblast Differentiation during Silicosis by Reduction of the KIF3A-Repressor GLI3 Complex. Theranostics (PubMed: 32042332) [IF=12.4]

Application: WB    Species: human    Sample: MRC-5 fibroblasts

Figure 6. Detection of SHH signalling proteins in MRC-5 fibroblasts in response to SiO2 stimulation and KIF3A knockdown. (A, B) Western blot and densitometric analyses of SHH, PTC1, SMO, and GLI1 proteins in MRC-5 fibroblasts treated with SiO2 for 12 h together with KIF3A knockdown until 36 h. The relative density was normalised to α-Tub. Bar graphs are the means±SD of three separate experiments. *P<0.05; **P<0.01. Data are the mean±SD. Statistical analysis was performed using one-way ANOVA and SPSS 20.0. (C) Schematic of the primary cilia influence on SHH signalling. Bidirectional transcriptional factors regulate the transcription of target genes.

Application: WB    Species: Human    Sample: MRC-5 fibroblasts

Figure 6 Detection of SHH signalling proteins in MRC-5 fibroblasts in response to SiO2 stimulation and KIF3A knockdown. (A, B) Western blot and densitometric analyses of SHH, PTC1, SMO, and GLI1 proteins in MRC-5 fibroblasts treated with SiO2 for 12 h together with KIF3A knockdown until 36 h. The relative density was normalised to α-Tub. Bar graphs are the means±SD of three separate experiments. *P<0.05; **P<0.01. Data are the mean±SD. Statistical analysis was performed using one-way ANOVA and SPSS 20.0. (C) Schematic of the primary cilia influence on SHH signalling. Bidirectional transcriptional factors regulate the transcription of target genes.
2). Hedgehog pathway inhibition causes primary follicle atresia and decreases female germline stem cell proliferation capacity or stemness. Stem Cell Research & Therapy (PubMed: 31277696) [IF=7.5]

Application: WB    Species: mouse    Sample: ovarian

Fig. 1 | Hh signaling pathway activity decreases with ovarian aging. a mRNA expression levels of Gli1, Ptch1, and Cyclin D1 in three mice of reproductive age. b, c Protein expression levels.

Application: IHC    Species: mouse    Sample: ovarian

Fig. 1 | Hh signaling pathway activity decreases with ovarian aging. a mRNA expression levels of Gli1, Ptch1, and Cyclin D1 in three mice of reproductive age. b, c Protein expression levels.d IHC detection of key members of the Hh pathway, Gli1, Ptch1, and Cyclin D1, in the OSE. Bar is 10 μm. *p < 0.05 and **p < 0.01

Application: WB    Species: mouse    Sample: ovaries

Fig. |1 Hh signaling pathway activity decreases with ovarian aging. a mRNA expression levels of Gli1, Ptch1, and Cyclin D1 in three mice of reproductive age. b, c Protein expression levels.

Application: IHC    Species: mouse    Sample: ovaries

Fig. |1 Hh signaling pathway activity decreases with ovarian aging. a mRNA expression levels of Gli1, Ptch1, and Cyclin D1 in three mice of reproductive age. b, c Protein expression levels.d IHC detection of key members of the Hh pathway, Gli1, Ptch1, and Cyclin D1, in the OSE. Bar is 10 μm. *p < 0.05 and **p < 0.01
3). Effects of metformin on Sonic hedgehog subgroup medulloblastoma progression: In vitro and in vivo studies. Frontiers in Pharmacology (PubMed: 36278239) [IF=5.6]
4). Gankyrin activates the hedgehog signalling to drive metastasis in osteosarcoma. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (PubMed: 34089292) [IF=5.3]

Application: WB    Species: Human    Sample: U2OS cells

FIGURE 3 Gankyrin promotes invasion, migration and regulates the expression of some stemness factors by up‐regulating Gli1. A, Gankyrin knockdown in U2OS cells reduced the mRNA expression of Gli1 and Ptch1 but not of β‐catenin and AXIN2, as shown by qRT‐PCR analysis. ns, P ≥ .05; *P < .05. B, Overexpression of gankyrin in MG63 cells increased the mRNA expression of Gli1 and Ptch1 but not of β‐catenin and AXIN2. ns, P ≥ .05; **P < .01. C, Gankyrin depletion in U2OS cells diminished the protein levels of Gli1 and PTCH1, as detected by western blot analysis. D, Ectopic expression of gankyrin in MG63 cells induced the protein levels of Gli1 and PTCH1. E, The up‐regulation of stem cell markers, including CD133, OCT4, and Nanog, following gankyrin overexpression was attenuated by Gli1 shRNA in U2OS cells, as detected by Western blot analysis. F, The increase in cell migration with gankyrin overexpression was attenuated by Gli1 shRNA in U2OS cells, as shown by wound healing assays. *P < .05; **P < .01. G, The increase in cell migration with gankyrin overexpression was attenuated by Gli1 shRNA in U2OS cells, as shown by Transwell invasion assays. *P < .05
5). Curcumin mitigates the epithelial-to-mesenchymal transition in biliary epithelial cells through upregulating CD109 expression. DRUG DEVELOPMENT RESEARCH (PubMed: 31403228) [IF=3.8]

Application: WB    Species: human    Sample: HIBECs

FIGURE 5 |Curcumin suppresses the TGF-β1-activated Hedgehog signaling in HIBECs. (a) Quantitative analysis of Shh, Gli1, Smo, Ptch-1, and Sufu protein expression.
6). Targeting the RAS axis alleviates silicotic fibrosis and Ang II-induced myofibroblast differentiation via inhibition of the hedgehog signaling pathway. Toxicology Letters (PubMed: 31181250) [IF=3.5]
7). Metformin exerts anti-tumor effects via Sonic hedgehog signaling pathway by targeting AMPK in HepG2 cells. Biochemistry and Cell Biology (PubMed: 34990285) [IF=2.9]

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.