产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6242, RRID:AB_2838208.
展开/折叠
Anterior pharynx defective 2; APH 2; APH2; ATAG1874; KIAA0253; Ncstn; NCT; NICA_HUMAN; Nicastrin; RP11 517F10.1; RP11517F101;
抗原和靶标
Detected in brain (at protein level) (PubMed:10993067). Widely expressed (PubMed:11396676).
- Q92542 NICA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MATAGGGSGADPGSRGLLRLLSFCVLLAGLCRGNSVERKIYIPLNKTAPCVRLLNATHQIGCQSSISGDTGVIHVVEKEEDLQWVLTDGPNPPYMVLLESKHFTRDLMEKLKGRTSRIAGLAVSLTKPSPASGFSPSVQCPNDGFGVYSNSYGPEFAHCREIQWNSLGNGLAYEDFSFPIFLLEDENETKVIKQCYQDHNLSQNGSAPTFPLCAMQLFSHMHAVISTATCMRRSSIQSTFSINPEIVCDPLSDYNVWSMLKPINTTGTLKPDDRVVVAATRLDSRSFFWNVAPGAESAVASFVTQLAAAEALQKAPDVTTLPRNVMFVFFQGETFDYIGSSRMVYDMEKGKFPVQLENVDSFVELGQVALRTSLELWMHTDPVSQKNESVRNQVEDLLATLEKSGAGVPAVILRRPNQSQPLPPSSLQRFLRARNISGVVLADHSGAFHNKYYQSIYDTAENINVSYPEWLSPEEDLNFVTDTAKALADVATVLGRALYELAGGTNFSDTVQADPQTVTRLLYGFLIKANNSWFQSILRQDLRSYLGDGPLQHYIAVSSPTNTTYVVQYALANLTGTVVNLTREQCQDPSKVPSENKDLYEYSWVQGPLHSNETDRLPRCVRSTARLARALSPAFELSQWSSTEYSTWTESRWKDIRARIFLIASKELELITLTVGFGILIFSLIVTYCINAKADVLFIAPREPGAVSY
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q92542 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| N45 | N-Glycosylation | Uniprot | |
| N55 | N-Glycosylation | Uniprot | |
| K101 | Ubiquitination | Uniprot | |
| K110 | Acetylation | Uniprot | |
| K110 | Ubiquitination | Uniprot | |
| K112 | Acetylation | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| N187 | N-Glycosylation | Uniprot | |
| N264 | N-Glycosylation | Uniprot | |
| K349 | Acetylation | Uniprot | |
| K351 | Ubiquitination | Uniprot | |
| N387 | N-Glycosylation | Uniprot | |
| K403 | Ubiquitination | Uniprot | |
| S425 | Phosphorylation | Uniprot | |
| S426 | Phosphorylation | Uniprot | |
| R434 | Methylation | Uniprot | |
| N435 | N-Glycosylation | Uniprot | |
| S437 | Phosphorylation | O00141 (SGK1) | Uniprot |
| N464 | N-Glycosylation | Uniprot | |
| N506 | N-Glycosylation | Uniprot | |
| N530 | N-Glycosylation | Uniprot | |
| N562 | N-Glycosylation | Uniprot | |
| N573 | N-Glycosylation | Uniprot | |
| N580 | N-Glycosylation | Uniprot | |
| N612 | N-Glycosylation | Uniprot | |
| S632 | Phosphorylation | Uniprot | |
| S638 | Phosphorylation | Uniprot | |
| T647 | Phosphorylation | Uniprot | |
| T649 | Phosphorylation | Uniprot | |
| T672 | Phosphorylation | Uniprot | |
| T674 | Phosphorylation | Uniprot | |
| S708 | Phosphorylation | Uniprot |
研究背景
Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein). The gamma-secretase complex plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels.
N-glycosylated.
Membrane>Single-pass type I membrane protein. Cytoplasmic vesicle membrane>Single-pass type I membrane protein. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Detected in brain (at protein level). Widely expressed.
Component of the gamma-secretase complex. The functional gamma-secretase complex is composed of at least four polypeptides: a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PSENEN/PEN2. Binds to proteolytic processed C-terminal fragments C83 and C99 of the amyloid precursor protein (APP). Interacts with PSEN1 and PSEN2.
Belongs to the nicastrin family.
研究领域
· Environmental Information Processing > Signal transduction > Notch signaling pathway. (View pathway)
· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.
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