FABP4 Antibody - #DF6035

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产品: FABP4 抗体
货号: DF6035
描述: Rabbit polyclonal antibody to FABP4
应用: WB IHC
反应: Human, Mouse, Rat
预测: Horse, Rabbit, Dog
分子量: 15kDa; 15kD(Calculated).
蛋白号: P15090
RRID: AB_2838009

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Horse(83%), Rabbit(92%), Dog(92%)
克隆:
Polyclonal
特异性:
FABP4 Antibody detects endogenous levels of total FABP4.
RRID:
AB_2838009
引用格式: Affinity Biosciences Cat# DF6035, RRID:AB_2838009.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

3T3-L1 lipid-binding protein; 422/aP2; A-FABP; adipocyte; Adipocyte lipid binding protein; Adipocyte lipid-binding protein; Adipocyte protein AP2; Adipocyte-type fatty acid-binding protein; AFABP; ALBP; ALBP/Ap2; aP2; Epididymis secretory protein Li 104; FABP; FABP4; FABP4_HUMAN; Fatty acid binding protein 4 adipocyte; Fatty acid binding protein 4; Fatty acid binding protein adipocyte; Fatty acid-binding protein 4; Fatty acid-binding protein; HEL S 104; Lbpl; Myelin P2 protein homolog; P15; P2 adipocyte protein; Protein 422;

抗原和靶标

免疫原:
Uniprot:

P15090(FABP4_HUMAN) >>Visit HPA database.

基因/基因ID:
FABP4(2167)
描述:
Fatty acid binding proteins (FABPs) bind to fatty acids and other lipids to function as cytoplasmic lipid chaperones (1). They participate in the transport of fatty acids and other lipids to various cellular pathways (2). The predominant fatty acid binding protein found in adipocytes is FABP4, also called adipocyte fatty acid binding protein or aP2. FABP4 is also expressed in macrophages (3). FABP4 knockout mice fed a high-fat and high-calorie diet become obese but develop neither insulin resistance nor diabetes, suggesting that this protein might be a link between obesity and insulin resistance and diabetes (4). Mice deficient in both FABP4 and ApoE show protection against atherosclerosis when compared with mice deficient only in ApoE (3). Mice carrying a FABP4 genetic variant exhibit both reduced FABP4 expression and a reduced potential for developing type 2 diabetes and coronary heart disease. A related study in humans indicated a similar pattern, suggesting that FABP4 may be a potential therapeutic target in the treatment of these disorders (1).
序列:
MCDAFVGTWKLVSSENFDDYMKEVGVGFATRKVAGMAKPNMIISVNGDVITIKSESTFKNTEISFILGQEFDEVTADDRKVKSTITLDGGVLVHVQKWDGKSTTIKRKREDDKLVVECVMKGVTSTRVYERA

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Rabbit
92
Dog
92
Horse
83
Bovine
75
Xenopus
58
Pig
0
Sheep
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P15090 作为底物

Site PTM Type Enzyme
C2 Acetylation
T8 Phosphorylation
Y20 Phosphorylation P06213 (INSR)
K101 Acetylation
T103 Phosphorylation
T104 Phosphorylation
Y129 Phosphorylation

研究背景

功能:

Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus.

细胞定位:

Cytoplasm. Nucleus.
Note: Depending on the nature of the ligand, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus. Subject to constitutive nuclear export.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Monomer. Homodimer. Interacts with PPARG (By similarity).

蛋白家族:

Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.

Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.

研究领域

· Organismal Systems > Endocrine system > PPAR signaling pathway.

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

文献引用

1). IL-17A promotes fatty acid uptake through the IL-17A/IL-17RA/p-STAT3/FABP4 axis to fuel ovarian cancer growth in an adipocyte-rich microenvironment. CANCER IMMUNOLOGY IMMUNOTHERAPY (PubMed: 31802182) [IF=5.8]

Application: WB    Species: Mouse    Sample: OvCa cells

Fig. 1 rhIL-17A increased FABP4 expression in OvCa cells via STAT3 signaling. Dose–efect (a, b) and time–efect (c) experiments were performed in A2780 and OVCAR3 cells. a mRNA level of FABP4 after rhIL-17A treatment. b, c Protein expression of FABP4 after rhIL-17A treatment. d-(a) Protein expression of FABP4, p-STAT3 and STAT3 after rhIL-17A and/or STATTIC treatment (A2780: 0.3125  μM; OVCAR3: 1.25  μM). d-(b) The relative expression of proteins in d-(a). Three independent experiments were performed and a representative image is shown. Data represent the mean±SD from three independent experiments. *p<0.05, **p<0.01

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