MIF Antibody - #DF6404

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产品: MIF 抗体
货号: DF6404
描述: Rabbit polyclonal antibody to MIF
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
分子量: 12kDa; 12kD(Calculated).
蛋白号: P14174
RRID: AB_2838367

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MS Report
HPLC Report
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(90%), Zebrafish(89%), Bovine(90%), Horse(90%), Sheep(90%), Rabbit(90%), Chicken(90%), Xenopus(90%)
克隆:
Polyclonal
特异性:
MIF Antibody detects endogenous levels of total MIF.
RRID:
AB_2838367
引用格式: Affinity Biosciences Cat# DF6404, RRID:AB_2838367.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

GIF; GLIF; Glycosylation inhibiting factor; Glycosylation-inhibiting factor; L-dopachrome isomerase; L-dopachrome tautomerase; Macrophage migration inhibitory factor (glycosylation-inhibiting factor); Macrophage migration inhibitory factor; MIF; MIF protein; MIF_HUMAN; MMIF; Phenylpyruvate tautomerase;

抗原和靶标

免疫原:
Uniprot:

P14174(MIF_HUMAN) >>Visit HPA database.

基因/基因ID:
MIF(4282)
描述:
Macrophage migration inhibitory factor, known as MIF or glycosylation-inhibiting factor, is a secreted, homotrimeric, pro-inflammatory cytokine that modulates macrophage and T cell function and is an important regulator of host response to infection. MIF is expressed at sites of inflammation, which suggests that it plays a role in regulating macrophage function in host defense. MIF is produced by the pituitary gland and is found in monocytes, macrophages, differentiating immunological cells in the eye lens and brain, and fibroblasts. Elevated levels of MIF protein are detected in the plasma of patients with severe sepsis or septic shock, a condition where MIF influences endotoxic shock by enhancing the production of other inflammatory cytokines including tumor necrosis factor (TNF ), interleukin-1 (IL-1) and interferon- (IFN- ). MIF promotes the systemic inflammatory response by counter-regulating glucocorticoid-mediated inhibition of immune-cell activation and proinflammatory cytokine production. MIF may mediate tissue destruction through the induction of proteinases.
序列:
MPMFIVNTNVPRASVPDGFLSELTQQLAQATGKPPQYIAVHVVPDQLMAFGGSSEPCALCSLHSIGKIGGAQNRSYSKLLCGLLAERLRISPDRVYINYYDMNAANVGWNNSTFA

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
90
Horse
90
Bovine
90
Sheep
90
Xenopus
90
Chicken
90
Rabbit
90
Zebrafish
89
Dog
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P14174 作为底物

Site PTM Type Enzyme
T8 Phosphorylation
R12 Methylation
S14 Phosphorylation
S21 Phosphorylation
Y37 Phosphorylation
C60 S-Nitrosylation
R74 Methylation
S75 Phosphorylation
K78 Acetylation
K78 Ubiquitination
C81 S-Nitrosylation
R87 Methylation
R89 Methylation
S91 Phosphorylation
R94 Methylation
Y99 Phosphorylation
Y100 Phosphorylation
S112 O-Glycosylation
T113 O-Glycosylation

研究背景

功能:

Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.

细胞定位:

Secreted. Cytoplasm.
Note: Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Homotrimer. Interacts with CXCR2 extracellular domain (By similarity). Interacts with the CD74 extracellular domain, COPS5 and BNIPL.

蛋白家族:

Belongs to the MIF family.

研究领域

· Metabolism > Amino acid metabolism > Tyrosine metabolism.

· Metabolism > Amino acid metabolism > Phenylalanine metabolism.

文献引用

1). Allyl isothiocyanate mitigates airway inflammation and constriction in a house dust mite-induced allergic asthma model via upregulation of tight junction proteins and the TRPA1 modulation. Biomedicine & Pharmacotherapy (PubMed: 37634475) [IF=7.5]

Application: WB    Species: Mouse    Sample:

Fig. 3. Effect of AITC on airway inflammation in HDM-induced asthma mice. Treat with AITC significantly decreased the level of (A) HDM-specific IgE, (B) IL-4, (C) IL-5, (D) IL-13 and (E) MIF. (F) Histology image of H&E stained lung sections and (G) mean inflammatory index for each sample in the same vision area is a measure of the severity of inflammation ranging from 0 to 3. The upper and lower vision areas are magnified at 200 × and 400 ×, respectively. Data are expressed in mean ± SEM (n = 5–10). Significant difference presented as different symbol. *p 
2). Screening of immunosuppressive factors for biomarkers of breast cancer malignancy phenotypes and subtype-specific targeted therapy. PeerJ (PubMed: 31293831) [IF=2.7]

Application: IHC    Species: human    Sample: breast

Figure 7 Immunohistochemical detection of the expression of MIF and VEGFA in a breast cancer tissue microarray. (A, D) Negative expression (-) in cancer-adjacent normal breast tissue.
3). Screening of Immunosuppressive Factors for Identifying Breast Cancer Malignant Phenotypes Using mRNA Microarray Datasets.

Application: IHC    Species: Human    Sample: breast cancer

Figure 3 Immunohistochemical detection of MIF (Row 1) and VEGFA (Row 2) in breast cancer tissue microarray.(a) and (D), Negative expression in Cancer adjacent normal breast tissue. (b) and (e), Negative expression in Invasive ductal carcinoma. (c) and (f), Positive expression in Invasive ductal carcinoma.

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