AKR1A1 Antibody - #DF6213

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产品: AKR1A1 抗体
货号: DF6213
描述: Rabbit polyclonal antibody to AKR1A1
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
分子量: 37kDa; 37kD(Calculated).
蛋白号: P14550
RRID: AB_2838179

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(88%), Xenopus(100%)
克隆:
Polyclonal
特异性:
AKR1A1 Antibody detects endogenous levels of total AKR1A1.
RRID:
AB_2838179
引用格式: Affinity Biosciences Cat# DF6213, RRID:AB_2838179.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AK1A1_HUMAN; Akr1a1; Alcohol dehydrogenase [NADP+]; Alcohol dehydrogenase; Aldehyde reductase; Aldo keto reductase family 1 member A1 (aldehyde reductase); Aldo-keto reductase family 1 member A1; ALDR1; ALR; ARM; DD3; MGC12529; MGC1380;

抗原和靶标

免疫原:
Uniprot:

P14550(AK1A1_HUMAN) >>Visit HPA database.

基因/基因ID:
AKR1A1(10327)
表达:
P14550 AK1A1_HUMAN:

Widely expressed. Highly expressed in kidney, salivary gland and liver. Detected in trachea, stomach, brain, lung, prostate, placenta, mammary gland, small intestine and lung.

描述:
This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member, also known as aldehyde reductase, is involved in the reduction of biogenic and xenobiotic aldehydes and is present in virtually every tissue. Multiple alternatively spliced transcript variants of this gene exist, all encoding the same protein. [provided by RefSeq, Jan 2011]
序列:
MAASCVLLHTGQKMPLIGLGTWKSEPGQVKAAVKYALSVGYRHIDCAAIYGNEPEIGEALKEDVGPGKAVPREELFVTSKLWNTKHHPEDVEPALRKTLADLQLEYLDLYLMHWPYAFERGDNPFPKNADGTICYDSTHYKETWKALEALVAKGLVQALGLSNFNSRQIDDILSVASVRPAVLQVECHPYLAQNELIAHCQARGLEVTAYSPLGSSDRAWRDPDEPVLLEEPVVLALAEKYGRSPAQILLRWQVQRKVICIPKSITPSRILQNIKVFDFTFSPEEMKQLNALNKNWRYIVPMLTVDGKRVPRDAGHPLYPFNDPY

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
88
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P14550 作为底物

Site PTM Type Enzyme
A2 Acetylation
K13 Ubiquitination
K23 Ubiquitination
K30 Ubiquitination
S38 Phosphorylation
Y50 Phosphorylation
K61 Ubiquitination
K68 Ubiquitination
K80 Ubiquitination
K85 Ubiquitination
K127 Acetylation
K127 Ubiquitination
C134 S-Nitrosylation
S137 Phosphorylation
K141 Ubiquitination
K145 Ubiquitination
K153 Ubiquitination
S162 Phosphorylation
T208 Phosphorylation
Y210 Phosphorylation
S211 Phosphorylation
S215 Phosphorylation
S216 Phosphorylation
K263 Ubiquitination
K275 Ubiquitination
T280 Phosphorylation
S282 Phosphorylation
K287 Ubiquitination
K294 Ubiquitination
T304 Phosphorylation
K308 Ubiquitination
Y325 Phosphorylation

研究背景

功能:

Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays enzymatic activity towards endogenous metabolites such as aromatic and aliphatic aldehydes, ketones, monosaccharides and bile acids, with a preference for negatively charged substrates, such as glucuronate and succinic semialdehyde. Functions as a detoxifiying enzyme by reducing a range of toxic aldehydes. Reduces methylglyoxal and 3-deoxyglucosone, which are present at elevated levels under hyperglycemic conditions and are cytotoxic. Involved also in the detoxification of lipid-derived aldehydes like acrolein (By similarity). Plays a role in the activation of procarcinogens, such as polycyclic aromatic hydrocarbon trans-dihydrodiols, and in the metabolism of various xenobiotics and drugs, including the anthracyclines doxorubicin (DOX) and daunorubicin (DAUN). Displays no reductase activity towards retinoids (By similarity).

细胞定位:

Cytoplasm>Cytosol. Apical cell membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Widely expressed. Highly expressed in kidney, salivary gland and liver. Detected in trachea, stomach, brain, lung, prostate, placenta, mammary gland, small intestine and lung.

亚基结构:

Monomer.

蛋白家族:

Belongs to the aldo/keto reductase family.

研究领域

· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.

· Metabolism > Carbohydrate metabolism > Pentose and glucuronate interconversions.

· Metabolism > Lipid metabolism > Glycerolipid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

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