产品: Mre11 抗体
货号: AF5317
描述: Rabbit polyclonal antibody to Mre11
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 80 kDa; 81kD(Calculated).
蛋白号: P49959
RRID: AB_2837802

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(91%)
克隆:
Polyclonal
特异性:
Mre11 Antibody detects endogenous levels of total Mre11.
RRID:
AB_2837802
引用格式: Affinity Biosciences Cat# AF5317, RRID:AB_2837802.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AT like disease; Ataxia telangiectasia disorder like; ATLD; DNA recombination and repair protein; Double strand break repair protein MRE11A; Double-strand break repair protein MRE11A; endo/exonuclease Mre11; HNGS1; meiotic recombination (S. cerevisiae) 11 homolog A; Meiotic recombination 11 homolog 1; meiotic recombination 11 homolog A (S. cerevisiae); Meiotic recombination 11 homolog A; MmMRE11A; Mre 11; MRE 11a; MRE 11b; MRE11 homolog 1; MRE11 homolog A; MRE11 meiotic recombination 11 homolog A (S. cerevisiae); MRE11 meiotic recombination 11 homolog A; MRE11_HUMAN; MRE11A; MRE11b; OTTHUMP00000236830; OTTHUMP00000236831; OTTHUMP00000236832; OTTHUMP00000236833;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
描述:
Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity,
序列:
MSTADALDDENTFKILVATDIHLGFMEKDAVRGNDTFVTLDEILRLAQENEVDFILLGGDLFHENKPSRKTLHTCLELLRKYCMGDRPVQFEILSDQSVNFGFSKFPWVNYQDGNLNISIPVFSIHGNHDDPTGADALCALDILSCAGFVNHFGRSMSVEKIDISPVLLQKGSTKIALYGLGSIPDERLYRMFVNKKVTMLRPKEDENSWFNLFVIHQNRSKHGSTNFIPEQFLDDFIDLVIWGHEHECKIAPTKNEQQLFYISQPGSSVVTSLSPGEAVKKHVGLLRIKGRKMNMHKIPLHTVRQFFMEDIVLANHPDIFNPDNPKVTQAIQSFCLEKIEEMLENAERERLGNSHQPEKPLVRLRVDYSGGFEPFSVLRFSQKFVDRVANPKDIIHFFRHREQKEKTGEEINFGKLITKPSEGTTLRVEDLVKQYFQTAEKNVQLSLLTERGMGEAVQEFVDKEEKDAIEELVKYQLEKTQRFLKERHIDALEDKIDEEVRRFRETRQKNTNEEDDEVREAMTRARALRSQSEESASAFSADDLMSIDLAEQMANDSDDSISAATNKGRGRGRGRRGGRGQNSASRGGSQRGRADTGLETSTRSRNSKTAVSASRNMSIIDAFKSTRQQPSRNVTTKNYSEVIEVDESDVEEDIFPTTSKTDQRWSSTSSSKIMSQSQVSKGVDFESSEDDDDDPFMNTSSLRRNRR

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
91
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P49959 作为底物

Site PTM Type Enzyme
S2 Acetylation
T12 Phosphorylation
K70 Ubiquitination
T74 Phosphorylation
S158 Phosphorylation
K161 Ubiquitination
S165 Phosphorylation
K171 Ubiquitination
S183 Phosphorylation
Y190 Phosphorylation
K196 Acetylation
K197 Acetylation
K197 Ubiquitination
K255 Ubiquitination
S264 Phosphorylation Q13535 (ATR) , Q13315 (ATM)
T272 Phosphorylation
S273 Phosphorylation
S275 Phosphorylation
K282 Ubiquitination
K298 Ubiquitination
T329 Phosphorylation
K339 Ubiquitination
K360 Ubiquitination
S382 Phosphorylation Q13535 (ATR)
K384 Ubiquitination
R388 Methylation
K393 Ubiquitination
K407 Ubiquitination
K416 Acetylation
K416 Ubiquitination
K420 Ubiquitination
T426 Phosphorylation
K434 Ubiquitination
Y436 Phosphorylation
K442 Ubiquitination
S447 Phosphorylation
K464 Ubiquitination
K475 Ubiquitination
K480 Acetylation
K480 Ubiquitination
T481 Phosphorylation
K486 Ubiquitination
K496 Ubiquitination
S531 Phosphorylation Q13535 (ATR)
S558 Phosphorylation
S561 Phosphorylation
R570 Methylation
R572 Methylation
R574 Methylation
R576 Methylation
R577 Methylation
R580 Methylation
R587 Methylation
S590 Phosphorylation
R592 Methylation
R594 Methylation
T597 Phosphorylation
R604 Methylation
S613 Phosphorylation
R616 Methylation
S619 Phosphorylation
K625 Sumoylation
S649 Phosphorylation P68400 (CSNK2A1)
S667 Phosphorylation
S668 Phosphorylation
T669 Phosphorylation
S671 Phosphorylation
S672 Phosphorylation
K673 Ubiquitination
S676 Phosphorylation Q13315 (ATM)
S678 Phosphorylation Q13315 (ATM)
S681 Phosphorylation
S688 Phosphorylation
S689 Phosphorylation
S701 Phosphorylation
S702 Phosphorylation

研究背景

功能:

Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11 to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.

翻译修饰:

Ubiquitinated following DNA damage. Ubiquitination triggers interaction with UBQLN4, leading to MRE11 removal from chromatin and degradation by the proteasome.

细胞定位:

Nucleus. Chromosome>Telomere. Chromosome.
Note: Localizes to discrete nuclear foci after treatment with genotoxic agents.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Component of the MRN complex composed of two heterodimers RAD50/MRE11 associated with a single NBN. As part of the MRN complex, interacts with MCM9; the interaction recruits the complex to DNA repair sites. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11 and NBN. Found in a complex with TERF2. Interacts with DCLRE1C/Artemis and DCLRE1B/Apollo. Interacts with ATF2. Interacts with EXD2. Interacts with MRNIP. Interacts with SAMHD1; leading to stimulate 3'-5' exonuclease activity. Interacts (when ubiquitinated) with UBQLN4 (via its UBA domain).

(Microbial infection) Interacts with herpes simplex virus 1 protein UL12.

蛋白家族:

Belongs to the MRE11/RAD32 family.

研究领域

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Genetic Information Processing > Replication and repair > Homologous recombination.

· Genetic Information Processing > Replication and repair > Non-homologous end-joining.

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