PLA2G2A Antibody - #DF6366

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产品: PLA2G2A 抗体
货号: DF6366
描述: Rabbit polyclonal antibody to PLA2G2A
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Horse, Rabbit
分子量: 16kDa; 16kD(Calculated).
蛋白号: P14555
RRID: AB_2838330

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 100ul RMB¥ 2300 现货
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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(80%), Horse(90%), Rabbit(80%)
克隆:
Polyclonal
特异性:
PLA2G2A Antibody detects endogenous levels of total PLA2G2A.
RRID:
AB_2838330
引用格式: Affinity Biosciences Cat# DF6366, RRID:AB_2838330.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

EC 3.1.1.4; GIIC sPLA2; Group IIA phospholipase A2; membrane associated; MOM1; Non pancreatic secretory phospholipase A2; Non-pancreatic secretory phospholipase A2; NPS PLA2; NPS-PLA2; NPSPLA2; PA2GA_HUMAN; Phosphatidylcholine 2 acylhydrolase; Phosphatidylcholine 2-acylhydrolase 2A; Phospholipase A2; Phospholipase A2 group IIA (platelets synovial fluid); Phospholipase A2 membrane associated precursor; PLA2; PLA2B; PLA2G2A; PLA2L; PLA2S; PLAS1; RASF A; RASFA; sPLA2;

抗原和靶标

免疫原:
Uniprot:

P14555(PA2GA_HUMAN) >>Visit HPA database.

基因/基因ID:
PLA2G2A(5320)
描述:
The protein encoded by this gene is a member of the phospholipase A2 family (PLA2). PLA2s constitute a diverse family of enzymes with respect to sequence, function, localization, and divalent cation requirements. This gene product belongs to group II, which contains secreted form of PLA2, an extracellular enzyme that has a low molecular mass and requires calcium ions for catalysis. It catalyzes the hydrolysis of the sn-2 fatty acid acyl ester bond of phosphoglycerides, releasing free fatty acids and lysophospholipids, and thought to participate in the regulation of the phospholipid metabolism in biomembranes. Several alternatively spliced transcript variants with different 5' UTRs have been found for this gene.[provided by RefSeq, Sep 2009]
序列:
MKTLLLLAVIMIFGLLQAHGNLVNFHRMIKLTTGKEAALSYGFYGCHCGVGGRGSPKDATDRCCVTHDCCYKRLEKRGCGTKFLSYKFSNSGSRITCAKQDSCRSQLCECDKAAATCFARNKTTYNKKYQYYSNKHCRGSTPRC

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
90
Pig
80
Rabbit
80
Bovine
70
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P14555 作为底物

Site PTM Type Enzyme
K82 Ubiquitination
Y86 Phosphorylation
K128 Sumoylation

研究背景

功能:

Catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Thought to participate in the regulation of phospholipid metabolism in biomembranes including eicosanoid biosynthesis. Independent of its catalytic activity, acts as a ligand for integrins. Binds to and activates integrins ITGAV:ITGB3, ITGA4:ITGB1 and ITGA5:ITGB1. Binds to a site (site 2) which is distinct from the classical ligand-binding site (site 1) and induces integrin conformational changes and enhanced ligand binding to site 1. Induces cell proliferation in an integrin-dependent manner.

细胞定位:

Cell membrane>Peripheral membrane protein. Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
蛋白家族:

Belongs to the phospholipase A2 family.

研究领域

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Metabolism > Lipid metabolism > Glycerophospholipid metabolism.

· Metabolism > Lipid metabolism > Ether lipid metabolism.

· Metabolism > Lipid metabolism > Arachidonic acid metabolism.

· Metabolism > Lipid metabolism > Linoleic acid metabolism.

· Metabolism > Lipid metabolism > alpha-Linolenic acid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

· Organismal Systems > Digestive system > Pancreatic secretion.

· Organismal Systems > Digestive system > Fat digestion and absorption.

文献引用

1). Phospholipase A2 regulates autophagy in gouty arthritis: proteomic and metabolomic studies. Journal of Translational Medicine (PubMed: 37069596) [IF=7.4]

Application: WB    Species: Human    Sample:

Fig. 6 Interactions between the proteome and metabolome, and validation of the autophagy pathway. A: Example diagram of protein and metabolism KGML network. Squares represent pathways, triangles represent proteins, and circles represent metabolites. Red indicates up-regulated proteins or green indicates down-regulated proteins or metabolites. B: The expressions of LC3B and SQSTM1/62 were evaluated by western blotting. C: The expressions of PLA2 were evaluated by western blotting. D-F: Quantification of LC3B, SQSTM1/62 and PLA2 expressions (n = 3, all the data are expressed as means ± SD, two-way ANOVA followed by Turkey's post hoc test was applied)
2). Identification and immunological characterization of PLA2G2A and cell death-associated molecular clusters in idiopathic pulmonary fibrosis. Life Sciences (PubMed: 37673297) [IF=6.1]

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