Cleaved-Caspase 6 (Asp179) Antibody - #AF5381

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产品: Cleaved-Caspase 6 (Asp179) 抗体
货号: AF5381
描述: Rabbit polyclonal antibody to Cleaved-Caspase 6 (Asp179)
应用: WB IHC IF/ICC
反应: Human, Rat
预测: Pig, Zebrafish, Bovine, Horse, Dog
分子量: 18 kDa; 33kD(Calculated).
蛋白号: P55212
RRID: AB_2837866

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Rat
预测:
Pig(100%), Zebrafish(83%), Bovine(100%), Horse(100%), Dog(100%)
克隆:
Polyclonal
特异性:
Cleaved-Caspase 6 (Asp179) Antibody detects endogenous levels of fragment of activated Caspase 6 resulting from cleavage adjacent to Asp179.
RRID:
AB_2837866
引用格式: Affinity Biosciences Cat# AF5381, RRID:AB_2837866.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Apoptotic protease Mch-2; Apoptotic protease MCH2; CASP-6; CASP6; CASP6_HUMAN; Caspase 6; Caspase 6 apoptosis related cysteine protease; Caspase 6, apoptosis related cysteine peptidase; Caspase-6 subunit p11; Mch2; OTTHUMP00000162742; OTTHUMP00000162743;

抗原和靶标

免疫原:
Uniprot:

P55212(CASP6_HUMAN) >>Visit HPA database.

基因/基因ID:
CASP6(839)
描述:
Caspase-6 (Mch2) is one of the major executioner caspases functioning in cellular apoptotic processes. Upon apoptotic stimulation, initiator caspases such as caspase-9 are cleaved and activated.
序列:
MSSASGLRRGHPAGGEENMTETDAFYKREMFDPAEKYKMDHRRRGIALIFNHERFFWHLTLPERRGTCADRDNLTRRFSDLGFEVKCFNDLKAEELLLKIHEVSTVSHADADCFVCVFLSHGEGNHIYAYDAKIEIQTLTGLFKGDKCHSLVGKPKIFIIQACRGNQHDVPVIPLDVVDNQTEKLDTNITEVDAASVYTLPAGADFLMCYSVAEGYYSHRETVNGSWYIQDLCEMLGKYGSSLEFTELLTLVNRKVSQRRVDFCKDPSAIGKKQVPCFASMLTKKLHFFPKSN

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Zebrafish
83
Sheep
0
Xenopus
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P55212 作为底物

Site PTM Type Enzyme
R9 Methylation
K27 Ubiquitination
K36 Acetylation
K36 Ubiquitination
S79 Phosphorylation
K92 Ubiquitination
K144 Ubiquitination
K147 Ubiquitination
K154 Ubiquitination
S257 Phosphorylation O60285 (NUAK1)
K265 Ubiquitination
K272 Ubiquitination
K273 Acetylation
K273 Ubiquitination
K284 Acetylation
K285 Acetylation
K285 Ubiquitination

研究背景

功能:

Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death.

翻译修饰:

Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits.

细胞定位:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 11 kDa (p11) subunit. Interacts with BIRC6/bruce.

蛋白家族:

Belongs to the peptidase C14A family.

研究领域

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

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